Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

Levay, Konstantin; Завриев, С. К.

doi:10.1099/0022-1317-72-10-2333

Cited by 34 publications

(12 citation statements)

References 20 publications

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“…CVB belongs to the genus Carlavirus, which comprises 35 definitive species and 29 tentative members with conserved genome organization (Levay and Zavriev, 1991;Adams et al, 2004;Martelli et al, 2007). Their positive-stranded genomic RNA encodes six genes, of which the gene for p12 occupies the extreme 39-terminal position (see Supplemental Figure 1A online; Adams et al, 2004;Martelli et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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Plant viruses cause a variety of diseases in susceptible hosts. The disease symptoms often include leaf malformations and other developmental abnormalities, suggesting that viruses can affect plant development. However, little is known about the mechanisms underlying virus interference with plant morphogenesis. Here, we show that a C-4 type zinc-finger (ZF) protein, p12, encoded by a carlavirus (chrysanthemum virus B) can induce cell proliferation, which results in hyperplasia and severe leaf malformation. We demonstrate that the p12 protein activates expression of a regulator of cell size and proliferation, designated upp-L (upregulated by p12), which encodes a transcription factor of the basic/helix-loop-helix family sufficient to cause hyperplasia. The induction of upp-L requires translocation of the p12 protein into the nucleus and ZF-dependent specific interaction with the conserved regulatory region in the upp-L promoter. Our results establish the role of the p12 protein in modulation of host cell morphogenesis. It is likely that other members of the conserved C-4 type ZF family of viral proteins instigate reprogramming of plant development by mimicking eukaryotic transcriptional activators.

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Section: Introductionmentioning

confidence: 99%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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“…The double-stranded eDNA for the ShVX RNA was synthesized essentially as described previously (Levay & Zavriev, 1991). The cDNA was ligated to a Sinai-digested pGEM-7Zf(+) (Promega) or HinclI-digested pGEM-3Z plasmid and transformed into competent Escherichia coli XL-1B cells (Stratagene Gene Cloning System).…”

Section: Methodsmentioning

confidence: 99%

“…Virus-specific RNAs were revealed with a heavily 32p-labelled RNA transcript complementary to the T-proximal region of ShVX RNA which was synthesized using phage SP6 RNA polymerase on the pX23 plasmid (cDNA insert 8336 to 8890; Fig. 1 Levay & Zavriev, 1991), potato aucuba mosaic virus (PAMV; Bundin et al, 1986), potato virus X (PVX; Huisman et al, 1988), white clover mosaic virus (WCIMV;Forster et al, 1988), narcissus mosaic virus (N MV;Zuidema et al, 1989), papaya mosaic virus (PMV; Sit et al, 1989), lily virus X (LVX; Memelink et al, 1990), clover yellow mosaic virus (CYMV; Sit et al, 1990), strawberry mild yellow edge-associated potexvirus (SMYEV;Jelkmann et al, 1990), turnip yellow mosaic virus (TYMV; Morch et al, 1988) and apple chlorotic leaf spot virus (ACLSV ;German et al, 1990) were predicted from the nucleotide sequences of the viral RNAs. The initial sequence alignments of the virus proteins were made with the MULTALIN program (Corpet, 1988).…”

Section: Methodsmentioning

confidence: 99%

Nucleotide Sequence of Shallot Virus X RNA Reveals a 5'-proximal Cistron Closely Related to Those of Potexviruses and a Unique Arrangement of the 3'-proximal Cistrons

Kanyuka

Vishnichenko

Levay

et al. 1992

Journal of General Virology

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The 8890 nucleotide RNA sequence of shallot virus X (ShVX), a new virus isolated from shallot, has been determined. The sequence contains six open reading frames (ORFs) which encode putative proteins (in the 5' to 3' direction) of Mr 194528 (ORF1), 26333 (ORF2), 11245 (ORF3), 42209 (ORF4), 28486 (ORF5) and 14741 (ORF6). The ORF1 protein was found to be highly homologous to the putative potexvirus RNA replicases; ORF2, -3, -5 and -6 proteins also have analogues among the potex-and/or carlavirus-encoded proteins. ORF3 is followed by an AUG-lacking frame coding for an amino acid sequence homologous to that of the 7K to 8K proteins of the triple gene block of the above-mentioned viruses. The putative ORF4 protein has no reliable homology with proteins in the database. The results obtained testify that, except for the unique 42K protein gene, the ShVX genome combines a number of elements typical of both carla-and potexviruses.

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“…Partial nucleotide sequences of several carlaviruses have been published (MacKenzie et al, 1989;Foster et al, 1990;Haylor et al, 1990;Memelink et al, 1990;Monis & de Zoeten, 1990;Foster & Mills, 1991Levay & Zavriev, 1991 ;Meehan & Mills, 1991 ;Henderson et al, 1992); however only the BBScV (Cavileer et al, 1994) and potato virus M (PVM) (Zavriev et al, 1991) genomes have been sequenced in their entirety. The BBScV genome is 8514 nucleotides in length excluding the poly(A) tail at the 3' terminus.…”

mentioning

confidence: 99%

“…The complete nucleotide sequence of BBScV has revealed that it contains six open reading frames (ORFs), with the coding potential for proteins homologous to the six deduced PVM products (Zavriev et al, 1991 ;Cavileer et al, 1994). ORF 1 encodes a deduced protein of 223K that contains putative methyl transferase, helicase and polymerase domains typical of those found in proteins involved in RNA replication (Koonin & Dolja, 1993).…”

mentioning

confidence: 99%

Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro

Lawrence

Hillman

1994

Journal of General Virology

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Blueberry scorch carlavirus (BBScV) is a filamentous virus with a polyadenylated, positive-sense RNA genome. A near full-length cDNA clone of BBScV was constructed by assembly of clones from a cDNA library. To generate a full-length cDNA clone, the 5' terminus was mutagenized by PCR to introduce nucleotides present in the wild-type virus and not in the near fulllength clone, and then fused directly to the T7 bacteriophage RNA polymerase promoter at the 5' terminus. Capped and uncapped BBScV transcripts were synthesized in vitro from the full-length cDNA clone. Capped transcripts were infectious, producing systemic symptoms identical to those caused by the wild-type virus following inoculation onto Chenopodium quinoa leaves. Uncapped transcripts were substantially less infectious than capped transcripts. This represents the first report of infectious transcripts for a member of the carlavirus group.Blueberry scorch virus (BBScV) is a member of the carlavirus group of positive-sense ssRNA viruses. Partial nucleotide sequences of several carlaviruses have been published (MacKenzie et al

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Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

Cited by 34 publications

References 20 publications

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

Nucleotide Sequence of Shallot Virus X RNA Reveals a 5'-proximal Cistron Closely Related to Those of Potexviruses and a Unique Arrangement of the 3'-proximal Cistrons

Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro

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