Nucleotide Sequence of Shallot Virus X RNA Reveals a 5'-proximal Cistron Closely Related to Those of Potexviruses and a Unique Arrangement of the 3'-proximal Cistrons

Abstract

The 8890 nucleotide RNA sequence of shallot virus X (ShVX), a new virus isolated from shallot, has been determined. The sequence contains six open reading frames (ORFs) which encode putative proteins (in the 5' to 3' direction) of Mr 194528 (ORF1), 26333 (ORF2), 11245 (ORF3), 42209 (ORF4), 28486 (ORF5) and 14741 (ORF6). The ORF1 protein was found to be highly homologous to the putative potexvirus RNA replicases; ORF2, -3, -5 and -6 proteins also have analogues among the potex-and/or carlavirus-encoded proteins. ORF3 is followed by an AUG-lacking frame coding for an amino acid sequence homologous to that of the 7K to 8K proteins of the triple gene block of the above-mentioned viruses. The putative ORF4 protein has no reliable homology with proteins in the database. The results obtained testify that, except for the unique 42K protein gene, the ShVX genome combines a number of elements typical of both carla-and potexviruses.

Nucleotide sequences of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex- and carlaviruses

“…Additionally the GAAATGG is in good context for functional initiation sites with a G in position -3 and a purine at +4 (Kozak, 1987). As presented by Kanyuka et al (1992) in alignments of ShVX, potex-, carla-and tymoviruses and ACLSV, the motifs of the NTPdependent helicase (Habili & Symons, 1989) and the RNA polymerase (Argos, 1988) can be observed (alignments not shown).…”

“…Five separate potexviruses revealed non-translated regions of 80 to 107 nucleotides (summarized in Jelkmann et al, 1992b). Shallot virus X (ShVX), a virus combining elements of carla-and potexviruses, has 98 5' non-coding nucleotides (Kanyuka et al, 1992). Therefore the number of nucleotides not determined at the 5' terminus of ASPV is probably less than 1% of the total sequence.…”

Diagnóstico biológico e molecular e análise da seqüência de nucleotídeos do gene da proteína capsidial de um isolado do Apple stem pitting virus

“…deduzido da seqüência de aminoácidos da subunidade protéica do ASPV e os valores observados em western blot, os autores consideram-nos compatíveis. Similarmente ao que se constata no presente estudo, a diferença entre o valor calculado e o observado foi atribuída à alta hidrofilicidade da proteína capsidial de ASPV, a exemplo de discrepância similar já observada com a proteína capsidial de Shallot virus X (ShVX), família Flexiviridae gênero Allexivirus, sugerida por Kanyuka et al (1992), que determina um aumento aparente do tamanho da molécula em conseqüência de uma redução da mobilidade eletroforética. As mutações do ASPV mencionadas acima não afetaram, aparentemente, TABELA 2 -Comparação de identidade (%) das seqüências de nucleotídeos (diagonal superior) e de aminoácidos deduzidos (diagonal inferior) do gene da proteína capsidial do Apple stem pitting virus BR1 com as respectivas seqüências de isolados disponíveis em bancos de dados Números de acesso no GenBank : (1) AY572458/AAS78637; (2) D21829/BAA04853; (3) D21828/BAA04852, (4) AF345893/ AAK30563; (5) AF345894/AAK30565; (6) AF345895/AAK30566; (1), (3), (4), (5) determinantes antigênicos, uma vez que o ASPV BR1 reagiu positivamente com ambos os anti-soros produzidos contra isolados europeus em dot-ELISA e imunoblot.…”

Sequence Comparison and Phylogeny of Nucleotide Sequence of Coat Protein and Nucleic Acid Binding Protein of a Distinct Isolate of Shallot virus X from India

“…It is a characteristic feature of viruses in Allexivirus, Carlavirus, Mandarivirus, and Vitivirus. The last Cys residue is, however, replaced by a His residue as has been observed in other ShVX isolates [4].…”