Nucleotide sequence and genornic organization of aedes densonucleosis virus

Afanasiev, Boris N.; Galyov, Edouard E.; Buchatsky, L.; Kozlov, Yuri

doi:10.1016/0042-6822(91)90780-f

Cited by 90 publications

(66 citation statements)

References 53 publications

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“…The putative product of ORF3 revealed similarities with the non-structural protein NS-1 of PfDNV, DsDNV, JcDNV and PcDNV (37, 38, 38 and 33 % identity in a cluster of 200 aa, respectively). Indeed, the C-terminal region of the putative ORF3 product (aa 334-432) contains motifs characteristic of the NTP-binding and helicase domains of the NS-1 polypeptide shared by all parvoviruses (Afanasiev et al, 1991; (Fig. 3b).…”

Section: Orfs 1-3mentioning

confidence: 99%

“…The overall genome organization of DNVs reveals striking differences both among themselves and with the genome organization of vertebrate parvoviruses (Bando et al, 1987;Dumas et al, 1992;Afanasiev et al, 1991). Based on the characteristics of the genome structure, DNVs are subdivided into three genera: Densovirus, Iteravirus and Brevidensovirus.…”

Section: Introductionmentioning

confidence: 99%

“…Viruses that belong to this family have generally two to four capsid proteins: VP1 (80-96 kDa), VP2 (64-85 kDa), VP3 (60-75 kDa) and VP4 (49-52 kDa) (van Regenmortel et al, 2000). Common symptoms resulting from a densovirus infection include hypertrophy of infected nuclei, progressive paralysis and death of the insect host (Chao et al, 1985;Kawase, 1985).The overall genome organization of DNVs reveals striking differences both among themselves and with the genome organization of vertebrate parvoviruses (Bando et al, 1987;Dumas et al, 1992;Afanasiev et al, 1991). Based on the characteristics of the genome structure, DNVs are subdivided into three genera: Densovirus, Iteravirus and Brevidensovirus.…”

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A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus FN1

Munster¹,

Dullemans²,

Verbeek³

et al. 2003

Journal of General Virology

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A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus M. van The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 59 half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus Densovirus. ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus Densovirus. These data indicate that this virus is a new species of the genus Densovirus in the family Parvoviridae. The virus was tentatively named Myzus persicae densovirus. INTRODUCTIONSeveral virus agents that infect arthropods have been identified. So far, all the viruses known to infect aphid species are small isometric RNA viruses (Parrish & Briggs, 1966;D'Arcy et al., 1981; Williamson et al., 1988). Recently, we have isolated from the aphid Myzus persicae a DNA virus that shares the properties of the subfamily Densovirinae. The icosahedral virus particles were 20 nm in diameter and contained a single-stranded DNA genome of approximately 5?7 kb (unpublished results).Among arthropod hosts, representatives of at least five orders from the class Insecta (Lepidoptera, Diptera, Orthoptera, Odonata and Hemiptera) and one order from the class Crustacea (Shike et al., 2000) are known to be infected by densoviruses (DNVs) (Tijssen & Bergoin, 1995;Thao et al., 2001). DNVs (family Parvoviridae) are small non-enveloped viruses of 18-26 nm in diameter containing a single-stranded DNA genome of 4-6 kb (van Regenmortel et al., 2000). Viruses that belong to this family have generally two to four capsid proteins: VP1 (80-96 kDa), VP2 (64-85 kDa), VP3 (60-75 kDa) and VP4 (49-52 kDa) (van Regenmortel et al., 2000). Common symptoms resulting from a densovirus infection include hypertrophy of infected nuclei, progressive paralysis and death of the insect host (Chao et al., 1985;Kawase, 1985).The overall genome organization of DNVs reveals striking differences both among themselves and with the genome organization of vertebrate parvoviruses (Bando et al., 1987;Dumas et al., 1992;Afanasiev et al., 1991). Based on the characteristics of the genome structure, DNVs are subdivided into three genera: Densovirus, Iteravirus and Brevidensovirus. In vertebrate parvoviruses and in DNVs of the genera Iteravirus and Brevidensovirus, the coding sequences of all viral proteins are located on one strand of the viral genome, which is by convention the viral (minus) strand (Berns, 1990). Two sets of open reading frames (ORFs) can be ...

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Section: Orfs 1-3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus FN1

Munster¹,

Dullemans²,

Verbeek³

et al. 2003

Journal of General Virology

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“…This ORF encodes a protein of 666 amino acids, with a molecular weight of 75.77 kDa [69]. The ORF1 protein sequence contains highly conserved replication initiator motifs (rolling-circle replication (RCR) motifs) and NTPbinding and helicase domains (ATPase motifs) common to all parvoviruses which is located between 257 and 315 aa and 480 and 579 aa, respectively [1,10,69,74]. The mid ORF of Indian IHHNV strain consists of 1,092 bp.…”

Section: Genome Organizationmentioning

confidence: 99%

Genomics, Molecular Epidemiology and Diagnostics of Infectious hypodermal and hematopoietic necrosis virus

et al. 2012

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Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is one of the major viral pathogens of penaeid shrimps worldwide, which has resulted in severe mortalities of up to 90 % in cultured Penaeus (Litopenaeus) stylirostris from Hawaii and hence designated Penaeus stylirostris densovirus (PstDNV). IHHNV is distributed in shrimp culture facilities worldwide. It causes large economic loss to the shrimp farming industry. Our knowledge about the natural reservoirs of IHHNV is still scarce. Recent studies suggest that there is sufficient sequence variation among the isolates from different locations in Asia, suggesting multiple geographical strains of the virus. Four complete genomes and several partial sequences of the virus are available in the GenBank. Complete genome information would be useful for assessing the specificity of diagnostics for viruses from different geographical areas. Comparisons of complete genome sequences will help us gain insights into point mutations that can affect virulence of the virus. In addition, because of unavailability of shrimp cell lines for culturing IHHNV in vitro, quantification of virus is difficult. The recent progress in research regarding clinical signs, geographical distribution, complete genome sequence and genetic variation, transmission has made it possible to obtain information on IHHNV. A comprehensive understanding of IHHNV infection process, pathogenesis, structural proteins and replication is essential for developing prevention measures. To date, no effective prophylactic measure for IHHNV infection is available for shrimp to reduce its impact. This review provides an overview of key issues regarding IHHNV infection and disease in commercially important shrimp species.

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“…edu/applications/mfold/cgi-bin/dna-form1.cgi) was used to predict the secondary structure of the BR/07 MDV 5' and 3' ends. For comparison, the same procedure was used to predict the secondary structure of Aedes densonucleosis virus (GenBank accession M37899), which has previously been described (Afanasiev et al 1991). The default settings were used for all of the analyses.…”

Section: Cells and Viruses -The Aedes Albopictus Cell Linementioning

confidence: 99%

Genetic and biological characterization of a densovirus isolate that affects dengue virus infection

Mosimann

Bordignon

Mazzarotto

et al. 2011

Mem. Inst. Oswaldo Cruz

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Brevidensoviruses have an encapsidated, single-stranded DNA genome that predominantly has a negative polarity. In recent years, they have received particular attention due to their potential role in the biological control of pathogenic arboviruses and to their unnoticed presence in cell cultures as contaminants. In addition, brevidensoviruses may also be useful as viral vectors. This study describes the first genetic and biological characterization of a mosquito densovirus that was isolated in Brazil; moreover, we examined the phylogenetic relationship between this isolate and the other brevidensoviruses. We further demonstrate that this densovirus has the potential to be used to biologically control dengue virus (DENV) infection with in vitro co-infection experiments. The present study provides evidence that this densovirus isolate is a fast-spreading virus that affects cell growth and DENV infection

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Nucleotide sequence and genornic organization of aedes densonucleosis virus

Cited by 90 publications

References 53 publications

A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus FN1

A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus FN1

Genomics, Molecular Epidemiology and Diagnostics of Infectious hypodermal and hematopoietic necrosis virus

Genetic and biological characterization of a densovirus isolate that affects dengue virus infection

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