Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71

Chatterjee, Arun; McEvoy, J.; Chambost, Jean-Pierre; Blasco, Francis

doi:10.1128/jb.173.5.1765-1769.1991

Cited by 42 publications

(24 citation statements)

References 31 publications

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“…carotovora 71 was repressed by the E. coli IxA+4, Pnl production was not inhibited in the presence of the lexA4 DNA (14). Furthermore, pnlU contained no sequence resembling a LexA binding site (7) and was expressed only at a basal level in a recA+ lexcA+ E. coli strain in the presetice of DNA-damaging agents (13). Collectively, these findings suggested the requirement of an activator in the regulation of pnlA.…”

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confidence: 73%

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Genetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents

1992

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In Erwinia carotovora subsp. carotovora 71, the induction of pectin lyase (Pnl), the bacteriocin carotovoricin (Ctv), and cellular lysis (Lss) requires a RecA function. We obtained mutants wherein a pleiotropic defect, i.e., the lack of induction with mitomycin C, is not restored by the recA+ DNA. From a genomic library of strain 71, a cosmid (pAKC280) that restored induction of Pnl, Ctv, and Lss by mitomycin C was isolated. The activator function, designated Rdg for regulator of damage-inducible genes, was localized by subcloning and insertional mutagenesis to a 2.6-kb region within a 6.7-kb EcoRI fragment. An rdg-lacZ operon fusion was inducible by mitomycin C in RecA+ but not RecA-derivatives of E. carotovora subsp. carotovora 71 and Escherichia coli. A RecA' E. coli strain carrying only a PnlA+ plasmid was not inducible for Pnl production; however, when both a PnlA+ plasmid and a Rdg+ plasmid were present, the transcription of pnL4 and the production of the enzyme were activated by mitomycin C. The size of the pnL4 transcript produced in E. coli was identical to that of the transcript produced by E. carotovora subsp. carotovora 71, suggesting that the same promoter and termination sequences were being utilized in these bacteria.

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confidence: 73%

“…carotovora 71 were hybridized with a 0.39-kb PvuII-KpnI fragment internal to pnl (7). The amount of the pnlA transcript increased in was carried out as previously described (13).…”

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confidence: 99%

Genetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents

1992

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“…We found sequence similarity between the predicted product of or12 and aspartate racemase of S. thermophilus, as well as full and partial uncharacterized open reading frames from E. coli (44) and E. carotovora (12), respectively (Fig. 5).…”

Section: Discussionmentioning

confidence: 98%

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis pbpE operon, which codes for penicillin-binding protein 4* and an apparent amino acid racemase

Popham

Setlow

1993

J Bacteriol

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Penicillin-binding protein 4* (PBP 4*) was purified from Bacillus subtilis, its N-terminal sequence was determined, and the coding gene, termed pbpE, was cloned and sequenced. The predicted amino acid sequence of PBP 4* exhibited similarity to those of other penicillin-recognizing enzymes. Downstream ofpbpE there was a second gene, termed orfJ, which exhibited sequence similarity with aspartate racemase. The two genes were found to constitute an operon adjacent to and divergently transcribed from the sacB gene at 2960 on the chromosomal map. A weak 1-lactamase activity was associated with PBP 4*, but no enzymatic activity was found for the product oforJ2. Mutation ofpbpE, orf2, or both genes resulted in no observable effect on growth, sporulation, spore heat resistance, or spore germination. A translational pbpE-kacZ fusion was weakly expressed during vegetative growth and was significantly induced at the onset of sporulation. This induction depended on the activity of the spoOA product in relieving repression by the abrB repressor. A single transcription start site which was apparently dependent on E&rL was detected upstream ofpbpE.

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“…Nevertheless, the pattern of expression in both cases indicates that a sequence(s) at some distance from the mapped transcription start site(s) and repressor-binding site is required for transcription, suggesting the likelihood of an upstream activating sequence. The combination of both positive and negative LexA-like regulatory factors in the control of expression of DNAdamage inducible genes has been described for the pectin lyase gene of Erwinia carotovora (Chatterjee et al, 1991;McEvoy et al, 1992;Liu et al, 1994) and pyocins of P. aeruginosa (Matsui et al, 1993). An alternative explanation is suggested by the fact that orf2 does not appear to be expressed by RT-PCR analysis, but the CAT fusion within this region had promoter activity.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterial recA is cotranscribed with a potential regulatory gene called recX

Kg¹,

Movahedzadeh²,

et al. 1997

Molecular Microbiology

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SummaryThe recA gene of Mycobacterium smegmatis has been cloned and sequenced. The amino acid sequence of the RecA protein is highly homologous to other RecA proteins. Three other potential open reading frames were identified. One of these showed extensive homology to a protein, HypB, involved in the incorporation of nickel into hydrogenases. Another, found downstream of and overlapping recA, was similar to a gene, recX, which has been proposed to play a regulatory role related to recA function. The homology between the M. smegmatis sequence and that of Mycobacterium tuberculosis extended upstream of the recA coding region for 140 bp including a motif identical to the Cheo-box consensus sequence which has been shown to bind LexA. In addition, the transcriptional start sites were found to be identical to those identified previously for M. tuberculosis. Transcriptional fusions to the reporter gene chloramphenicol acetyltransferase (CAT) revealed that recA was DNA-damage inducible and that expression required sequences at some distance from the mapped transcriptional start sites. Although a motif with only one mismatch to the Cheo box was found in the intergenic region between orf1 and orf2 these open reading frames were not DNA-damage inducible, nor was this motif required for regulation of recA expression. Gel retardation assays revealed that the reason for this was that LexA did not bind to this sequence containing a mismatch. Reverse transcription/polymerase chain reaction analysis of M. smegmatis RNA demonstrated that recA and orf3 (recX ) are within the same trancriptional unit.

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Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71

Abstract: . 172: [3284][3285][3286][3287][3288][3289] 1990

Cited by 42 publications

References 31 publications

Genetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents

Genetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis pbpE operon, which codes for penicillin-binding protein 4* and an apparent amino acid racemase

Mycobacterial recA is cotranscribed with a potential regulatory gene called recX

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