Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome

Tilakaratne, Nanda; Hardin, Steven E.; Weaver, Robert F.

doi:10.1099/0022-1317-72-2-285

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…This is supported by Northern (RNA) blot analysis (Y. Li, data not shown), which revealed a major late transcript of approximately 1.6 kb, a size consistent with polyadenylation of the 1.3-kb transcript defined by our Si and primer extension data. A 1.6-kb RNA in this region was also reported by Tilakaratne et al (50). The presence of a 2.1-kb RNA in this region was also reported (16); the proposed location of this transcript would predict a tricistronic message including the ORFs upstream and downstream of ctl.…”

Section: Resultssupporting

confidence: 79%

Characterization of a baculovirus gene encoding a small conotoxinlike polypeptide

Eldridge

Miller

1992

J Virol

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We identified a gene of Autographa californica nuclear polyhedrosis virus (AcMNPV) that encodes a small cysteine-rich polypeptide which has size and sequence similarity to omega-conotoxins, a class of calcium ion (Ca2") channel inhibitors, found in the venom of cone snails. Transcriptional analysis indicated that the 159-bp open reading frame, which we named cdl, and a downstream 984-bp open reading frame are transcribed as a single 1.3-kb bicistronic late RNA. The mature ctl gene product was identified as a small secreted protein by high-pressure liquid chromatography fractionation of extraceliular fluid. Viruses with a site-specific deletion in ctl appeared normal with regard to the kinetics and virulence of infection, both in vitro and in vivo. Although we studied the behavior of wild-type and mutant virus-infected insects in some detail, a biological role for ctl in AcMNPV infection remains to be established. * Corresponding author. that the gene product is a small, secreted peptide. The gene is not required for viral infection or replication in vitro or in vivo. We did not observe any difference in the behavior or development of wild-type (wt) or mutant virus-infected insects, and the biological function of ctl remains unknown. MATERIALS AND METHODS Cells and virus. AcMNPV Li (24) was propagated in Spodoptera frugiperda IPLB-SF21 cells (51) as previously described (29). The cells were maintained at 27°C in TC100 medium (GIBCO/BRL, Grand Island, N.Y.) supplemented with 10% fetal bovine serum and 0.26% tryptose broth. RNA isolation. Total RNA was isolated by the guanidinium isothiocyanate method (14) from wt-or AcAINPV-infected SF21 cells. Virus (20 PFU per cell) was allowed to adsorb to cells (107/100-mm plate) for 1 h at room temperature before

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Section: Resultssupporting

confidence: 79%

Characterization of a baculovirus gene encoding a small conotoxinlike polypeptide

Eldridge

Miller

1992

J Virol

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“…HaSNPV lacks a gene for protein tyrosine/serine phosphatase ( ptp ) with dual-specificity (dsPTP) (Tilakaratne et al , 1991 ; Kim & Weaver, 1993 ). This protein specifically removes phosphates from both tyrosine and serine/threonine residues (Wishart et al , 1995 ).…”

Section: Resultsmentioning

confidence: 99%

The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome

Chen

IJkel²,

Tarchini

et al. 2001

Journal of General Virology

200

119

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“…Ac1 encodes a protein tyrosine/serine phosphatase with dual specificity (dsPTP) (Tilakaratne et al , 1991 ; Kim & Weaver, 1993 ). This protein removes phosphates specifically from both tyrosine and serine/threonine residues and regulates the phosphorylation status of a variety of proteins, including growth factors, which in turn regulate developmental processes in living cells (Wishart et al , 1995 ).…”

Section: Resultsmentioning

confidence: 99%

Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome

IJkel

Strien

Heldens

et al. 1999

Journal of General Virology

212

122

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Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome

Cited by 11 publications

References 23 publications

Characterization of a baculovirus gene encoding a small conotoxinlike polypeptide

Characterization of a baculovirus gene encoding a small conotoxinlike polypeptide

The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome

Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome

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