Nucleotide sequence of 5 S RNA from <i>Torulopsis utilis</i>

Nishikawa, Kazuya; Takemura, Shosuke

doi:10.1016/0014-5793(74)80904-x

Cited by 138 publications

(69 citation statements)

References 23 publications

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“…Finally, I would like to mention that my sequence data in the 5-S rRNA coding region confirmed the sequence of 5-S rRNA previously determined by Nishikawa et al [7], except for the order of pyrimidines at positions 33 and 34 (Fig.7).…”

Section: Discussionsupporting

confidence: 87%

“…cerevisiae [5,6]. The sequence of T. utilis 5-S rRNA [7] has been shown to be identical with that of S.cerevisiae 5-S rRNA except for two residues at positions 18 and 61 [8]. I first cloned a segment spanning one repeating unit of the genes for all the four rRNA species (5-S, 5.84, 18-S and 25-S) on an Esclzericlzia coli vector, pBR322, and its structural organization was determined by restriction analysis followed by Southern's hybridization [9].…”

Section: Sequences Were D(t-g-t-a-a-c-c-t) In T Utilis and D(t-a-t-cmentioning

confidence: 99%

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Structure of the 5‐S Ribosomal RNA Gene and Its Adjacent Regions in Torulopsis utilis

Tabata

1980

European Journal of Biochemistry

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A DNA segment spanning one repeating unit of ribosomal RNA(rRNA) genes in a yeast strain, Tovulopsis utilis, has been cloned on a bacterial plasmid pBR322. The size of the cloned segment was about M , = 8.0 x lo6. All of the genes for the four species of rRNA were linked on it, and there was a long spacer between the 5-S and 18-5 rRNA coding regions.The nucleotide sequences of the regions flanking the 5-S rRNA gene were determined and compared with those in the corresponding regions of

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Section: Discussionsupporting

confidence: 87%

Section: Sequences Were D(t-g-t-a-a-c-c-t) In T Utilis and D(t-a-t-cmentioning

confidence: 99%

Structure of the 5‐S Ribosomal RNA Gene and Its Adjacent Regions in Torulopsis utilis

Tabata

1980

European Journal of Biochemistry

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“…Ethylnitrosourea-resistant phosphodiester bonds in the S. cerevisiae ribosomal 5 S RNA-protein complex. The secondary structure is estimated according to the base-pairing scheme of Nishikawa and Takemura [25]. The solid triangles cover residues which are partially resistant to ethylnitrosourea modification in both the free and proteinassociated molecule; the solid circles cover residues which are partially resistant only in the protein-associated molecule.…”

Section: Resultsmentioning

confidence: 99%

Accessibility of phosphodiester bonds in the yeast ribosomal 5 S RNA protein complex

McDougall

Nazar

1986

FEBS Letters

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The tertiary structure of the protein-associated yeast ribosomal 5 S RNA was examined using ethylnitrosourea reactivity as a probe for phosphodiester bonds. A reduced reactivity was consistently observed in at least nine residues within four distinct regions of the RNA sequence. Seven of these were also observed in three regions of the free RNA molecule while two, A27 and Gw, were only present in the ribonucleoprotein complex. The results strongly suggest that the tertiary structure of the free eukaryotic 5 S RNA is largely conserved in the 5 S RNA-protein complex although it appears to be further stabilized in interaction with the ribosomal protein. S rRNARibosome Ribonucleoprotein Tertiary structure

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“…The stability of the resultant RNAs was assessed by labelling kinetics; stable RNAs are differentiated from significantly unstable molecules by the solid and broken arrows respectively. The consensus secondary structure is that of Nishikawa and Takemura (41). Conserved helical regions are shaded and identified by the numerals (I-V) above or below.…”

Section: Methodsmentioning

confidence: 99%

In vivoanalyses of the internal control region in the 5S rRNA gene fromSaccharomyces cerevisiae

et al. 1995

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The internal control region of the Saccharomyces cerevisiae 5S rRNA gene has been characterized in vivo by genomic DNase I footprinting and by mutational analyses using base substitutions, deletions or insertions. A high copy shuttle vector was used to efficiently express mutant 5S rRNA genes in vivo and isotope labelling kinetics were used to distinguish impeded gene expression from nascent RNA degradation. In contrast to mutational studies in reconstituted systems, the analyses describe promoter elements which closely resemble the three distinct sequence elements that have been observed In Xenopus laevis 5S rRNA. The results indicate a more highly conserved structure than previously reported with reconstituted systems and suggest that the saturated conditions which are used in reconstitution studies mask sequence dependence which may be physiologically significant. Footprint analyses support the extended region of protein interaction which has recently been observed In some reconstituted systems, but mutational analyses Indicate that these interactions are not sequence specific. Periodicity in the footprint provides further detail regarding the in vivo topology of the interacting protein.

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Nucleotide sequence of 5 S RNA from Torulopsis utilis

Cited by 138 publications

References 23 publications

Structure of the 5‐S Ribosomal RNA Gene and Its Adjacent Regions in Torulopsis utilis

Structure of the 5‐S Ribosomal RNA Gene and Its Adjacent Regions in Torulopsis utilis

Accessibility of phosphodiester bonds in the yeast ribosomal 5 S RNA protein complex

In vivoanalyses of the internal control region in the 5S rRNA gene fromSaccharomyces cerevisiae

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