Structure of the 5‐S Ribosomal RNA Gene and Its Adjacent Regions in Torulopsis utilis

Abstract

A DNA segment spanning one repeating unit of ribosomal RNA(rRNA) genes in a yeast strain, Tovulopsis utilis, has been cloned on a bacterial plasmid pBR322. The size of the cloned segment was about M , = 8.0 x lo6. All of the genes for the four species of rRNA were linked on it, and there was a long spacer between the 5-S and 18-5 rRNA coding regions.The nucleotide sequences of the regions flanking the 5-S rRNA gene were determined and compared with those in the corresponding regions of