Nucleotide sequence of a 3.5 kilobase fragment of malignant catarrhal fever virus strain WC11

Hsu, David S.; Shih, Ling-Na; Zee, Y. C.

doi:10.1007/bf01318352

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…Complete genomic sequences of three gammaherpesviruses were available: EBV, a herpesvirus of humans (4); herpesvirus saimiri (HVS), a herpesvirus of the New World monkey Saimiri sciureus (1); and equine herpesvirus 2 (EHV2) (51). Additional thymidine kinase (TK) gene sequences were obtained for alcelaphine herpesvirus 1 (25) and bovine herpesvirus 4 (31). Sequences for the major capsid protein genes of human herpesvirus 6B and human herpesvirus 7 were from Mukai et al (39).…”

Section: Methodsmentioning

confidence: 99%

Primary characterization of a herpesvirus agent associated with Kaposi's sarcomae

Moore

Gao

Dominguez

et al. 1996

J Virol

524

140

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Detection of novel DNA sequences in Kaposi's sarcoma (KS) and AIDS-related body cavity-based, non-Hodgkin's lymphomas suggests that these neoplasms are caused by a previously unidentified human herpesvirus. We have characterized this agent using a continuously infected B-lymphocyte cell line derived from an AIDS-related lymphoma and a genomic library made from a KS lesion. In this cell line, the agent has a large episomal genome with an electrophoretic mobility similar to that of 270-kb linear DNA markers during clamped homogeneous electric field gel electrophoresis. A 20.7-kb region of the genome has been completely sequenced, and within this region, 17 partial and complete open reading frames are present; all except one have sequence and positional homology to known gammaherpesvirus genes, including the major capsid protein and thymidine kinase genes. Phylogenetic analyses using both single genes and combined gene sets demonstrated that the agent is a gamma-2 herpesvirus (genus Rhadinovirus) and is the first member of this genus known to infect humans. Evidence for transient viral transmission from infected to uninfected cells is presented, but replication-competent virions have not been identified in infected cell lines. Sera from patients with KS have specific antibodies directed against antigens of infected cell lines, and these antibodies are generally absent in sera from patients with AIDS without KS. These studies define the agent as a new human herpesvirus provisionally assigned the descriptive name KS-associated herpesvirus; its formal designation is likely to be human herpesvirus 8.

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Section: Methodsmentioning

confidence: 99%

Primary characterization of a herpesvirus agent associated with Kaposi's sarcomae

Moore

Gao

Dominguez

et al. 1996

J Virol

524

140

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“…The molecular cloning of a genomic DNA fragment from the WC11 strain of the AHV-1 was previously reported by us [20]. The nucleotide sequence of 3389 base pairs of this virus was determined [4] and was used to develop a diagnostic method for detecting AHV-1 genome utilizing the reported polymerase chain reaction technology [18]. One pair of primers for PCR amplification was designed based on the virus DNA sequence.…”

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confidence: 99%

A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

Hsu

Shih

Castro

et al. 1990

Archives of Virology

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A sensitive diagnostic method specific for alcelaphine herpesvirus-1, causative agent of malignant catarrhal fever, has been developed. Based on the nucleotide sequence of the alcelaphine herpesvirus-1 genomic DNA, a pair of 30 nucleotide primers was selected and synthesized for detecting the virus genome using the polymerase chain reaction (PCR). The virus genome was detected in crude cell lysate using the amplification reaction.

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“…4,20,22 The only other reported AHV-1 sequence also has a substantial but slightly lower (57%) degree of sequence homology with the EBV genome. 11 The previous use of ruminant buffy coats to isolate infectious AHV-1 5,8 led to the search for a simple method of preparing bovine blood for PCR. Rapid hypoosmotic lysis of erythrocytes coupled with proteinase K, nonionic detergent, and heat treatments eliminated the need for lymphocyte purification and organic solvent extractions.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diagnosis of Alcelaphine Herpesvirus (Malignant Catarrhal Fever) Infections by Nested Amplification of Viral DNA in Bovine Blood Buffy Coat Specimens

1991

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A fragment of alcelaphine herpesvirus-1 (AHV-1; malignant catarrhal fever) DNA was subcloned into pUC 18 and sequenced. The subclone hybridized strongly to AHV-1 DNA, weakly to alcelaphine herpesvirus-2 (AHV-2) DNA, and not at all to DNA from bovine herpesvirus-1 (BHV-1; infectious bovine rhinotracheitis [IBR] virus), bovine herpesvirus-2 (BHV-2; bovine herpes mamillitis [BHM] virus), and bovine herpesvirus-4 (BHV-4; isolate DN599). A 2-stage (nested) polymerase chain reaction (PCR) diagnostic test was devised based on a portion of the subcloned AHV-1 DNA sequence. First and second stage amplified AHV-1 DNA targets were 487 and 172 base pairs (bp) in length, respectively. Unique Pvu II and Stu I restriction endonuclease cleavage sites confirmed the identity of amplified AHV-1 DNA. Five AHV-1 and 2 AHV-2 isolates were identically and specifically PCR positive. BHV-1, BHV-2, and BHV-4 viruses were negative by the same procedure. As little as 0.01 TCID50 AHV-1 was detected using the nested amplification procedure. Simple methods of buffy coat isolation from bovine blood were employed to prepare specimens for PCR. An AHV-1-infected calf was PCR positive from 3 to 77 days postinoculation (PI), with rising seroconversion first noted 14 days PI. The AHV-1 DNA sequence was 62% homologous to a portion of the Epstein-Barr virus genome. The nested PCR procedure may improve the viral diagnosis of clinical and subclinical alcelaphine herpesvirus infections.

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Nucleotide sequence of a 3.5 kilobase fragment of malignant catarrhal fever virus strain WC11

Cited by 5 publications

References 20 publications

Primary characterization of a herpesvirus agent associated with Kaposi's sarcomae

Primary characterization of a herpesvirus agent associated with Kaposi's sarcomae

A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

Molecular Diagnosis of Alcelaphine Herpesvirus (Malignant Catarrhal Fever) Infections by Nested Amplification of Viral DNA in Bovine Blood Buffy Coat Specimens

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