Nucleotide sequence of a ripening-related cDNA from avocado fruit

McGarvey, Douglas J.; Yu, Hong; Christoffersen, Rolf E.

doi:10.1007/bf00017736

Cited by 64 publications

(47 citation statements)

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“…The predicted amino acid sequence derived from the cDNA insert of pMELl was compared to EFE genes from tomato, ethl, eth2 ; previously designated gTOMA); from EFX cDNA from tomato-cell suspensions, pHTOM5 (Spanu et al, 1991); from EFE-related cDNA from avocado, pAVOe3 (McGarvey et al, 1990) and from carnation, pSR120 (Wang and Woodson, 1991) using the Ialign program of PIP(NBRF, Washington DC.). The Individual melons were placed in 2-1 containers that were sealed for 30 min.…”

Section: Comparison Of Predicted Amino-acid Sequencesmentioning

confidence: 99%

“…The predicted amino-acid sequences of pMELl and EFE or EFE-related genes from tomatoes (Holdsworth et al, , 1988Kock et al, 1991;Spanu et al, 1991), avocado (McGarvey et al, 1990) and carnation (Wang and Woodson, 1991) appear to be highly conserved with identity and similarity percentages of 74-81 % and 87-91 %, respectively. However, some deletions and extra-sequence insertions were observed essentially at their C-terminal ends.…”

Section: Comparison Of Predicted Amino-acid Sequencesmentioning

confidence: 99%

“…The identity of pTOMl3 was confirmed by the expression of ACC oxidase activity in pTOMl3-transformed Saccharomyces cerevisiue cells and Xenopus laevis oocytes (Spanu et al, 1991). cDNA with homology to pTOM13 have also been isolated from avocado (pAVOe3, McGarvey et al, 1990), apple fruit (pAP4, Ross et al, 1992; pAJ32, Dong et al, 1992) and senescent carnation flowers (pSR120, Wang and Woodson, 1991).The ability to extend the storage life and prevent spoilage by inhibiting ethylene biosynthesis in climacteric fruit has already been demonstrated in transgenic tomato plant expressing antisense EFE (Hamilton et al, 1990) and ACC synthase genes. In order to try and extend the storage life of melon fruit by inhibiting ethylene synthesis our initial aim was to isolate an EFE clone from a climacteric melon fruit cDNA library, using the heterologous pTOMl3…”

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Isolation of a ripening and wound‐induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene‐forming enzyme

Balagué

Watson

Turner

et al. 1993

European Journal of Biochemistry

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A cDNA clone (pMEL1) was isolated from a climacteric melon fruit cDNA library using the tomato ethylene-forming-enzyme (EFE) cDNA, pTOM13, as a probe. Northern analysis of RNA isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine the pattern of pMELl RNA expression. pMELl hybridized to a 1.3-kb transcript in climacteric fruit and wounded leaf tissue but was undetectable in preclimacteric fruit and unwounded leaves. Maximal expression of pMELl RNA occurred in wounded ripe fruit.pMELl contained a 1230-bp insert containing a predicted open reading frame of 318 amino acids and molecular mass of 35.3 kDa. The predicted amino acid sequence of pMELl was 73-81 % identical to the deduced amino acid sequences of tomato (pTOM13) EFE and EFE-related genes isolated from tomato and avocado fruit and senescent carnation petals. Genomic Southern analysis indicated that pMELl hybridized to a number of genomic fragments consistent with the presence of more than one pMEL1-related gene in melon. On Western analysis of total protein extracts from ripe tomato and melon fruit, using an antibody raised against tomato EFE (pTOM13) expressed in Escherichia coli, a single 35.5-kDa protein was detected. A 35-kDa product translated from in-vitro transcribed pMELl and immunoadsorbed by anti-EFE serum was very similar in size to the predicted 35.3-kDa pMELl cDNA protein product. These results indicate that pMELl may encode an EFE gene involved in ethylene biosynthesis during fruit ripening and may be identical to or share extensive sequence similarity with an EFE expressed in response to tissue wounding.Ethylene is an important plant growth regulator which is synthesized naturally during climacteric fruit ripening, leaf and flower senescence (Yang and Hoffmann, 1984). Ethylene also induces ripening and changes in gene expression when applied to mature unripe tomato fruit (Maunders et al., 1987) and accumulates in response to mechanical wounding (Boller and Kende, 1980) or pathogen infection of plant tissues (Yang and Hoffmann, 1984). The onset of ethylene production in ripening climacteric fruit is associated with the concomitant increase in the activities of both l-aminocyclopropane-1-carboxylate (ACC) synthase which converts Sadenosyl-t-methionine to ACC and ethylene-forming enzyme (EFE) which converts ACC to ethylene (Yang and Hoffmann, 1984). ACC-synthase genes have been isolated from several different plant species (Sat0 and Theologis, 1989;Nakajima et al., 1990;Van Der Straeten et al., 1990). ACC synthase is encoded by a divergent multigene family in tomato whose expression has been shown to be differentially Abbreviations. ACC, 1-aminocyclopropane-1-caxboxylic acid ; EFE, ethylene-forming enzyme, also known as ACC oxidase.Note. The nucleotide-sequence data published here has been submitted to the EMBL sequence data bank and is available under accession number X69935. regulated during fruit ripening and floral senescence (Rottmann et al., 1991). The gene encoding EFE ha...

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Section: Comparison Of Predicted Amino-acid Sequencesmentioning

confidence: 99%

Section: Comparison Of Predicted Amino-acid Sequencesmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 95%

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Isolation of a ripening and wound‐induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene‐forming enzyme

Balagué

Watson

Turner

et al. 1993

European Journal of Biochemistry

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“…A cDNA clone for tomato ACC oxidase has also been identified by expression in Xenopus laevis oocytes (Spanu et al, 1991). cDNA clones showing a high degree of nucleotide sequence homology to pTOM13 have also been isolated for apples (pAP4 [Ross et al, 19921 and pAel2 [Dong et al, 1992b]), avocados (pAVOe3 [McGarvey et al, 1990]), and camation flowers (pSR12O [Wang and Woodson, 19911). The importance of ethylene in climacteric fruit ripening has been clearly demonstrated by the retardation of the normal ripening process in the fruit of transgenic plants that express antisense RNA for ACC synthase or ACC oxidase (Hamilton et al, 1990) and show grossly reduced levels of ethylene synthesis.…”

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Apple Ripening-Related cDNA Clone pAP4 Confers Ethylene-Forming Ability in Transformed Saccharomyces cerevisiae

et al. 1993

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galactose allowed these cells to convert ACC to ethylene. l h e transformed yeast converted 1-amino-2-ethylcyclopropane-1-carboxylate isomers to I-butene with the same lR,2S-stereoseledivity as achieved by the native ACC oxidase from apples. Both ascorbate and Fez+ ions stimulated the rate of the production of ethylene from ACC by the transformed yeast, whereas Cu2+ and Co2+ were strongly inhibitory; these are features of ACC oxidase. Northern analysis of the total RNA from nontransformed and transformed yeast showed that the ability to convert the ACC to ethylene was correlated with the synthesis and accumulation of a nove1 1.2-kb mRNA that hybridized to the cDNA clone pAP4. We conclude that the cDNA sequence of the clone pAP4 encodes ACC oxidase.Ethylene is significantly important in many stages of plant development and growth. It is becoming increasingly apparent that the biosynthesis of ethylene is developmentally regulated and temporally activated in response to numerous biotic and abiotic factors. An increase in the leve1 of synthesis of this simple olefin occurs during leaf and flower peta1 senescence and the ripening of climacteric fruits and, in many tissues, following periods of temperature, wounding, or anaerobic stress (Yang and Hoffman, 1984). Ethylene is synthesized from Met via the intermediates S-adenosyl-L-methionine and ACC, which is converted to ethylene by the terminal biosynthetic enzyme ACC oxidase (Adams and Yang, 1979; Yang and Hoffman, 1984).

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Collagen Hydroxylases and the Protein Disulfide Isomerase Subunit of Prolyl 4‐Hydroxylases

Kivirikko

Pihlajaniemi

1998

Advances in Enzymology - And Related Areas of Molecular Biology

194

229

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Nucleotide sequence of a ripening-related cDNA from avocado fruit

Cited by 64 publications

References 10 publications

Isolation of a ripening and wound‐induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene‐forming enzyme

Isolation of a ripening and wound‐induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene‐forming enzyme

Apple Ripening-Related cDNA Clone pAP4 Confers Ethylene-Forming Ability in Transformed Saccharomyces cerevisiae

Collagen Hydroxylases and the Protein Disulfide Isomerase Subunit of Prolyl 4‐Hydroxylases

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