Nucleotide Sequence of Aminoglycoside 6'-N-Acetyltransferase (AAC(6')) Determinant from Serratia sp. 45.

Toriya, Minoru; Sakakibara, Masahiro; Matsushita, Kimiko; Morohoshi, Toshiro

doi:10.1248/cpb.40.2473

Cited by 11 publications

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“…The complete aacA4 gene cassette is 637 or 638 bp long and has been shown to be mobile (4). However, the first 22 bases of the aacA4 cassette are not present in the sequence reported by Toriya et al (41). In this study, part of the region between the 5Ј-conserved segment and the aacA4 coding region was found to be between 64.9 and 84.9% identical to the first half of the catB1, catB2, and catB3 genes and of the Ps open reading frame.…”

Section: Discussioncontrasting

confidence: 67%

“…5). Examination of the complete sequence of Toriya et al (41) revealed that this region is located between the integron 5Ј-conserved segment and an aacA4 gene and appears to represent the 5Ј half of an integron-associated catB cassette that has become fused to an aacA4 gene cassette (see Discussion). A polypeptide predicted from an open reading frame contained in this region is also closely related to the N-terminal half of the CATB proteins (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…An incomplete catB gene cassette was found in the published sequence of a region containing an aacA4 gene isolated from an Ak r Serratia sp. 45 isolate (41). Though the authors did not note this, the sequence consists of the last 253 bases of an integron 5Ј-conserved segment (bases Ϫ578 to Ϫ326 in reference 41) separated by 325 bases (bases Ϫ325 to Ϫ1) from an aacA4 gene (bases 1 to 555).…”

Section: Discussionmentioning

confidence: 99%

“…6a. Whether the 3Ј end of the aacA4 cassette is present is [39]), catB2 (accession number M80188 [31]), catB3 (accession number U13880 [this study]), and catB4⌬1 (accession number S49888 [41]). All accession numbers refer to the EMBL and GenBank databases.…”

Section: Discussionmentioning

confidence: 99%

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New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

Bunny

Hall

Stokes

1995

Antimicrob Agents Chemother

117

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The multidrug resistance plasmid pBWH301 was shown to contain a sulI-associated integron with five inserted gene cassettes, aacA7-catB3-aadB-oxa2-orfD, all of which can be mobilized by the integron-encoded DNA integrase. The aadB, oxa2, and orfD cassettes are identical to known cassettes. The aacA7 gene encodes a protein that is a member of one of the three known families of aminoglycoside acetyltransferases classified as AAC(6)-I. The chloramphenicol acetyltransferase encoded by the catB3 gene is closely related to members of a recently identified family of chloramphenicol acetyltransferases. The catB3 gene displays a relatively high degree of sequence identity to a chromosomally located open reading frame in Pseudomonas aeruginosa, and this may represent evidence for the acquisition by a cassette of a chromosomal gene.pBWH301 is a 69-kb multidrug-resistant conjugative plasmid isolated from two bacterial species, Enterobacter aerogenes and Enterobacter cloacae, during an outbreak of amikacin resistance in Hospital Vargas in Venezuela (19). Most of the drug resistance genes in pBWH301 were found clustered within a 6.0-kb BamHI fragment that confers resistance to the aminoglycosides amikacin (Ak . Two aminoglycoside resistance genes were identified on the basis of aminoglycoside resistance profiles: an aacA gene encoding a type I aminoglycoside acetyltransferase [AAC(6Ј)-I] that confers resistance to amikacin, netilmicin, and tobramycin and an aadB gene encoding an aminoglycoside adenylyltransferase [AAD(2Љ)] that confers resistance to gentamicin, kanamycin, and tobramycin (19). A chloramphenicol resistance gene and a sulfonamide resistance gene were also identified. The presence of the restriction sites characteristic of the sulI gene, which is normally found in the 3Ј-conserved segment of sulI-associated integrons (37), suggested that the resistance genes may be integron associated (19).The ability of bacteria to develop multiple-drug resistance is due in part to their ability to acquire new antibiotic resistance genes. Mobile elements called integrons determine a site-specific recombination system that is responsible for the acquisition of many antibiotic resistance determinants (14,15,37). A large number of antibiotic resistance genes (conferring resistance to aminoglycosides, ␤-lactams, chloramphenicol, and trimethoprim), as well as several unidentified open reading frames, have been found as inserts in integrons (13). These genes are contained in individual mobile units called gene cassettes that can be inserted into and excised from an integron by site-specific recombination (3-5, 13). Gene cassettes consist of a gene coding region (or open reading frame) and a recombination site known as a 59-base element which is located 3Ј to the gene in the linear integrated form (4,13,14). The 59-base elements vary in sequence and length but are all imperfect inverted repeats and are related to a consensus sequence at their outer ends (1, 5, 13). The 59-base elements play an essential role in the process of gene acqui...

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Section: Discussioncontrasting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

Bunny

Hall

Stokes

1995

Antimicrob Agents Chemother

117

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“…The aac(6Ј)-IbЈ sequence of the fused cassette encoded a 6Ј-N-aminoglycoside acetyltransferase that was characterized by a Leu-1193Ser substitution [numbering of the reference aac(6Ј)-Ib sequence (42)], and differed by three amino acid substitutions-Val-183Leu, Leu-423Val, and Ser1003Gly-from the already-described aac(6Ј)-IbЈ genes (5,17,31,32,41). The amino acid at position 119 has been found to be critical functionally in that a Leu-to-Ser switch at this position was responsible for the loss of amikacin resistance conferred by the aac(6Ј)-Ib gene and the acquisition of gentamicin resistance, conveyed by the aac(6Ј)-IbЈ gene (17).…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of a Novel Class 1 Integron Containing bla _GES-1 and a Fused Product of aac(3)-Ib/aac(6 " )-Ib " Gene Cassettes in Pseudomonas aeruginosa

Dubois

Poirel

Marie

et al. 2002

Antimicrob Agents Chemother

130

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As seen by the disk diffusion method, the clinical strain of Pseudomonas aeruginosa Pa695, resistant to all extended-spectrum cephalosporins and aminoglycosides, exhibited an unusual synergistic effect between ceftazidime and imipenem. This isolate produced an extended-spectrum ␤-lactamase (ESBL) with a pI of 5.8 that appeared to be chromosomally encoded. Cloning experiments revealed that this ESBL was encoded by bla GES-1 , previously described in an integron from Klebsiella pneumoniae. In P. aeruginosa Pa695, a higher level of resistance to ceftazidime than to ticarcillin was observed, and no synergy between the ␤-lactamase inhibitors and extended-spectrum cephalosporins was detected, in contrast to the resistance pattern observed in K. pneumoniae. Further sequence analysis demonstrated that the bla GES-1 gene cassette was located in a class 1 integron, which contained another sequence corresponding to the fused aac(3)-Ib and aac(6)-Ib gene cassettes. The fusion product was functional, as was the product of each gene cloned separately: AAC(3)-I, despite the deletion of the four last amino acids, and AAC(6), which carried three amino acid changes compared with the most homologous sequence. The AAC(3)-I protein conferred an expected gentamicin and fortimicin resistance, and the AAC(6), despite the Leu-1193Ser substitution, yielded resistance to kanamycin, tobramycin, and dibekacin, but slightly affected netilmicin and amikacin, and had no apparent effect on gentamicin. The fusion product conveyed a large profile of resistance, combining the AAC(6) activity with a higher level of gentamicin resistance without accompanying fortimicin resistance.

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A spontaneous point mutation i the aac(6′)-lb′ gene results in altered substrate specificity of aminoglycoside 6′-N-acetyltransferase of a Pseudomonas fluorescens strain

Lambert¹

1994

FEMS Microbiology Letters

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The aac(6')-Ib' gene from Pseudomonas fluorescens BM2687, encoding an aminoglycoside 6'-N-acetyltransferase type II which confers resistance to gentamicin but not to amikacin, was characterized. Nucleotide sequence determination indicated total identity between aac(6')-Ib' and the aac(6')-Ib gene from Pseudomonas aeruginosa BM2656 [1] with the exception of a C-to-T transition that results in a serine to leucine substitution at position 83 of the deduced polypeptide. The aac(6')-Ib gene specifies a type I enzyme which confers resistance to amikacin but not to gentamicin [2]. It thus appears that the point mutation detected is responsible for enzymic altered substrate specificity.

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Nucleotide Sequence of Aminoglycoside 6'-N-Acetyltransferase (AAC(6')) Determinant from Serratia sp. 45.

Cited by 11 publications

References 0 publications

New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

Molecular Characterization of a Novel Class 1 Integron Containing bla _GES-1 and a Fused Product of aac(3)-Ib/aac(6 " )-Ib " Gene Cassettes in Pseudomonas aeruginosa

A spontaneous point mutation i the aac(6′)-lb′ gene results in altered substrate specificity of aminoglycoside 6′-N-acetyltransferase of a Pseudomonas fluorescens strain

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Nucleotide Sequence of Aminoglycoside 6'-N-Acetyltransferase (AAC(6')) Determinant from Serratia sp. 45.

Cited by 11 publications

References 0 publications

New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

Molecular Characterization of a Novel Class 1 Integron Containing bla GES-1 and a Fused Product of aac(3)-Ib/aac(6 " )-Ib " Gene Cassettes in Pseudomonas aeruginosa

A spontaneous point mutation i the aac(6′)-lb′ gene results in altered substrate specificity of aminoglycoside 6′-N-acetyltransferase of a Pseudomonas fluorescens strain

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Molecular Characterization of a Novel Class 1 Integron Containing bla _GES-1 and a Fused Product of aac(3)-Ib/aac(6 " )-Ib " Gene Cassettes in Pseudomonas aeruginosa