Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411

Ishiguro, Naotaka; Sato, Gihei

doi:10.1128/jb.170.4.1902-1906.1988

Cited by 40 publications

(25 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sequence analysis of IS911 indicates that it is a member of the IS3 group of elements (32,33). This analysis also revealed that IS3411 (12), IS476 (14), and ISRJ (29) belong to this group. The disposition of open reading frames and possible control of expression of transposition functions of IS911 and other members of the group are also discussed.…”

mentioning

confidence: 90%

See 1 more Smart Citation

Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences

1990

View full text Add to dashboard Cite

Twenty-nine clear-plaque mutants of bacteriophage k were isolated from a ShigeUa dysenteriae lysogen. Three were associated with insertions in the cI gene: two were due to insertion of IS600, and the third resulted from insertion of a new element, IS911. IS911 is 1,250 base pairs (bp) long, carries 27-bp imperfect terminal inverted repeats, and generates 3-bp duplications of the target DNA on insertion. It was found in various copy numbers in all four species of Shigella tested and in Escherichia coli K-12 but not in E. coli W. Analysis of IS911-mediated cointegrate molecules indicated that the majority were generated without duplication of IS911.They appeared to result from direct insertion via one end of the element and the neighboring region of DNA, which resembles a terminal inverted repeat of IS911. Nucleotide sequence analysis revealed that IS911 carries two consecutive open reading frames which code for potential proteins showing similarities to those of the IS3 group of elements.In contrast to many strains of Escherichia coli, the genome of members of the closely related genus Shigella carries relatively large numbers of a variety of insertion sequences. It has been estimated, for example, that the four Shigella species carry 40 to 200 copies of insertion sequences closely related to IS] (27). This elevated number of IS-like sequences is not restricted to ISI; it also includes IS2 and IS4 (15; Fayet and Prere, unpublished observations; see reference 11 for review).In order to assess transposition activity from the Shigella dysenteriae genome, we used a simple technique described by Lieb (16) which employs bacteriophage A cI ind as a trap for insertion sequences. By screening for clear-plaque mutants among phages released spontaneously from an E. coli lysogen, it was determined that of 25 mutants, 15 were the result of insertion of IS elements into the cI gene; of these, 7 insertions were of IS], 5 were of IS5, and 3 were of IS3.Since some strains of S. dysenteriae are sensitive to lambdoid phages, we used this technique to obtain some indication of the relative activity of the various IS elements carried by the S. dysenteriae chromosome. We found that the spectrum of cI mutants obtained in S. dysenteriae is very different from that observed in E. coli. No example of an ISl insertion was found, and only 3 of the 29 independent mutants were found to carry insertions in the cI gene. This result suggests that the chromosomal copies of IS] in S. dysenteriae may be less active than those of E. coli. Of the three insertions obtained, two were examples of IS600, an element previously isolated by physical means from the Shigella sonnei chromosome (21), while the third (IS911) proved to be an element not described previously.We present here the nucleotide sequence of IS911 (included in the European Molecular Biology Laboratory sequence data base under accession number X17613), assess its distribution within E. coli K-12, E. coli W, and the four species of Shigella, and analyze some of its transposition properties.

show abstract

mentioning

confidence: 90%

“…2 (33), and IS861 (32). Three additional elements, IS3411 (12), ISRJ (29), and IS476 (14), are also members of this group (see below). They exhibit a limited degree of similarity at the nucleotide sequence level (33) (data not shown).…”

Section: Methodsmentioning

confidence: 99%

Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences

1990

View full text Add to dashboard Cite

show abstract

“…This is the first reported case of which we are aware, however, of insertional inactivation of a gene by IS3411. IS3411 has been shown to constitute the ends of the composite transposon Tn3411, which encodes citrate utilization genes (16)(17)(18) (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

“…Shown is the inverse complement of the actual sequence obtained. 17). The generation of cointegrates is believed to be evidence of replicative transposition.…”

Section: Methodsmentioning

confidence: 99%

Insertional inactivation of an Escherichia coli urease gene by IS3411

Collins

Gutman

1992

J Bacteriol

View full text Add to dashboard Cite

Ureolytic Escherichia coli are unusual clinical isolates that are found at various extraintestinal sites of infection, predominantly the urinary tract. The urease-positive phenotype is unstable in approximately 25% of these isolates, and urease-negative segregants are produced at a high frequency. We have studied the nature of the urease-positive-to-negative transition in one of these isolates, designated E. coli 1021. Southern hybridization experiments with genomic DNA extracted from seven independent E. coli 1021 urease-negative segregants revealed the presence of a 1.3-kb DNA insertion in the urease gene cluster. A DNA fragment containing the DNA insertion was cloned from one of the urease-negative segregants. This cloned DNA fragment was capable of mediating cointegrate formation with the conjugative plasmid pOX38, suggesting that the DNA insertion was a transposable element. The insert was identified as an IS3411 element in ureG by DNA sequence analysis. A 3-bp target duplication (CTG) flanking the insertion element was found. DNA spanning the insertion site was amplified from the other six urease-negative segregants by using the polymerase chain reaction. The DNA sequence of the amplified fragments indicated that an IS3411 element was found in an identical site in all urease-negative segregants examined. These data suggest that in E. coli 1021, IS3411 transposes at a high frequency into ureG at a CTG site, disrupting this gene and eliminating urease activity.

show abstract

“…The genes for citrate utilization are present in widely distributed plasmids (166). In some cases the genes reside in a transposon, Tn3411, flanked by two copies of IS3411 (167). Similarly, lac genes are carried by many plasmids, converting essentially lac-less bacterial species to lac+ (59,121,285); in some cases the lac genes reside in a transposon structure, as in Tn951 (59).…”

Section: Integrity Of Bacterial Species Versus Lateral Transfermentioning

confidence: 99%

Organization of the Bacterial Chromosome

Schumann

2006

Dynamics of the Bacterial Chromosome

View full text Add to dashboard Cite

Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411

Cited by 40 publications

References 21 publications

Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences

Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences

Insertional inactivation of an Escherichia coli urease gene by IS3411

Organization of the Bacterial Chromosome

Contact Info

Product

Resources

About