Nucleotide sequence of the 3′-terminal region of citrus tatter leaf virus RNA

Abstract

The 3'-terminal sequence of citrus tatter leaf virus lily isolate (CTLV-L) was determined from cloned cDNA. The sequence contains two open reading frames (ORFs). ORF1 encodes a protein that contains consensus sequences associated with the RNA-dependent RNA polymerase. ORF2, which is in a different reading frame within ORF1, can encode a 36 kD protein, putatively identified as a movement protein. CTLV-L coat protein (CP) was found to be located in the C-terminal region of the polyprotein encoded by ORF1. Evolutionary relationships and classification of capilloviruses is discussed.

Cherry virus A: cDNA cloning of dsRNA, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry

“…3 is limited to a partial alignment including the type members of the two genera, ASGV and ACLSV (for genome organization see Fig. 1), respectively and CTLV, which was reported to be a closely related strain of ASGV (Ohira et al, 1994). The Arg and Asp residues conserved in rod-shaped viruses and putatively involved in salt bridge formation (Dolja et al, 1991) are in bold type.…”

“…The ASGV particles contain an ssRNA of 6496 nucleotides, excluding the poly(A) tail, and a coat protein of 27 kDa (Yoshikawa et al, 1992). The only other definitive member is citrus tatter leaf virus (CTLV), but recent data suggest that CTLV should be regarded as a closely related strain of ASGV rather than as a different virus (Ohira et al, 1994). Capilloviruses have morphologically comparable particles to the newly established genus Trichovirus of which apple chlorotic * Fax +49 6221 861222. e-mail ba69ed@genius.embnet.dkfzheidelberg.de…”

Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus: comparative sequence analysis of capillovirus genomes

“…The nucleotide sequence and genome organization of the 3' terminus of CTLV-L RNA (Yoshikawa et al, 1993;Ohira et al, 1994) displayed a strong similarity to that of ASGV. In this paper we report the complete sequence and genome organization of CTLV-L, compare it with the genomes of other related viruses, and describe the production of infectious transcripts of CTLV-L from a full-length clone of the genome.…”

“…CTLV-L RNA was prepared as described previously (Ohira et al, 1994). cDNA was synthesized from CTLV-L RNA using the cDNA synthesis system Plus (Amersham) with NotI tagged oligo(dT) 5' ATGCGG-CCGC(dT)I 8 3' as primer, cDNAs larger than 3 kbp were recovered by low melting point agarose gel electrophoresis, inserted into the EcoRV site of pBluescriptII SK(-) (Stratagene) and cloned in Escherichia coli XL1-Blue.…”

“…The region of ORF1 that contains ORF2 is not conserved between CTLV and ASGV; only 54 % identity exists in ORF1 amino acids 1583-1851. This suggests that these regions encode virus-specific proteins (such as proteins determining host range) rather than conserved proteins (such as proteins essential for virus replication), or that there are less severe evolutionary constraints than on the rest of the viral proteins (Ohira et al, 1994). If the latter is the case, it could be speculated that ACLSV or PVT evolved from capilloviruses by losing this dispensable region altogether.…”