Nucleotide sequence of the coat protein gene of canine parvovirus

Abstract

The nucleotide sequence of the canine parvovirus (CPV2) from map units 33 to 95 has been determined. This includes the entire coat protein gene and noncoding sequences at the 3' end of the gene, exclusive of the terminal inverted repeat. The predicted capsid protein structures are discussed and compared with those of the rodent parvoviruses H-1 and MVM.

Emergence, Natural History, and Variation of Canine, Mink, and Feline Parvoviruses

“…Alternative splices predicted to generate VP-1 and VP-2 messages are most likely derived from two different splice donors. One, prior to the methionine codon thought to be the initiation codon of VP-1, would give rise to an mRNA in which the first AUG used would be that initiating VP-2 (Rhode, 1985, Carlson et al, 1985Morgan and Ward, 1986). The alternatively spliced mRNA would most likely use a predicted splice donor after the predicted ATG initiating VP-1, giving 10 codons prior to the splice, then the mRNA could be spliced to the same acceptor as for the VP-2 message.…”

Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease

“…Comparison with the previously published sequence of CPV-b strain [32] showed a high rate of point mutations and one deletion which could have been generated by cell culture passages.…”

“…This result emphasises the high variability of the CPV genome and sequence comparison was further investigated on three other CPV strains [27,30,32] and one FPLV strain [20]. 22 point mutations were observed between the different CPV sequences and 15 between CPV-b 108 and FPLV (Table 1).…”

“…Comparison of this sequence with that of three other CPV strains [27,30,32] and one FPLV strain [20] revealed point mutations between the different isolates (Table 1). Furthermore, a 15 nucleotides deletion, located in the NS 1 gene, and extending from nucleotide 2040 to nucleotide 2056 of the Norden CPVs strain (CPV-N) sequence [30] was observed in the cloned DNA.…”

“…Cells used for virus propagation were freshly seeded Crandell feline kidney cells maintained with Eagles's minimum essential medium supplemented with 10% foetal bovine serum. The CPV strain used in the present study was derived from the Carmichael strain (CPVb) partially sequenced at passage 88 [8,32]. It was obtained from a commercial vaccine at passage 108 and six additional passages in cell culture were performed before DNA cloning.…”