Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA

Lusby, Edward W.; Fife, Kenneth H.; Berns, Kenneth I.

doi:10.1128/jvi.34.2.402-409.1980

Cited by 199 publications

(97 citation statements)

References 26 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The nucleotide sequence as well as the length of the palindromes at each end of a given genome was found to be unrelated for most of the parvoviruses analysed so far, the 3'-terminal palindrome being between 115 and 125, the 5'-terminal palindrome between 220 and 240 nucleotides long [4]. However, the terminal 145 nucleotides of the AAV type 2 (AAV-2) genome are identical, being arranged as inverted repeats [6]. Recently published data from Shade et al [7] indicate that the genome of the autonomous parvovirus B19 also has inverted terminal repeats.…”

Section: Structure and Organization Of The Genomementioning

confidence: 99%

Parvoviruses: agents of distinct pathogenic and molecular potential

Siegl

Tratschin

1987

FEMS Microbiology Letters

View full text Add to dashboard Cite

Parvoviruses are small, spherical and extremely stable particles with a genome of linear single‐stranded DNA. They are widespread in nature and thus have been in virtually every animal species and in man. Many parvoviruses were initially isolated by chance and could not be associated with clinical disease. Today, however, a considerable number of these agents are known to cause a broad spectrum of syndromes including fetal death, malformations, dwarfism, cerebellar hypolasia and ataxia, enteritis, panleukopenia, aplastic anemia, and immunologic disorders. Biological, pathological, and molecular studies revealed that all these syndromes are explainable on the basis of two predominant requirements for productive cytolytic parvovirus replication: the availability of cellular helper function(s) provided exclusively by mitotically active cells in the phase of cellular DNA replication, and by apparently specific, yet so far undefined cellular functions

show abstract

Section: Structure and Organization Of The Genomementioning

confidence: 99%

Parvoviruses: agents of distinct pathogenic and molecular potential

Siegl

Tratschin

1987

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…The viral genome consists of a linear DNA molecule with inverted terminal repeats (145 base pairs [bp]). The first 125 bp of the terminal repeat is a complex palindrome which can exist in either of two orientations (30). Genetic analysis of AAV has revealed at least four regions of the genome with distinct phenotypes (see Fig.…”

mentioning

confidence: 99%

Adeno-associated virus general transduction vectors: analysis of proviral structures

McLaughlin

Collis

Hermonat

et al. 1988

J Virol

307

120

View full text Add to dashboard Cite

We used two kinds of adeno-associated virus (AAV) vectors to transduce the neomycin resistance gene into human cells. The first of these (d152-91) retains the AAV rep genes; the second (d13-94) retains only the AAV terminal repeats and the AAV polyadenylation signal (428 base pairs). Both vectors could be packaged into AAV virions and produced proviral structures that were essentially the same. Thus, the AAV sequences that are required in cis for packaging (pac), integration (int), rescue (res), and replication (ori) of viral DNA are located within a 284-base-pair sequence that includes the terminal repeat. Most of the G418r cell lines (73%)

show abstract

“…AAV is so small that it only encodes two genes; only one of which, replication (Rep) is required for viral replication [44]. These genes are flanked by identical inverted terminal repeats (ITRs) of 146 nts that form T-shaped hairpins, due to three palindromic sequences residing within the ITRs [53]. The ITRs are the only cis-acting sequences necessary for viral replication [54], which enhanced its development as a vector for gene delivery.…”

Section: Aav Replication and Integrationmentioning

confidence: 99%

RAD52: Viral Friend or Foe?

Hendrickson

2020

Cancers

View full text Add to dashboard Cite

Mammalian Radiation Sensitive 52 (RAD52) is a gene whose scientific reputation has recently seen a strong resurgence. In the past decade, RAD52, which was thought to be dispensable for most DNA repair and recombination reactions in mammals, has been shown to be important for a bevy of DNA metabolic pathways. One of these processes is termed break-induced replication (BIR), a mechanism that can be used to re-start broken replication forks and to elongate the ends of chromosomes in telomerase-negative cells. Viruses have historically evolved a myriad of mechanisms in which they either conscript cellular factors or, more frequently, inactivate them as a means to enable their own replication and survival. Recent data suggests that Adeno-Associated Virus (AAV) may replicate its DNA in a BIR-like fashion and/or utilize RAD52 to facilitate viral transduction and, as such, likely conscripts/requires the host RAD52 protein to promote its perpetuation.Cancers 2020, 12, 399 2 of 11 genome integrity and, because of this, these cells now show increased sensitivity to chemo [9,10] and synthetic-lethality-based therapies [11][12][13]. Importantly, there is no evidence to suggest that RAD52 plays any role in C-NHEJ.

show abstract

Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA

Cited by 199 publications

References 26 publications

Parvoviruses: agents of distinct pathogenic and molecular potential

Parvoviruses: agents of distinct pathogenic and molecular potential

Adeno-associated virus general transduction vectors: analysis of proviral structures

RAD52: Viral Friend or Foe?

Contact Info

Product

Resources

About