Nucleotide sequence of the iron regulatory gene fur

Abstract

The fur gene of Escherichia coli is involved in all iron-regulated transcriptions hitherto studied. The nucleotide sequence of an 868 basepair fragment containing the fur gene was determined. There was only a single longer reading frame. The amino acid sequence derived from the nucleotide sequence comprised 148 amino acids that together made a polypeptide of 16,795 daltons. The amino acid sequence was confirmed by determination of the amino acid composition, the carboxy-terminal lysine residue and the internal Lys-Lys and Lys-Arg sequences of the isolated Fur protein. The nucleotide sequence contains typical initiation and termination sites for transcription and translation.

Fur (ferric uptake regulation) protein and CAP (catabolite‐activator protein) modulate transcription of fur gene in Escherichia coli

“…pABN203 plasmid was digested to completion with restriction endonuclease HpuII. This digestion affords a 269-bp fragment, which according to the published sequence of the fur gene [9] extends over 185 bp upstream of the start of the structural gene and 84 bp (equivalent to 28 amino acids) of the structural gene. This fragment was purified and the overhanging HpaII extremes filled-in with the Klenow fragment of DNA polymerase I in the presence of an excess of dGTP and dCTP [16].…”

“…This gene was first defined genetically [6,7] as a negative regulator of iron-controlled genes mapping at min 15.7 of the E. coli genetic map [6]. The fur gene of E. coli K12 was subsequently cloned [8] and sequenced [9] and its protein product (17 kDa) was purified to homogeneity (Wee et al unpublished results).…”

Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants

“…Fur homologs have been reported for at least 13 species of gram-negative bacteria, including Escherichia coli (13,25), Salmonella typhimurium (9), Yersinia pestis (27, 28), Vibrio anguillarum ( By analogy to other bacterial species, we hypothesized the involvement of a Fur homolog in the regulation of iron acquisition systems in Bordetella species. Manganese-resistant mutants of B. bronchiseptica B013N (2) were selected on LuriaBertani agar plates containing 10 mM MnCl 2 essentially as described by Hantke (14) for the selection of fur mutants of E. coli K-12.…”

“…Fur homologs have been reported for at least 13 species of gram-negative bacteria, including Escherichia coli (13,25), Salmonella typhimurium (9), Yersinia pestis (27,28), Vibrio anguillarum (31), Vibrio vulnificus (21), Vibrio cholerae (20), Klebsiella pneumoniae (33), Pseudomonas aeruginosa (23), Neisseria gonorrhoeae (5), Neisseria meningitidis (17,30), Legionella pneumophila (16), Campylobacter jejuni (35), and B. pertussis (4; also, this study).…”

Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor

“…This positive regulation is mediated by the Fur protein [12,27] but so far a Fur homologue has not been described in H. parasuis yet. Interestingly, and in contrast with other iron uptake mechanisms, such as transferrin binding proteins [2,29], FhuA expression in H. parasuis was constitutive.…”