Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis.

Devare, S G; Reddy, E. Premkumar; Law, Jean; Robbins, K C; Aaronson, Stuart A.

doi:10.1073/pnas.80.3.731

Cited by 235 publications

(59 citation statements)

References 40 publications

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“…Using the FMD -SIV relationship, all seven picornaviral VP2s could then be aligned with all seven retroviral p24s ( Figure 5). Within the PIR database there remained only one other retroviral gag family exemplified by simian sarcoma virus (Devare et al, 1983). Only about half of its p24 sequence could be confidently aligned with the others; it is not shown in Figure 5.…”

Section: Coc Atcrfytldsvqwqkts--p---gwwwklpdalsnl-----------glfgqnmqymentioning

confidence: 99%

A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites.

Argos¹

1989

The EMBO Journal

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With the use of a sensitive sequence comparison algorithm, a homology has been suggested between the primary structures of simian immunodeficiency virus (SIV) p24 core protein and foot‐and‐mouth disease virus (FMD) VP2 coat protein. Since the FMD sequence is homologous to picornaviral VP2 sequences with known three‐dimensional architecture and since the SIV p24 sequence can be convincingly aligned with that from human immunodeficiency virus (HIV), it was possible to predict an eight‐stranded beta‐barrel fold for the HIV core protein. From analogy with the known environments of the picornaviral coats, p24 sequence spans could be predicted as likely candidates for antibody attachment. These suggestions may be important for development of an AIDS vaccine.

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Section: Coc Atcrfytldsvqwqkts--p---gwwwklpdalsnl-----------glfgqnmqymentioning

confidence: 99%

A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites.

Argos¹

1989

The EMBO Journal

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“…A viral oncogene v-SLS clone p-v-sis was obtained from the Japanese Cancer Research Resources Bank. A 1.3-kb PstI fragment of p-v-sis was used (7). The INSR gene, SODl gene, and c-sis gene were mapped to chromosome 19~13.3-p13.2, 21q22.1, and 22q12.3-13.1, respectively (1,17,25).…”

Section: Analysis Of Giant Dna Fragments From Sorted Chromosome By Pumentioning

confidence: 99%

Isolation of giant DNA fragments from flow‐sorted human chromosomes

et al. 1990

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We have established a method using a conventional cell sorter equipped with a single argon laser to sort intact human chromosomes that can be used as a source for the production of giant DNA fragments. Various improvements were made to both the equipment and sorting method to enhance the sorting resolution and avoid destruction of chromosomal DNA. Using this improved method chromosomes 21 and 22 were sorted from the B-lymphoblastoid line GM00130B, digested with the rare cutting restriction endonuclease NotI, and analyzed by pulsed field gel electrophoresis followed by Southern hybridization using the AZu repetitive sequence as a probe. More than 25 discrete NotI giant DNA fragments ranging from 50 kb to longer than 2.5 Mb were separated and the size distribution pattern was unique for each chromosome, indicating successful sorting of intact chromosomes. The cumulative size of these AZu-positive NotI DNA fragments were 22.7 Mb and 25.5 Mb for chromosomes 21 and 22, respectively. These values are 47% and 49% of the estimated size of chromosomes 21 (48 Mb) and 22 (52 Mb).

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“…The v-sis oncogene, isolated from a woolly monkey sarcoma as the oncogene of simian sarcoma virus (5,27), arose as the result of a recombination event between simian sarcoma-associated virus and normal genetic information. Nucleotide sequence analysis of the v-sis and c-sis genes has shown that they encode the B chain of platelet-derived growth factor (PDGF) (6,20,30).…”

mentioning

confidence: 99%

“…Nucleotide sequence analysis of the v-sis and c-sis genes has shown that they encode the B chain of platelet-derived growth factor (PDGF) (6,20,30). The v-sis oncogene contains an env-sis fused open reading frame of 813 nucleotides, potentially encoding a protein of 33 kilodaltons (kDa) (5). The v-sis gene product has been identified in simian sarcoma virus-normal rat kidney-transformed cells as a PDGF-related protein of 28 kDa (5,24), termed p28sis, and PDGF-related proteins of 20 (8) and 17 (23) kDa have been detected in the medium of simian sarcoma virus-normal rabbit kidneytransformed cells.…”

mentioning

confidence: 99%

“…The v-sis oncogene contains an env-sis fused open reading frame of 813 nucleotides, potentially encoding a protein of 33 kilodaltons (kDa) (5). The v-sis gene product has been identified in simian sarcoma virus-normal rat kidney-transformed cells as a PDGF-related protein of 28 kDa (5,24), termed p28sis, and PDGF-related proteins of 20 (8) and 17 (23) kDa have been detected in the medium of simian sarcoma virus-normal rabbit kidneytransformed cells. The conditioned media from simian sarcoma virus-transformed cells is able to cause autophosphorylation of the cell surface PDGF receptor, lending support to the autocrine model of transformation (4,8,15,(23)(24)(25).…”

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Biosynthesis of the v-sis gene product: signal sequence cleavage, glycosylation, and proteolytic processing.

Hannink¹,

Donoghue²

1986

Mol. Cell. Biol.

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The v-sis oncogene and its cellular homolog c-sis encode chain B of platelet-derived growth factor. Cells transformed by v-sis produce a platelet-derived growth factor-related molecule which is able to stimulate the platelet-derived growth factor receptor in an autocrine fashion. Site-directed mutagenesis was used to construct several mutations which substitute charged residues for hydrophobic residues in the proposed signal sequence of the v-sis gene product. Two of these mutations resulted in the synthesis of altered v-sis gene products with an unexpected nuclear location and a loss of biological activity. We also report here the intracellular localization of the v-sis gene product to the endoplasmic reticulum-Golgi compartment, where signal sequence cleavage and N-linked glycosylation occur. The v-sis gene product contains no transmembrane regions, as it is completely protected within isolated microsomes from trypsin proteolysis. Site-directed mutagenesis was also used to alter a proposed proteolytic processing site in the v-sis gene product. This mutant v-sis gene, which encodes Asn-Ser in place of Lys-Arg at residues 110 to 111, was found to retain full biological activity.

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Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis.

Abstract: The complete nucleotide sequence ofthe proviral genome of simian sarcoma virus (SSV), an acute transforming retrovirus of primate origin, has been determined. Like

Cited by 235 publications

References 40 publications

A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites.

A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites.

Isolation of giant DNA fragments from flow‐sorted human chromosomes

Biosynthesis of the v-sis gene product: signal sequence cleavage, glycosylation, and proteolytic processing.

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