Nucleotide sequences of cytosolic 5S ribosomal RNAs from two gymnosperms,<i>Gnetum gnemon</i>and<i>Ephedra kokanica</i>

Melekhovets, Yu.F.; Troitsky, A. V.; Valiejo-Roman, K. M.; Bobrova, V. K.; Antonov, A. S.

doi:10.1093/nar/16.9.4155

Cited by 7 publications

(2 citation statements)

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“…The 5S rRNA was encoded by the same DNA strand as the 35S genes (direct orientation). The genic region of the first copy (5S1) was 100% identical to the sequence of 5S rRNA from E. kokanica (GenBank X06996 from Melekhovets et al (1988)) with conserved regulatory elements. The second (Ψ5S2) and third (Ψ5S3) genes had a 40 bp deletion at the 5′end and contained several mutations in regulatory boxes.…”

Section: Resultsmentioning

confidence: 97%

Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation

Garcia

Kovařı́k

2013

Heredity

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In higher eukaryotes, the 5S rRNA genes occur in tandem units and are arranged either separately (S-type arrangement) or linked to other repeated genes, in most cases to rDNA locus encoding 18S–5.8S–26S genes (L-type arrangement). Here we used Southern blot hybridisation, PCR and sequencing approaches to analyse genomic organisation of rRNA genes in all large gymnosperm groups, including Coniferales, Ginkgoales, Gnetales and Cycadales. The data are provided for 27 species (21 genera). The 5S units linked to the 35S rDNA units occur in some but not all Gnetales, Coniferales and in Ginkgo (∼30% of the species analysed), while the remaining exhibit separate organisation. The linked 5S rRNA genes may occur as single-copy insertions or as short tandems embedded in the 26S–18S rDNA intergenic spacer (IGS). The 5S transcript may be encoded by the same (Ginkgo, Ephedra) or opposite (Podocarpus) DNA strand as the 18S–5.8S–26S genes. In addition, pseudogenised 5S copies were also found in some IGS types. Both L- and S-type units have been largely homogenised across the genomes. Phylogenetic relationships based on the comparison of 5S coding sequences suggest that the 5S genes independently inserted IGS at least three times in the course of gymnosperm evolution. Frequent transpositions and rearrangements of basic units indicate relatively relaxed selection pressures imposed on genomic organisation of 5S genes in plants.

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Section: Resultsmentioning

confidence: 97%

Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation

Garcia

Kovařı́k

2013

Heredity

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“…1E). The three putative 5S rRNA genes have diverged from each other, although their overall similarity to the sequenced cytosolic 5S rRNA from E. kokanica (Melekhovets et al , 1988) (GenBank accession number X06996) remained high (Supplementary Data Fig. S1B).…”

Section: Resultsmentioning

confidence: 99%

Remarkable variation of ribosomal DNA organization and copy number in gnetophytes, a distinct lineage of gymnosperms

et al. 2018

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Gnetophytes are distinct from other gymnosperms and angiosperms as they display surprisingly large variability in rDNA organization and rDNA copy and locus numbers between genera, with no relationship between copy numbers and genome sizes apparent. Concerted evolution of 5S rDNA units seems to have led to the amplification of 5S pseudogenes in both G. montanum and E. altissima. Evolutionary patterns of rDNA show both gymnosperm and angiosperm features underlining the diversity of the group.

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Evolution of green plants and their relationship with other photosynthetic eukaryotes as deduced from 5S ribosomal RNA sequences

et al. 1990

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Nucleotide sequences of cytosolic 5S ribosomal RNAs from two gymnosperms,Gnetum gnemonandEphedra kokanica

Cited by 7 publications

References 4 publications

Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation

Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation

Remarkable variation of ribosomal DNA organization and copy number in gnetophytes, a distinct lineage of gymnosperms

Evolution of green plants and their relationship with other photosynthetic eukaryotes as deduced from 5S ribosomal RNA sequences

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