Nucleotide variability of protamine genes influencing bull sperm motility variables

Yathish, H.M.; Chaudhary, Rajni; Mishra, Chinmoy; Sivakumar, Aishwarya; Kumar, Amit; Chauhan, Anuj; Ghosh, Subrata; Mitra, Abhijit

doi:10.1016/j.anireprosci.2018.04.060

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…Reduced CRISP2 protein levels were found in azoospermia ( Du et al, 2006 ) and asthenozoospermia ( Jing et al, 2011 ; Heidary et al, 2019 ). Transition nuclear proteins ( TNP1 and TNP2 ) have a role in motility, chromatin integrity, and nucleo-histone to nucleo-protamine transition ( Meistrich et al, 2003 ; Oliva, 2006 ; Selvaraju et al, 2018 ; Yathish et al, 2018 ). The lack of TNP1 and TNP2 leads to defects in sperm head, reduced sperm motility, and infertility ( Adham et al, 2001 ; Shirley et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

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Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction

Prakash

Kumaresan

King

et al. 2021

Front. Cell Dev. Biol.

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Crossbred bulls produced by crossing Bos taurus and Bos indicus suffer with high incidence of infertility/subfertility problems; however, the etiology remains poorly understood. The uncertain predictability and the inability of semen evaluation techniques to maintain constant correlation with fertility demand for alternate methods for bull fertility prediction. Therefore, in this study, the global differential gene expression between high- and low-fertile crossbred bull sperm was assessed using a high-throughput RNA sequencing technique with the aim to identify transcripts associated with crossbred bull fertility. Crossbred bull sperm contained transcripts for 13,563 genes, in which 2,093 were unique to high-fertile and 5,454 were unique to low-fertile bulls. After normalization of data, a total of 776 transcripts were detected, in which 84 and 168 transcripts were unique to high-fertile and low-fertile bulls, respectively. A total of 176 transcripts were upregulated (fold change > 1) and 209 were downregulated (<1) in low-fertile bulls. Gene ontology analysis identified that the sperm transcripts involved in the oxidative phosphorylation pathway and biological process such as multicellular organism development, spermatogenesis, and in utero embryonic development were downregulated in low-fertile crossbred bull sperm. Sperm transcripts upregulated and unique to low-fertile bulls were majorly involved in translation (biological process) and ribosomal pathway. With the use of RT-qPCR, selected sperm transcripts (n = 12) were validated in crossbred bulls (n = 12) with different fertility ratings and found that the transcriptional abundance of ZNF706, CRISP2, TNP2, and TNP1 genes was significantly (p < 0.05) lower in low-fertile bulls than high-fertile bulls and was positively (p < 0.05) correlated with conception rate. It is inferred that impaired oxidative phosphorylation could be the predominant reason for low fertility in crossbred bulls and that transcriptional abundance of ZNF706, CRISP2, TNP2, and TNP1 genes could serve as potential biomarkers for fertility in crossbred bulls.

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Section: Discussionmentioning

confidence: 99%

“…−2.74 Acrosome reaction, penetration of zona pellucida, and spermatogenesis Selvaraju et al, 2018;Yathish et al, 2018 8.…”

Section: Tnp2mentioning

confidence: 99%

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Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction

Prakash

Kumaresan

King

et al. 2021

Front. Cell Dev. Biol.

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“…Fries only, and also 19 SNPs in different regions of TNP2 gene common to all studied breeds (Ramendra et al, 2017). In other candidate genes of sperm motility such as CatSper1 and CatSper2 (Modi, 2011;Sivakumar, 2014), PRM1 and PRM2 genes (Yathish, 2015;Yathish et al, 2018) nucleotide variations have been identified and found their significant influence on sperm motility parameters.…”

Section: Association Of Nucleotide Variabilities In Tnp Genes With mentioning

confidence: 99%

“…Therefore, to obtain a reliable profiling of TNP mRNA, more number of bulls with contrasting semen quality is essential. Profiling of other candidate genes of sperm motility was performed in Vrindavani bulls (Sivakumar, 2014;Yathish, 2015;Yathish et al, 2017Yathish et al, , 2018Yathish, Kumar, Sivakumar, Dubey, & Ghosh, 2014).…”

Section: Expression Profiling Of Tnps Genesmentioning

confidence: 99%

Identification of sperm motility markers in bovine transition protein genes

Maheshwarappa

Kumar

Chaudhary

et al. 2018

Reprod Domestic Animals

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Contents Transition proteins (TNPs) are essential in chromatin condensation during spermiogenesis, and hence, they are the candidate genes for identifying sperm motility markers. Coding and in silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle, and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR‐SSCP analysis revealed two band patterns in fragment III of TNP1 and fragment II of TNP2 gene. Sequence analysis revealed a deletion of “G” nucleotide in 3′UTR region of TNP1 and C>T SNP in intronic region of TNP2 gene. Least square analysis of variance did not reveal any significant influence of nucleotide deletion on any sperm motility parameters in both crossbred and purebred cattle. However, C>T SNP had a significant effect on initial progressive motility (p < 0.05) in purebred cattle and post‐thaw motility in overall cattle population. RT‐qPCR analysis did not reveal any significant variation in TNP1 and TNP2 gene expression among poorly motile and good quality spermatozoa of Vrindavani bulls.

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CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study

Ahmed,

Liu,

Huang

et al. 2024

J Assist Reprod Genet

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Nucleotide variability of protamine genes influencing bull sperm motility variables

Cited by 10 publications

References 46 publications

Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction

Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction

Identification of sperm motility markers in bovine transition protein genes

CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study

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