Nucleus-specific expression in the multinuclear mushroom-forming fungus
            <i>Agaricus bisporus</i>
            reveals different nuclear regulatory programs

Gehrmann, Thies; Pelkmans, Jordi F.; Ohm, Robin A.; Vos, Aurin M.; Sonnenberg, A.S.M.; Baars, J.J.P.; Wösten, Han A. B.; Reinders, Marcel J. T.; Abeel, Thomas

doi:10.1073/pnas.1721381115

Cited by 55 publications

(75 citation statements)

References 38 publications

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“…Compared with traditional metabolic engineering strategies, genome engineering allows rapid tracking and discovery of novel determinants (Xiao and Zhao, 2014;Si et al, 2017), editing of key determinant with single-nucleotide precision (Garst et al, 2017;Bao et al, 2018), or simultaneous manipulating multiple pathways (Barbieri et al, 2017;Liang et al, 2017). Apart from the unicellular model organisms (e.g., Saccharomyces cerevisiae), many filamentous fungi, particularly the mushroom-forming fungi, contain two different nuclei with different genetic contents (Gehrmann et al, 2018). In addition to the heterogeneity, many important medicinal mushrooms also exhibit low efficiency on gene transformation and homologous recombination (HR), which pose a great challenge to establish gene editing tools for genome engineering (Wang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Wang

Zhong

Xiao

2020

Front. Bioeng. Biotechnol.

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Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.

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Section: Discussionmentioning

confidence: 99%

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Wang

Zhong

Xiao

2020

Front. Bioeng. Biotechnol.

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“…Although mature fruiting bodies of different species differ greatly in morphology, the genes regulating their development are highly conserved [11][12][13][14][15][16][17][18]. Previous studies have reported some genes involved in the fruiting body development in some species in Basidiomycete [11,[19][20][21][22][23]. Research on Pleurotus ostreatus fruiting body development, especially on stipe elongation, is very limited.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation

Zhu

Li³

et al. 2019

IJMS

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Pleurotus ostreatus is a widely cultivated edible fungus around the world. At present, studies on the developmental process of the fruiting body are limited. In our study, we compared the differentially expressed proteins (DEPs) in the stipe and cap of the fruiting body by high-throughput proteomics. GO and pathway analysis revealed the great differences in the metabolic levels, including sucrose and starch metabolism, and sphingolipid signaling and metabolism, and the differences of 16 important DEPs were validated further by qPCR analysis in expression level. In order to control the cap and stipe development, several chemical inducers were applied to the primordium of the fruiting body according to the pathway enrichment results. We found that CaCl2 can affect the primordium differentiation through inhibiting the stipe development. EGTA (ethyleneglycol bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid) treatment confirmed the inhibitory role of Ca2+ in the stipe development. Our study not only shows great metabolic differences during the cap and stipe development but also reveals the underlying mechanism directing the primordium differentiation in the early development of the fruiting body for the first time. Most importantly, we provide a reliable application strategy for the cultivation and improvement of the Pleurotus ostreatus, which can be an example and reference for a more edible fungus.

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“…Fungi form diverse relationships with viruses, algae, plants, animals, insects, and bacteria, and are critical to human health [15][16][17][18][19][20][21]. Filamentous fungi, in particular, are distinguished by their multinucleate cells, known as hyphae, chitinous-composite cell walls, vast repertoire of degradative enzymes and secondary metabolites, and interconnected networks of cells, known as mycelium, that form through a combination of apical growth, hyphal branching, and regulated self-fusion [22][23][24][25][26][27][28][29][30][31]. Growing mycelium penetrate and degrade the substrate upon which the organism lives and uptake and shuttle nutrients throughout complex hyphal networks [23].…”

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confidence: 99%

Enabling community-based metrology for wood-degrading fungi

Perez

Luccioni

Kamakaka

et al. 2020

Fungal Biol Biotechnol

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Background: Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi could enable more direct means of manufacture. However, basic methods for cultivating wood-degrading fungi are often ad hoc and not readily reproducible. Here, we developed standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products in simple laboratory settings. Results:We show that a widely-available and globally-regularized consumer product (Pringles ™ ) can support the growth of wood-degrading fungi, and that growth on Pringles ™ -broth can be correlated with growth on media made from a fully-traceable and compositionally characterized substrate (National Institute of Standards and Technology Reference Material 8492 Eastern Cottonwood Whole Biomass Feedstock). We also establish a Relative Extension Unit (REU) framework that is designed to reduce variation in quantification of radial growth measurements. So enabled, we demonstrate that five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay, and that our REU-based approach reduced variation in reported measurements by up to ~ 75%. Conclusions:Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. Our community-based measurement methods incentivize practitioners to coordinate the reuse of standard materials, methods, strains, and to share information supporting work with wood-degrading fungi.

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Nucleus-specific expression in the multinuclear mushroom-forming fungus Agaricus bisporus reveals different nuclear regulatory programs

Cited by 55 publications

References 38 publications

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation

Enabling community-based metrology for wood-degrading fungi

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