Nuevos tratamientos de la enfermedad arterial periférica oclusiva no revascularizable: angiogenia terapéutica

Lara-Hernández, R.; Lozano-Vilardell, P.; Cordobés-Gual, J.

doi:10.1157/13128727

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Although therapies involving cytokine-transduced EPCs (e.g., SDF-1 and VEGF) can have profound and multifaceted effects on neovascularization [16], [17], the results in phase I/IIa of clinical trials show certain adverse effects. For instance, in a VEGF trial, 60% of the patients developed moderate or severe edema [18], [19]. The effects of therapy involving cytokine-transduced EPCs for wound healing have not yet been proven in a controlled clinical trial.…”

Section: Introductionmentioning

confidence: 99%

Kallikrein Gene-Modified EPCs Induce Angiogenesis in Rats with Ischemic Hindlimb and Correlate with Integrin αvβ3 Expression

Wang

et al. 2013

PLoS ONE

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BackgroundHuman tissue kallikrein (hTK) plays an essential role in the physiological and pathological mechanisms of blood vessels. This study aimed to determine whether angiogenesis induced by endothelial progenitor cells (EPCs) transduced with the adenovirus-mediated hTK gene could improve blood flow in rat hindlimb ischemia in vivo and to establish a promising mechanism in vitro.MethodsEPCs transduced with adenovirus encoding hTK-162 (i.e., Ad/hTK-transduced EPCs or Ad/GFP-transduced EPCs) were administered to Wister rats with hindlimb ischemia through therapeutic neovascularization. Muscular capillary density (MCD), blood flow (BF), and the number of myofibers were measured at days 7, 14, and 21 after treatment. Expressions of integrin αvβ3 and endothelial nitric oxide synthase (eNOS) were detected on the surface of EPCs.ResultsMCD, BF, and the number of myofibers in rats with Ad/hTK-transduced EPCs remarkably increased at day 21 after treatment compared with rats with Ad/GFP-transduced EPCs or the control group (P<0.01). Expressions of integrin αvβ3 and eNOS protein on the surface of EPCs also increased in rats with Ad/hTK-transduced EPCs. The levels of integrin αvβ3 expression were reduced by PI3K and eNOS blockade, and the inhibitor of integrin αvβ3 abrogated the migration and adhesion of hTK-transduced EPCs (P<0.05).Conclusion hTK gene delivery in vivo improves the natural angiogenic response to ischemia. The ability of hTK gene-transduced EPCs can be enhanced in vitro, in which integrin αvβ3 plays a role in the process.

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Section: Introductionmentioning

confidence: 99%

Kallikrein Gene-Modified EPCs Induce Angiogenesis in Rats with Ischemic Hindlimb and Correlate with Integrin αvβ3 Expression

Wang

et al. 2013

PLoS ONE

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“…En estos casos se aplican alternativas cuyo objetivo es paliar los síntomas y los efectos de esta enfermedad. Entre estas medidas se encuentran la simpatectomía lumbar, la administración de prostaglandinas por vía parenteral, la angiogénesis terapéutica y la neuroestimulación eléctrica espinal (20,(27)(28)(29).…”

Section: Contraindicación Relativaunclassified

Efectos termocutáneos de la anestesia epidural lumbar en las extremidades inferiores de sujetos sanos y con arteriopatía crónica

Rodríguez¹

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Efectividad de la simpatectomía lumbar en el tratamiento de la arteriopatía periférica oclusiva de miembros inferiores: revisión sistemática

Ruiz-Aragón

Calderón

2010

Medicina Clínica

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Nuevos tratamientos de la enfermedad arterial periférica oclusiva no revascularizable: angiogenia terapéutica

Cited by 7 publications

References 41 publications

Kallikrein Gene-Modified EPCs Induce Angiogenesis in Rats with Ischemic Hindlimb and Correlate with Integrin αvβ3 Expression

Kallikrein Gene-Modified EPCs Induce Angiogenesis in Rats with Ischemic Hindlimb and Correlate with Integrin αvβ3 Expression

Efectos termocutáneos de la anestesia epidural lumbar en las extremidades inferiores de sujetos sanos y con arteriopatía crónica

Efectividad de la simpatectomía lumbar en el tratamiento de la arteriopatía periférica oclusiva de miembros inferiores: revisión sistemática

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