Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes

Cleveland, Don W.; Lopata, Margaret A.; MacDonald, Raymond J.; Cowan, Nicholas J.; Rutter, William J.; Kirschner, Marc W.

doi:10.1016/0092-8674(80)90238-x

Cited by 1,832 publications

(619 citation statements)

References 34 publications

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“…Hybond-N membranes, multiprime DNA-labelling system, [c~-a2P]dCTP (3000 Ci/mmol), [~-32P]UTP (800 Ci/mmol) and Hyperfilm MP were from Amersham. The probes used were the full-length chicken ~-actin eDNA provided by Dr. D.W. Cleveland [6], an insert of rat glyceraldehyde-3-phosphate dehydrogenase (GraP-DH) (EC 1.2.1.12) provided by Dr. J.M. Blanchard [7] and an insert of 18S rRNA provided by Dr. L. Hendriks [8], both used as internal control.…”

Section: Methodsmentioning

confidence: 99%

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Husson¹,

Quillard²,

Fairand³

et al. 1996

FEBS Letters

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The mechanism of action of hydration state was studied on ~-actin gene expression in isolated hepatoeytes. Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the ~actin mRNA level through a corresponding modulation of the rate of the gene transcription. Glutamine, which is known to induce cell swelling, also increased the ~-actin mRNA level in a dose-dependent manner and induced a stimulation of the ~-actin gene transcription. Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.

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Section: Methodsmentioning

confidence: 99%

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Husson¹,

Quillard²,

Fairand³

et al. 1996

FEBS Letters

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“…RNA was denatured and electrophoresed through 1.2°70 agarose gels using the glyoxal procedure [18] and transferred to Biodyne (Pall Corp., Glen Cove, NY) membranes. Hybridizations with either the hamster #2-adrenergic receptor [8] or chicken ~-actin [19] cDNA probes were conducted as previously described [20]. These probes were labeled by nick translation with [a-32p]dCTP (3-8 x l0 s dpm/#g DNA).…”

Section: Methodsmentioning

confidence: 99%

Regulation of the β₂‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone

et al. 1988

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To investigate the regulation of fl2AR expression in rat ventral prostate the effects of castration and testosterone replacement on the fl2AR were studied by ligand binding and Northern blot analysis. Orchidectomy depressed fl2AR number by 50% within 4 days and testosterone administration to 4-day castrates produced a rapid and complete recovery offl2AR number within 24 h. In contrast to receptor number, fl2AR mRNA levels did not change relative to total RNA following castration. However, during the testosterone replacement period fl2AR mRNA levels rose transiently, reaching a maximum (3.5-fold) between 8 and 12 h, and this increase in mRNA preceded the recovery in B2AR number in the membrane. Regulation of fl2AR gene expression by testosterone in the ventral prostate is thus complex and probably involves both transcriptional and post-transcriptional components.fl2-Adrenergic receptor; Androgen; Ventral prostate; mRNA

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“…The &subunit specific probe is a genomic Hind111 fragment of about 1500 bp and contains sequences corresponding to the putative protein coding region P7 plus additional 3'-and 5'-flanking sequences (unpublished). The chicken @actin cDNA probe used was a gift from B. Paterson and represents the -2000 bp PstI fragment described by Cleveland et al [13].…”

Section: Hybridization Probesmentioning

confidence: 99%

Developmental regulation of five subunit specific mRNAs encoding acetylcholine receptor subtypes in rat muscle

et al. 1989

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The muscular content of the mRNAs encoding the five subunits of the nicotinic acetylcholine receptor was measured during postnatal development in the rat. Subunit specific mRNAs show differchtial regulation. The levels of the a-, yand &subunit specific mRNAs decrease steadily after birth, while the & and a-subunit mRNAs increase transiently and then decrease. The adult pattern of subunit specific mRNA levels is reached at 46 weeks postnatally. The content of y-and e-subunit mRNA changes in a reciprocal fashion during the first 2 postnatal weeks, supporting the view that differential regulation of y-and s-subunit mRNA during development is one mechanism mediating the appearance of the adult, s-subunit containing, subtype of end-plate channel. Denervation of neonatal muscle increases the levels of all subunitspecific mRNAs during further development. It prevents the postnatal decrease in y-subunit mRNA and enhances the initial increase in a-subunit mRNA. This makes it appear that the e-subunit gene is less sensitive to regulation by the nerve in the postnatal period than the y-subunit gene.

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Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes

Cited by 1,832 publications

References 34 publications

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Regulation of the β₂‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone

Developmental regulation of five subunit specific mRNAs encoding acetylcholine receptor subtypes in rat muscle

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Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes

Cited by 1,832 publications

References 34 publications

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

Regulation of the β2‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone

Developmental regulation of five subunit specific mRNAs encoding acetylcholine receptor subtypes in rat muscle

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Regulation of the β₂‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone