Number and Proliferative Capacity of Osteogenic Stem Cells Are Maintained During Aging and in Patients with Osteoporosis

Stenderup, Karin; Justesen, Jeannette; Eriksen, Erik Fink; Rattan, Suresh I. S.; Kassem, Moustapha

doi:10.1359/jbmr.2001.16.6.1120

Cited by 224 publications

(131 citation statements)

References 47 publications

Supporting

Mentioning

119

Contrasting

Unclassified

Order By: Relevance

“…Recently, Stolzing et al (2008) confirmed those already demonstrated in previous studies (D 'Ippolito et al 1999, Mendes et al 2002, Chen et al 2005, Zhang et al 2005, Zheng et al 2007, Kretlow et al 2008, observing a significant age-related change in membrane markers expression levels, an alkaline phosphatase activity, chondrogenic and myogenic differentiation potency decline with donor age in contrast with adipogenic differentiation. However, these data are in contrast with those reported by other research groups (Bergman et al 1996, Stenderup et al 2001, Bellows et al 2003, which showed no age-related differences in differentiation potency. Different results obtained are with regard to the choice of age groups, group size, gender, inclusion of pathological states, isolation and cultivation conditions (Sethe et al 2006).…”

Section: Introductioncontrasting

confidence: 99%

Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Iacono

Brunori²,

Pirrone³

et al. 2012

Reproduction

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify and characterize stem cells from equine amniotic fluid (AF), CB and Wharton's Jelly (WJ). Samples were obtained from 13 mares at labour. AF and CB cells were isolated by centrifugation, while WJ was prepared by incubating with an enzymatic solution for 2 h. All cell lines were cultured in DMEM/TCM199 plus fetal bovine serum. Fibroblast-like cells were observed in 7/10 (70%) AF, 6/8 (75%) CB and 8/12 (66.7%) WJ samples. Statistically significant differences were found between cell-doubling times (DTs): cells isolated from WJ expanded more rapidly (2.0G0.6 days) than those isolated from CB (2.6G1.3 days) and AF (2.3G1.0 days) (P!0.05). Positive von Kossa and Alizarin Red S staining confirmed osteogenesis. Alcian Blue staining of matrix glycosaminoglycans illustrated chondrogenesis and positive Oil Red O lipid droplets staining suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105; and negative for CD34, CD14 and CD45. These findings suggest that equine MSCs from AF, UCB and WJ appeared to be a readily obtainable and highly proliferative cell lines from a uninvasive source that may represent a good model system for stem cell biology and cellular therapy applications in horses. However, to assess their use as an allogenic cell source, further studies are needed for evaluating the expression of markers related to cell immunogenicity.

show abstract

Section: Introductioncontrasting

confidence: 99%

Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Iacono

Brunori²,

Pirrone³

et al. 2012

Reproduction

View full text Add to dashboard Cite

show abstract

“…Alkaline phosphatase, which should decline only with differentiation, was found to decrease progressively with the number of passages, while nestin expression was variable in undifferentiated stem cells [2,3]. Furthermore, studies on osteo-genic stem cells [36] and muscle precursor cells [25] indicated that Stro1 is present in rats of all ages. Quantification of the stem cell surface marker Stro1 confirmed that for both MSC and ASC the percentage of Stro1 positive cells was similar for neonates and young adults, but with a decrease in Stro1 positive cells from old animals.…”

Section: Discussionmentioning

confidence: 98%

Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Mantovani

Raimondo

Haneef

et al. 2012

Experimental Cell Research

View full text Add to dashboard Cite

“…There are several reports describing the influence of aging on the properties of bone marrow-derived MSCs, and this topic remains controversial. Some studies have shown that aging does not affect colony-forming efficiency (33)(34)(35), adipogenesis (34), and calcification (33,34,36). In contrast, others have reported that aging affects the proliferative capacity at passage 1 (36) as well as the chondrogenic (36), osteogenic (35,37), and adipogenic (36) differentiation ability of bone marrow-derived MSCs.…”

Section: Discussionmentioning

confidence: 99%

Higher chondrogenic potential of fibrous synovium– and adipose synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans

Mochizuki

Muneta

Sakaguchi

et al. 2006

Arthritis & Rheumatism

278

259

View full text Add to dashboard Cite

Objective. Mesenchymal stem cells from synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous synovium and adipose synovium (components of the synovium with subsynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose synovium-derived cells, fibrous synovium-derived cells, and subcutaneous fat-derived cells to determine their similarities.Methods. Human fibrous synovium, adipose synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared.Results. Fibrous synovium-and adipose synovium-derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat-derived cells. Cells derived from fibrous and adipose synovium had higher proliferative potential and colonyforming efficiency compared with subcutaneous fatderived cells, both in mixed-population and in singlecell-derived cultures. In chondrogenic assays, pellets from fibrous synovium-and adipose synovium-derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous synovium-and adipose synovium-derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors.Conclusion. Cells derived from the fibrous synovium and from the adipose synovium demonstrate comparable chondrogenic potential. Adipose synoviumderived cells are more similar to fibrous synoviumderived cells than to subcutaneous fat-derived cells.

show abstract

Number and Proliferative Capacity of Osteogenic Stem Cells Are Maintained During Aging and in Patients with Osteoporosis

Cited by 224 publications

References 47 publications

Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Higher chondrogenic potential of fibrous synovium– and adipose synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans

Contact Info

Product

Resources

About