Number and Sites of rDNA Loci of Guizotia Abyssinica (L. f.) Cass. as Determined by Fluorescence in Situ Hybridization

Dagne, Kifle; Cheng, Bifang; Heneen, Waheeb K.

doi:10.1111/j.1601-5223.2000.00063.x

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…2). A similar regularity has been previously found in other small chromosome plant species (Xu and Earle 1996;Dagne et al 2000). Concordant changes in C-band size and the number of rRNA genes localized to this region allowed the authors to suggest that a part of the constitutive heterochromatin was associated with NORs, the ribosomal gene clusters dispersed within heterochromatic regions (Mukai et al 1991;Zoldos et al 1999).…”

Section: Discussionsupporting

confidence: 55%

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

et al. 2008

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Karyotypes of species sects. Linum and Adenolinum have been studied using C/DAPI-banding, Ag-NOR staining, FISH with 5S and 26S rDNA and RAPD analysis. C/DAPI-banding patterns enabled identification of all homologous chromosome pairs in the studied karyotypes. The revealed high similarity between species L. grandiflorum (2n = 16) and L. decumbens by chromosome and molecular markers proved their close genome relationship and identified the chromosome number in L. decumbens as 2n = 16. The similarity found for C/DAPI-banding patterns between species with the same chromosome numbers corresponds with the results obtained by RAPD-analysis, showing clusterization of 16-, 18- and 30-chromosome species into three separate groups. 5S rDNA and 26S rDNA were co-localized in NOR-chromosome 1 in the genomes of all species investigated. In 30-chromosome species, there were three separate 5S rDNA sites in chromosomes 3, 8 and 13. In 16-chromosome species, a separate 5S rDNA site was also located in chromosome 3, whereas in 18-chromosome species it was found in the long arm of NOR-chromosome 1. Thus, the difference in localization of rDNA sites in species with 2n = 16, 2n = 30 and 2n = 18 confirms taxonomists opinion, who attributed these species to different sects. Linum and Adenolinum, respectively. The obtained results suggest that species with 2n = 16, 2n = 18 and 2n = 30 originated from a 16-chromosome ancestor.

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Section: Discussionsupporting

confidence: 55%

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

et al. 2008

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“…An in‐depth treatment of its taxonomy and distribution was done by Baagoe (1974). Cytological studies were done by several workers (Hiremath and Murthy 1986; Dagne and Heneen 1992; Hiremath and Murthy 1992; Dagne et al 2000; Dagne 2001). However, to date there is no report of using ISSR markers to study the genetic diversity of niger.…”

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confidence: 99%

Analysis of genetic diversity of Guizotia abyssinica from Ethiopia using inter simple sequence repeat markers

2007

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Within and among population genetic diversity of 37 Guizotia abyssinica populations from Ethiopia were analyzed using inter simple sequence repeats (ISSRs). Five primers amplified a total of 118 genomic DNA fragments across a total of 370 individuals of which 106 were polymorphic (89.83%). The average number of polymorphic bands per primer was 21.2. More bands were generated by primer UBC 888 (BDB(CA)(7.) The total genetic diversity (Ht) and the coefficient of genetic differentiation (Gst) were 0.4115 and 0.0918 respectively, while the within population genetic diversity (Hs) and the among population genetic diversity(Dst) were 0.3738 and 0.03776 respectively suggesting more variability within the populations than among them. The standard genetic distances between the G. abyssinica populations of the eight regions ranged from 0.0281 (between Wollo and Gojam) to 0.1148 (between Jimma and Hararghe). Generally, the standard genetic distances are smaller between populations of neighboring regions and highest between those of Jimma and the other regions, ranging from 0.0696 (between Jimma and Shewa) to 0.1148 (between Jimma and Hararghe). The ISSR based UPGMA clustering using the standardized genetic distances matrix also placed populations from neighboring regions closer than those from farther apart areas, while the UPGMA clustering by regions based on the standard genetic distances produced three clusters following the proximity and the contiguity of the regions. The mean Shannon Weaver diversity indices for the populations of the eight regions ranged from 0.8197 (Jimma) to 0.9176 (Hararghe), with a mean of 0.8841 for the whole material.

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Neglected Oil Crop Biotechnology

Tiwari

Kumar

2013

Biotechnology of Neglected and Underutilized Crops

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Number and Sites of rDNA Loci of Guizotia Abyssinica (L. f.) Cass. as Determined by Fluorescence in Situ Hybridization

Cited by 5 publications

References 8 publications

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

Analysis of genetic diversity of Guizotia abyssinica from Ethiopia using inter simple sequence repeat markers

Neglected Oil Crop Biotechnology

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