Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: Empirical and calculation methods

Epstein, H.; Afergan, Eyal; Moise, Tamar; Richter, Yoram; Rudich, Yinon; Golomb, Gershon

doi:10.1016/j.biomaterials.2005.06.006

Cited by 43 publications

(35 citation statements)

References 28 publications

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“…The liposomes used here (111 nm average diameter) contained approximately 10 3 Mn(II) ions each. A suspension containing 0.06 mM Mn(II) ions corresponds to 30 nM concentration of liposomes (determined by fluorimetric measurement, which was in agreement with a calculation by the method of Epstein et al [45]). This sample was readily detectable in the R 1 image, indicating that the use of FFC-MRI with the tailored liposomal agent does indeed give rise to high sensitivity of detection.…”

Section: Ffc-mri With Contrast Agentssupporting

confidence: 86%

Fast field-cycling magnetic resonance imaging

Lurie

Aime

Baroni

et al. 2010

Comptes Rendus. Physique

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Fast-field cycling magnetic resonance imaging FFC-MRI Earth-field MRI Pre-polarised MRI Free-radical imaging Magnetisation-transfer contrast Mots-clés : IRM en champ cyclé FFC-IRM IRM en champ magnétique terrestre IRM prépolarisée Imagerie par radicaux libres Contraste par transfert de magnetisationMagnetic resonance imaging (MRI) and fast field-cycling (FFC) NMR are both welldeveloped methods. The combination of these techniques, namely fast field-cycling magnetic resonance imaging (FFC-MRI) is much less well-known. Nevertheless, FFC-MRI has a number of significant applications and advantages over conventional techniques, and is being pursued in a number of laboratories. This article reviews the progress in FFC-MRI over the last two decades, particularly in the areas of Earth's field and prepolarised MRI, as well as free radical imaging using field-cycling Overhauser MRI. Different approaches to magnet design for FFC-MRI are also described. The paper then goes on to discuss recent techniques and applications of FFC-MRI, including protein measurement via quadrupolar cross-relaxation, contrast agent studies, localised relaxometry and FFC-MRI with magnetisation-transfer contrast. r é s u m é L'imagerie par Résonance Magnétique (IRM, ou MRI en anglais) et la RMN avec cyclage de champ rapide (« fast field cycling », FFC) sont toutes deux des méthodes bien développées. La combinaison de ces techniques, c'est-à-dire l'imagerie par résonance magnétique avec cyclage de champ rapide (FFC-MRI en anglais) est beaucoup moins bien connue. Cependant, la FFC-MRI a un nombre d'applications et d'avantages significatifs par rapport aux techniques conventionnelles, et son étude est poursuivie dans un certain nombre de laboratoires. Cet article passe en revue les progrès de la FFC-MRI au cours des deux dernières décennies, en particulier dans les domaines de l'imagerie en champ terrestre avec pré-polarisation, de même que l'imagerie des radicaux libres en utilisant la FFC-MRI avec effet Overhauser. Diverses approches à la conception des aimants pour FFC-MRI sont également décrites. L'article continue en discutant des techniques et applications récentes de la FFC-MRI, telles que la mesure des protéines par relaxation croisée quadrupolaire, les études d'agents de contraste, la relaxométrie localisée et la FFC-MRI avec contraste par transfert d'aimantation.

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Section: Ffc-mri With Contrast Agentssupporting

confidence: 86%

Fast field-cycling magnetic resonance imaging

Lurie

Aime

Baroni

et al. 2010

Comptes Rendus. Physique

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“…3,4 Although the particle size can be easily monitored by many techniques, such as electron microscopy or dynamic laser scattering (DLS), the number-concentration measurement of these nanoparticles in a given liquid still remains a challenge. 10,11 Some calculation methods for number-concentration determination that have been employed previously are limited because they rely on several assumptions and are not applicable for all preparations. 11,12 A reasonable result can only be obtained for monodisperse spherical particles with precise uniform size, such as NIST standard polystyrene (PS) beads, which meet the limiting assumptions very well.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 Some calculation methods for number-concentration determination that have been employed previously are limited because they rely on several assumptions and are not applicable for all preparations. 11,12 A reasonable result can only be obtained for monodisperse spherical particles with precise uniform size, such as NIST standard polystyrene (PS) beads, which meet the limiting assumptions very well. So far, experimental methods for nanoparticle counting are indirect, typically detecting a pulsed signal from a single nanoparticle in liquid and counting them individually, which is usually based on two detection methods.…”

Section: Introductionmentioning

confidence: 99%

Measuring number‐concentrations of nanoparticles and viruses in liquids on‐line

Du¹,

Kendall²,

Morris³

et al. 2010

J of Chemical Tech & Biotech

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BACKGROUND: The surge of studies on artificial and natural nanoparticles has revealed a new world for engineering and life sciences, but in most instances, the small scale has made their number-concentration determination in liquids a challenging problem. Former success has mostly been limited to special particles measured by indirect techniques. A new approach is required for this determination to facilitate the production and application of nanoparticles in different systems.

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“…The number of liposomes was determined by N VOL method. 238 As illustrated in Figure 4.2, both micellar conjugate and liposomes were incubated together at 48°C for 6 min and stored overnight at 4°C. The liposome formulation was then centrifuged (1,500xg, 15 min) in mini bio-spin chromatography columns to remove any unincorporated conjugate.…”

Section: Insertion Of Dspe-peg 2000 -Maleimide-crgd Conjugate Into LImentioning

confidence: 99%

“…For liposome systems, the entrapped aqueous volume is estimated by N VOL method. 238 The aggregation process in colloidal systems is a second-order process. Mean diameter (D t ) of liposomes at time, t is determined from Equation 1.4:…”

mentioning

confidence: 99%

Targeted Delivery of Surface Modified Nanoparticles: Modulation of Inflammation for Acute Lung Injury

Desu¹

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Number-concentration of nanoparticles in liposomal and polymeric multiparticulate preparations: Empirical and calculation methods

Cited by 43 publications

References 28 publications

Fast field-cycling magnetic resonance imaging

Fast field-cycling magnetic resonance imaging

Measuring number‐concentrations of nanoparticles and viruses in liquids on‐line

Targeted Delivery of Surface Modified Nanoparticles: Modulation of Inflammation for Acute Lung Injury

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