Numerical aberrations of chromosomes 8, 9, 11, and 17 in squamous cell carcinoma of the pharynx and larynx: a fluorescence in situ hybridization and DNA flow cytometric analysis of 50 cases

Hardisson, David; Álvarez-Marcos, César; Salas-Bustamante, Ana; Alonso-Guervós, Marta; Sastre, Noelia; Sampedro, Andrés Gené

doi:10.1016/j.oraloncology.2003.09.008

Cited by 16 publications

(14 citation statements)

References 22 publications

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“…29,30 Chromosome 8, chromosome 17, and MYC were chosen for the current analysis based on the literature and our previous studies. [20][21][22][23]25,31 Chromosome 8 is the site of several genes that have crucial functions in the tumorigenesis of several solid and hematopoietic malignancies.…”

Section: Discussionmentioning

confidence: 99%

“…Chromosome 8 was analyzed successfully to detect ACs in premalignant and malignant lesions in the oral mucosa. 20,21 Gains of chromosomes 8 and 17 reportedly are altered in LSCC, 18,24,25 and 8q was the most common arm gain. 26,27 These chromosomal probes can disclose numerical as well as structural aberrations; for example, 3 signals of 8q can be detected even if the centromeric 8 probe yields 2 signals (ie, gains of the MYC gene, which is located on 8q24).…”

Section: Combined Morphology and Fish Analysismentioning

confidence: 98%

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Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

Shani

Primov-Fever

Wolf

et al. 2011

Cancer Cytopathology

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BACKGROUND:Most cases of laryngeal cancer are preceded by precursor lesions which, if left untreated, can progress toward an invasive cancer. The objective of this study was to investigate the presence of chromosomal numerical aberrations in cells that were collected by noninvasive brush sampling from laryngeal lesions.METHODS:Laryngeal brush samples from 52 patients were analyzed simultaneously for morphology and fluorescence in situ hybridization (FISH) using centromeric probes for chromosome 17, chromosome 8, and a locus‐specific instability (LSI) v‐myc avian myelocytomatosis viral oncogene homolog (myc) proto‐oncogene protein (C‐MYC) probe for the MYC gene. The patients were divided according to histopathologic diagnosis. Group 1 included patients with squamous cell carcinoma, carcinoma in situ, and severe dysplasia; Group 2 included patients with moderate dysplasia, mild dysplasia, and hyperplasia; and Group 3 included patients with benign nondysplastic lesions.RESULTS:The proportion of cells with MYC and chromosome 8 gains demonstrated significant trends toward being the highest in Group 1 and the lowest in Group 3 (P = .001 and P = .003, respectively). No significant trend was observed for chromosome 17. Mann‐Whitney Bonferroni‐corrected analyses revealed that the most significant contribution was the difference between Groups 1 and 3 (P = .0195 for MYC gains and P = .036 for chromosome 8 gains). When using a cutoff point of 4% aneuploid cells (ACs), both MYC and chromosome 8 differed significantly between groups (P = .030 and P = .037, respectively).CONCLUSIONS:The current results suggested that FISH analysis of brush samples obtained noninvasively from suspicious laryngeal lesions can augment the clinical examination in predicting the nature of the lesions and can aid clinicians in monitoring and follow‐up of high‐risk patients. Cancer (Cancer Cytopathol) 2011. © 2011 American Cancer Society.

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Section: Discussionmentioning

confidence: 99%

Section: Combined Morphology and Fish Analysismentioning

confidence: 98%

Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

Shani

Primov-Fever

Wolf

et al. 2011

Cancer Cytopathology

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“…Estos resultados están dentro del rango de porcentajes informados en la literatura (18,21,22 (17,18,20,22). Lo anterior ha permitido sugerir que la monosomía del cromosoma 17 es una alteración común en los tumores sólidos y que podría considerarse como un marcador cromosómico recurrente.…”

Section: Discussionunclassified

“…En conclusión, la aneuploidía del cromosoma 17 afecta notablemente la expresión de múltiples genes, estableciéndose una vía molecular relacionada con el origen de diversos tumores, como se ha observado en el de mama, colorrectal, ovario, vejiga, cabeza y cuello (4,8,9,10,20,22,26).…”

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Detección de aneuploidías del cromosoma 17 y deleción del gen TP53 en una amplia variedad de tumores sólidos mediante hibridación in situ fluorescente bicolor

et al. 2010

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Detección de aneuploidías del cromosoma 17 y deleción del genTP53 en una amplia variedad de tumores sólidos mediante hibridación in situ fluorescente bicolor Introducción. El TP53 es un gen supresor de tumores localizado en la región cromosómica 17p13.1; controla el ciclo celular y se encuentra alterado en cerca de 50% de todas las neoplasias. Objetivos. Determinar las aneuploidías del cromosoma 17 y la deleción en el locus 17p13.1 del gen TP53, en diversos tumores sólidos primarios utilizando la técnica FISH-bicolor. Materiales y métodos. Se analizaron 38 muestras de diversos tipos de tumores sólidos primarios. Todas las muestras se disociaron mecánica y enzimáticamente con colagenasa al 0,2%, antes de la obtención de los núcleos interfásicos. La técnica de FISH-bicolor se realizó en núcleos interfásicos, mediante sondas marcadas directamente con fluorocromos para el centrómero del cromosoma 17 (CEP 17; señal verde; VYSIS) y para el locus específico del gen TP53 (LSI 17p13.1; señal naranja; VYSIS). Resultados. Se encontró que 63% (24/38) de las muestras tenían aneuploidías del cromosoma 17. La monosomía fue la aneuploidía más frecuente (75%; 18/24), seguida de la trisomía (17%; 4/24); la nulisomía y la tetrasomía fueron menos frecuentes. El 89,5% (34/38) de los casos presentaron deleción del gen TP53. Sólo cuatro casos fueron normales para el número de copias del cromosoma 17 y del gen TP53. El estudio histopatológico mostró que la mayoría de las muestras eran tumores malignos. Conclusiones. La aneuploidía del cromosoma 17 y la deleción en el locus 17p13.1 del gen TP53 son alteraciones muy frecuentes en los tumores sólidos. La técnica FISH-bicolor permite detectar simultáneamente alteraciones cromosómicas numéricas y estructurales en núcleos interfásicos.Palabras clave: aneuploidía, deleción cromosómica, gen Tp53, hibridación fluorescente in situ, neoplasias, inestabilidad cromosómica, heterogeneidad genética. Detection of chromosome 17 aneuplody and TP53 gene deletion in a broad variety of solid tumors by dual-color fluorescence in situ hybridization (FISH)Introduction. TP53 is a tumor suppressor gene located on chromosome 17p13.1. This gene is essential for the control of cell cycle and has been found altered in about 50% of all tumor types. Objective. The presence of aneuploidy of chromosome 17 and TP53 gene deletion at 17p13.1 locus was determined in primary solid tumors using the dual-color FISH (fluorescence in situ hybridization). Materials and methods. Thirty-eight samples consisted of several types of primary solid tumors. All samples were mechanically and enzymatically disaggregated with 0.2% collagenase prior to obtaining interphase nuclei. The dual-color FISH was performed using direct fluorescent labeling probes for the chromosome 17 centromere (green signal) and for the TP53 gene locus-specific (orange signal).Results. Characteristic aneuploidy on chromosome 17 was found in 63% (24/38) of the samples. Monosomy occurred most frequently (75%, 18/24), followed by trisomy (17%, 4/24); nullisomy and te...

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Correlation of Numerical Aberrations of Chromosomes X and 11 and Poor Prognosis in Squamous Cell Carcinomas of the Head and Neck

Xie¹,

Clausen²,

Boysen³

2006

Arch Otolaryngol Head Neck Surg

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To investigate the prognostic significance of chromosomal aberrations of chromosomes X and 11 in relation to disease-specific survival in head and neck squamous cell carcinoma. Setting: University hospital. Design: A 10-year retrospective clinical study. Information about clinical findings, treatment, and follow-up has been recorded prospectively. Patients: By means of the fluorescence in situ hybridization technique with centromeric probes for chromosomes X and 11, we analyzed 40 randomly selected patients before treatment for T1 to T4 head and neck squamous cell carcinoma. Numerical aberrations were scored and evaluated in frozen sections. Main Outcome Measures: The significance of prognostic parameters was tested by the log-rank and Kaplan-Meier methods for the univariate analysis. The Cox proportional hazards regression model was used for multivariate analysis. Results: Numerical aberrations of chromosome 11 correlated positively with T and N classification (P=.03 and P=.02, respectively) and with clinical stage (P=.02). Patients with higher frequencies of numerical aberrations for both chromosome X (Ͼ48%, mean) and chromosome 11 (Ͼ57%, mean) had shortened disease-specific survival compared with those with lower frequencies of numerical aberrations (P=.008 and PϽ.001, respectively). Of patients who died from disease within 3 years, 7 (50%) had a trisomic value of chromosome 11 of 35% or higher of nuclei (PϽ.001). Moreover, patients with a higher value (Ն8%) of amplification of chromosome 11 (Ͼ4 signals) were associated with having poor prognosis compared with those with a lower value (P=.02). Conclusion: Numerical aberrations of chromosomes X and 11 had prognostic value in head and neck squamous cell carcinoma, and higher frequencies of numerical aberrations correlated with poor prognosis.

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Numerical aberrations of chromosomes 8, 9, 11, and 17 in squamous cell carcinoma of the pharynx and larynx: a fluorescence in situ hybridization and DNA flow cytometric analysis of 50 cases

Cited by 16 publications

References 22 publications

Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

Detección de aneuploidías del cromosoma 17 y deleción del gen TP53 en una amplia variedad de tumores sólidos mediante hibridación in situ fluorescente bicolor

Correlation of Numerical Aberrations of Chromosomes X and 11 and Poor Prognosis in Squamous Cell Carcinomas of the Head and Neck

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