Numerical analysis of SDS–PAGE protein patterns of<i>Serratia marcescens</i>: a comparison with other typing methods

Holmes, B.; Costas, M.; Sloss, L. L.

doi:10.1017/s0950268800047701

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…Biotyping provides a current way for discriminating between nosocomial strains, but only biotypes A5, A8a, A8b, and TCT are predominantly represented in hospital wards (13). Other methods, including serotyping (26), phage typing (9), testing for bacteriocin production or susceptibility (7,8,27,28), and electrophoresis of outer membrane proteins (2) or total proteins (15,20), have been found to be unsatisfactory because of insufficient discrimination and/or poor reproducibility. Restriction endonuclease analysis of DNA with conventional agarose gel electrophoresis has been successfully used for epidemiological fingerprinting.…”

Section: Discussionmentioning

confidence: 99%

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

et al. 1995

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Ribotyping carried out with a nonradioactive probe (acetylaminofluorene ribosomal RNA kit I from Eurogentec, Seraing, Belgium) was performed for the characterization of 139 hospital strains of Serratia marcescens. These strains, which belonged to 11 biotypes and 1 nontypeable group, were isolated in seven hospitals in Belgium between 1986 and 1992. EcoRI and HindIII were used to obtain cleavage patterns. Analysis of the results produced 27 different patterns with EcoRI and 23 patterns with HindIII. Typeability reached 100%. Combination of the patterns obtained with each enzyme produced 38 distinct ribotypes. Percent similarity values, calculated by using the Dice coefficient and unweighted-pair group average linkage clustering, showed four main clusters and nine subclusters of ribopatterns at a similarity rate of approximately 80% or less. These groups did not coincide with those delimited by biotyping, although a rather good correlation was observed. The simultaneous use of the two methods has potential value in epidemiological studies. and ESN, five other hospitals in the Liège area; BXL, Hôpital Universitaire Brugmann, Brussels, Belgium. b ?, month unknown. c RFLP, restriction fragment length morphism.2638 CHETOUI ET AL. J. CLIN. MICROBIOL.

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Section: Discussionmentioning

confidence: 99%

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

et al. 1995

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“…A number of methods have been described for the typing of S. marcescens including serotyping and phage typing [5,6], biotyping [7], bacteriocin typing [8], whole-cell protein fingerprinting [9], multilocus enzyme typing and plasmid profiles [10] and restriction endonuclease analysis of chromosomal DNA [11]. The reference methods of serotyping and phage typing are restricted to a small number of reference centres and the other techniques are most suitable for comparative typing or fingerprinting of isolates within outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification ofSerratia marcescens

Alonso

Aucken²,

Pérez-Díaz

et al. 1993

Epidemiol. Infect.

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SUMMARYVariations in rDNA gene loci in DNA digests of 209 clinical isolates of Serratia marcescens were determined with an Escherichia coli rRNA probe. Forty-one restriction fragment length polymorphism patterns (ribotypes) were identified, based on the size of 4-14 (mean 7.5) hybridization bands. The patterns differed by more than a single band in 98% of pair-wise comparisons. On a subset of 76 isolates, ribotyping proved to be marginally more discriminating than biotyping (discrimination index 0-92 v. 0-89) followed by serotyping (0-87) and bacteriocin typing (0 74). About one-third of isolates belonged to unique ribotypes and only two ribotypes exceeded 5 % in frequency (23-0 and 6-4 % respectively). A combination of serotype or biotype with ribotyping defined a similar number of strains, although none of the methods alone was sufficiently discriminatory to identify strains. We conclude that due to the accessibility of biotyping and the lack of commercially available antisera for S. marcescens, the biotype and ribotype together provide reliable markers of strain identity.

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“…Different types of electrophoretic techniques have been used for the characterization or typing of Candida species including separation of chromosomes, DNA fragments, isoenzymes, cell-wall glycoproteins and whole-cell proteins (5,8,9,28,30,34,45,47,50). In regard to whole-cell proteins, their separation has been employed satisfactorily in the characterization of bacteria and yeast (13,22,26,55,56,57,58). Many investigators employed electrophoretic analysis of whole-cell proteins in the fungi taxonomy (19,21,49,58).…”

Section: Introductionmentioning

confidence: 99%

SDS-Page and numerical analysis of Candida albicans from human oral cavity and other anatomical sites

Rodrigues

Höfling

Boriollo

et al. 2004

Braz. J. Microbiol.

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The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity coefficient of simple matching and UPGMA algorithm, respectively. The results obtained showed several C. albicans subtypes and their similarity degrees (80% to 100%). Such data showed that same patients may be infected by two or more C. albicans subtypes in certain anatomical sites (i.e. only in oral cavity of immunocompromised patients, blood, or tracheal secretion), or yet, two or more patients can be infected in identical anatomical sites (i.e. bronchial washing, urine, oral cavity, tracheal secretion, vaginal secretion, and healthy saliva) with a same C. albicans subtype. However, two or more patients also can show infections in corresponding sites (i.e. oral cavity of immunocompromised patients, blood, oropharyngeal secretion, oral cavity, tracheal secretion, vaginal secretion, and healthy saliva) by different C. albicans subtypes. Besides, two or more patients also can be infected with identical or different C. albicans subtypes in different anatomical sites (i.e.1. identical subtypes in vaginal secretion, tracheal secretion, and urine; abdominal secretion and spittle; drainage and oral cavity; catheter and healthy saliva -i.e.2. subtypes different in bronchial washing, oropharyngeal secretion, pulmonary secretion, oral cavity of immunocompromised patients, and blood). Complementary studies involving C. albicans sample isolated from several anatomical sites of immunocompetent or immunocompromised patients (before, during and after specifics therapies) and their families or hospital workers must be done in order to establish the sources of C. albicans colonization. The whole-cell proteins profile performed by SDS-PAGE associated with computerassisted numerical analysis may provide preliminary criteria for taxonomic and epidemiological studies of such microorganisms.

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Numerical analysis of SDS–PAGE protein patterns ofSerratia marcescens: a comparison with other typing methods

Cited by 14 publications

References 24 publications

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping

Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification ofSerratia marcescens

SDS-Page and numerical analysis of Candida albicans from human oral cavity and other anatomical sites

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