Renewable energy resources are need of the hour at the current energy scarcity scenario in the world. Scientist and researchers are finding the ways to replace the conventional energy resources with the renewable ones. It is fact that fossils are going to be obsolete in future. One third of global energy is being consumed by the transportation sector. All the amount of this energy comes from the fossils that contain the hydrocarbons in their composition. Efforts are being made to replace the fossils with the renewable energy resources. In this regard, biofuels are emerged as a replacement of the diesel fuels. There are several processes in the engine cylinder from atomization of fuel until the exhaust of gases. One of them is the evaporation of fuel droplets. In the present work, evaporation characteristics of conventional diesel fuel and biofuels is described by comparing them in different working conditions. Modeling of evaporation phenomenon using computational fluid dynamics (CFD) techniques and the effects of in cylinder conditions is also explained. Results show that biofuel droplets show a better evaporation rate at the high operating conditions in the engine cylinder.