In this paper, the performance of the turbine blade squealer tip has been studied detailed aimed to highlight the impact of the upstream guide vane passing wake. The first stage of GE-E3 high-pressure turbine has been employed to perform the three-dimensional simulation and the computational domain has been scaled based on the domain scaling method. Boundary conditions are consistent with operating conditions of the annular cascade testing. Circumferential averaged and realistic non-uniform interface conditions have been used to obtain steady and unsteady characteristics respectively. The validation of the turbulent model and mesh independent test has been conducted detailed in previous work. Three squealer tips, including two widths and heights, have been designed and investigated to understand its influence. Results show that the aerothermal performance of the squealer tip is remarkably influenced by the upstream passing wake. Although steady and time-averaged results have a good agreement, the variation of instantaneous heat transfer coefficient (HTC) would be over 30%, especially on the Cavity Floor region. Changing the geometry of the squealer also has different impacts on both steady and unsteady performance. The unsteady aerodynamic has relatively small fluctuation within 10%, and the distribution of steady and time-averaged leakage flow as well as total pressure loss coefficient still have a satisfactory agreement.