Solute transport characteristics and groundwater connection structures are mainly studied in this paper by adopting the numerical simulation and field tracer test. Firstly, the simulation method of solute transport is proposed. Then two kinds of representative geological models of tracer test are built, and the process of solute transport is simulated. The variation characteristics of solute transport under the conditions of straight pipeline and branch pipeline are summarized by analysis of the simulation results. The effects of the pipeline width, flow velocity and path difference of branch pipelines on the tracer curve are discussed. General laws of groundwater connection tracer curve are obtained. Finally, based on the field test results, and combined with engineering geological conditions, characteristics of karst groundwater systems, the groundwater connection structures are analysed and speculated in detail by the flow velocity and local monitoring curve. The results show that: (i) As the flow velocity increases, the peak of the tracer curve decreases gradually, as well as the time to peak; (ii) The peak of the tracer curve gradually decreases with the increasing pipeline width; (iii) Under the different path difference of branch pipeline, the tracer curves all present obvious ‘trailing’ phenomenon; (iv) The analysis process of groundwater connection structures can provide references for the same type of hydrological geological problems.