The quality of carbon steels working at variable loads mainly depend of microstructure, but also of impurities. The quantity and morphology of non-metallic inclusions and spaces between impurities are correlated with the content of admixtures in the alloy, while their phase composition and structure, in particular shape, dimensions and dispersion, are determined by the course of metallurgical processes. Non-metallic inclusions as impurities found in steel can affect its performance characteristics. Their impact depends not only on their quality, but also, among others, on their size and distribution in the steel volume. The literature mainly describes the results of tests on hard steels. The article discusses the results of a study investigating the effect of the number of large non-metallic inclusions (over 10 μm in size) on the fatigue strength of structural steel during rotary bending. The study was performed on 6 heats produced in an industrial plant. Fourteen heats were produced in 140 ton electric furnaces. All heats were desulfurized and refined with argon. The experimental variants were compared in view of the tempering the research steel. The fatigue strength of steel with an impurity spaces was determined during rotary bending: the results revealed that fatigue strength is determined by the impurity spaces and tempering temperature.