Numerical Taxonomy of Vibrionaceae Isolated from Oysters and Seawater Along an Annual Cycle

Ortigosa, Margarita; Garay, Esperanza; Pujalte, María J.

doi:10.1016/s0723-2020(11)80011-1

Cited by 65 publications

(51 citation statements)

References 22 publications

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“…The dominant bacteria found in the water were also found in previous analyses of the same area (Ortigosa et al 1994a,b, Arias et al 1999. The only study specifically devoted to the analysis of the bacterial content of Sparus aurata larvae, performed by Grisez et al (1997), found no dominant or persistent colonisation of the intestines of the larvae up to 30 d, and reported that fluctuations in the composition of the dominant microflora were closely related to the bacterial contents of the ingested food.…”

Section: Discussionsupporting

confidence: 80%

“…These species have been recovered from moribund or recently dead fish showing clinical signs. However, studies on the bacterial flora present in asymptomatic fish are scarce.Several studies have been conducted along the Spanish Mediterranean coast to identify the main bacterial groups present in water and bivalves (Ortigosa et al 1994a,b, Arias et al 1999. Some of these bacteria exhibit clear seasonal variation, as Vibrio harveyi dominated with temperatures above 20°C and V. splendidus dominated at temperatures below 20°C (Arias et al 1999).…”

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confidence: 99%

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Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

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A bacteriological survey of gilthead sea bream Sparus aurata from different fish farms and culture systems on the Spanish Mediterranean coast was conducted. Three different studies were performed. Study A included hatchery-reared larvae; Study B, periodic examination of randomly sampled growing fish; and Study C, growing fish sampled only during mortality/morbidity events. In Studies B and C, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical signs) and non-diseased fish were included. In Study A, a shift from Vibrio spp. (30 d after hatching) to oxidative species (60 d after hatching) was detected, and no mortality events were registered. The percentage of fish yielding bacterial growth were similar in Studies B and C, reaching 57.4 and 61.3%, respectively. A statistically significant relationship between the bacterial carriage and the type of facility was only found in Study B, showing that fish from sea cages had a higher bacterial occurrence than fish from other facilities. A statistically significant relationship between bacterial carriage and signs of disease was found, although the pattern differed in each study. Thus, in Study B only 36.2% of fish yielding abundant bacterial growth were diseased, versus 68.0% in Study C. In total, 25.0% of the fish examined were diseased. Bacterial species composition was similar in asymptomatic and diseased fish, except for a group of V. ichthyoenteri-like isolates that occurred almost exclusively in asymptomatic fish. Dominant bacterial species were V. harveyi and V. splendidus, followed by V. ichthyoenteri-like isolates, Photobacterium damselae ssp. damselae and V. fisheri. Non-fermenters were less frequent but, among them, unidentified halophilic Cytophaga-Flavobacterium isolates and Pseudoalteromonas haloplanktis were the most abundant. An association of individual species with disease was not clear, which suggests the involvement of mixed infections. KEY WORDS: Sparus aurata · Vibrio harveyi · Vibrio splendidus · Photobacterium damsela · Pseudoalteromonas haloplanktis · Vibrio ichthyoenteri · Mediterranean aquaculture Resale or republication not permitted without written consent of the publisherDis Aquat Org 54: [119][120][121][122][123][124][125][126] 2003 Baptista et al. 1999). These species have been recovered from moribund or recently dead fish showing clinical signs. However, studies on the bacterial flora present in asymptomatic fish are scarce.Several studies have been conducted along the Spanish Mediterranean coast to identify the main bacterial groups present in water and bivalves (Ortigosa et al. 1994a,b, Arias et al. 1999. Some of these bacteria exhibit clear seasonal variation, as Vibrio harveyi dominated with temperatures above 20°C and V. splendidus dominated at temperatures below 20°C (Arias et al. 1999). These and other bacterial species showing seasonal occurrence have been reported as opportunistic pathogens for sea bass and sea bream (Balebona 1998b).In the present study, bacterial car...

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Section: Discussionsupporting

confidence: 80%

mentioning

confidence: 99%

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

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“…Among V. harveyi strains, 23% had swarming motility, 47% had resistance to a 8% sodium chloride solution, 52% were positive for lysine decarboxylase, and 47% were positive for ornithine decarboxylase. In addition, they have been reported to have divergent phenotypic traits 22) . The pathogenic vibrios isolated in this study might be classified into Phenon 7 due to the swarming motility defined by Ortigosa et al 22) We tested the pathogenicity of the representative swarming V. harveyi strain S20 for Ezo abalone.…”

Section: 4×10mentioning

confidence: 99%

“…In addition, they have been reported to have divergent phenotypic traits 22) . The pathogenic vibrios isolated in this study might be classified into Phenon 7 due to the swarming motility defined by Ortigosa et al 22) We tested the pathogenicity of the representative swarming V. harveyi strain S20 for Ezo abalone. Figure 4 shows the results of immersion and injection trials of the candidate bacterial strain.…”

Section: 4×10mentioning

confidence: 99%

Mass Mortality of Japanese Abalone Haliotis discus hannai Caused by Vibrio harveyi Infection

et al. 2007

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Sixty thousand of deaths among cultured Ezo abalone Haliotis discus hannai occurred within a few days at an abalone farm in Japan in the middle of August, 2002. Dead animals were characterized by a hemolymphatic edema around the major circulatory system. Vibrios showing swarming motility dominated in the edema. The pathogenic vibrios were identified as Vibrio harveyi based on a phylogenetic analysis and a phenotypic characterization. In both immersion and injection experiments, the swarming vibrios fulfilled Koch's postulates as a pathogen for Ezo abalone. Using a GFP-tagged V. harveyi S20, a clump of bacterium was detected on the gills of the abalone within 48 hours after contact with the bacterium. This is the first report of V. harveyi infection in Ezo abalone Haliotis discus hannai.

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“…De todos os grupos de microrganismos estudados no ambiente marinho, os membros da família Vibrionaceae, provavelmente, constituem a maior parte das populações bacterianas heterotróficas (ORTIGOSA et al, 1994;HEIDELBERG, 1997). Esta família é composta por 8 gêneros, sendo o gênero Vibrio o maior deles, compreendendo 81 espécies descritas que são ubíquas do ambiente marinho (THOMPSON e SWINGS, 2006;THOMPSON et al, 2007).…”

Section: Seqüenciamento Parcial Da Região 16s Rrna Dos Isolados Bacteunclassified

Diversidade de bactérias em amostras de água do mar no canal de São Sebastião

Almeida¹

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Numerical Taxonomy of Vibrionaceae Isolated from Oysters and Seawater Along an Annual Cycle

Cited by 65 publications

References 22 publications

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Mass Mortality of Japanese Abalone Haliotis discus hannai Caused by Vibrio harveyi Infection

Diversidade de bactérias em amostras de água do mar no canal de São Sebastião

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