Numerical Taxonomy of Vibrios Isolated from Estuarine Environments

Kaper, James B.; Lockman, Hank; Remmers, Elaine F.; KRISTENSEN, K.; Colwell, R. R.

doi:10.1099/00207713-33-2-229

Cited by 23 publications

(11 citation statements)

References 16 publications

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“…Vibrio furnissii produces acid from L-arabinose and differs from our new species in its responses in many nutritional tests (6, 30). Vibrio marinus cannot grow at 37°C or in the presence of 7% (wt/vol) NaC1, cannot hydrolyze starch, and cannot produce acid from mannitol(l5, 18,30). Vibrio splendidus I is luminescent, and Vibrio spendidus I1 cannot grow at 37°C (2,29,30).…”

Section: Vibriomentioning

confidence: 99%

Vibrio navarrensis sp. nov., a Species from Sewage

Urdaci

Marchand²,

Ageron³

et al. 1991

International Journal of Systematic Bacteriology

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A group of 11 strains, mostly isolated from sewage water in the Province of Navarra, Spain, were found to constitute a DNA relatedness group which is 2 to 39% related to 23 species of the genus Vibrio and 2 to 3% related to two Aeromonas species. Phenotypically, these strains have all of the properties that define the genus Vibrio. However, they differ from the previously described species by three or more properties. The strains are negative for arginine, ornithine, and lysine decarboxylase activities and the Voges-Proskauer test and are unable to utilize putrescine, gluconate, glucuronate, and histidine. They utilize and produce acid from sucrose and grow at 40°C. All strains grow in the presence of 0.5% (wthol) NaCl, and seven strains grow weakly in peptone water lacking NaCl. The group of strains which we studied can also be differentiated from other Vibrio species by fatty acid content. The G+C ratio of the DNA is 45 to 47 mol%. The name Vibrio navarrensis sp. nov. is proposed for these strains; strain 1397-6 (= CIP 103381) is the type strain.

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Section: Vibriomentioning

confidence: 99%

Vibrio navarrensis sp. nov., a Species from Sewage

Urdaci

Marchand²,

Ageron³

et al. 1991

International Journal of Systematic Bacteriology

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“…In addition to the 22 Vibrio species described in Bergey's Manual of Systematic Bacteriology (2), 14 new species have been recognized (3,4, 7, 9,15,17,18,20,24,31,33,36,37,41)-Phenotypic characterization data for many fresh isolates indicate that it is often difficult to correctly identify these organisms to species level and suggest that a number of isolates might correspond to undescribed species (6, 21,28,39,40).…”

mentioning

confidence: 99%

Vibrio mytili sp. nov., from Mussels

Pujalte

Ortigosa

Urdaci

et al. 1993

International Journal of Systematic Bacteriology

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Five strains isolated from mussels (harvested off the Atlantic Spanish coast in 1985 and 1986) were found to be phenotypically distinct from previously described Vibrio species, They showed 94 to 100% intragroup relatedness as determined by DNA-DNA hybridization (S1 nuclease method) but were found to be only 1 to 25% related to other Vibrio species. These strains have all of the properties that define the genus Vibrio and can be clearly differentiated from other species by their positive responses in tests for Thornley's arginine dihydrolase, gas production from glucose, growth in media containing 10% NaCl, and acid production from sucrose, L-arabinose, D-xylose, and D-cellobiose and their negative responses in tests for lysine decarboxylase, the Voges-Proskauer reaction, growth without NaCl and at 40°C, hydrolysis of gelatin, casein, starch, DNA, and alginate, and acid production from D-mannose. The G+C ratio of the DNA is 45 to 46 mol%. The name Vibriu mytili is proposed for the new species; strain 165 (= CECT 632) is the type strain.The genus Ebrio currently contains more than 35 species, most of which are of marine origin. In addition to the 22 Vibrio species described in Bergey's Manual of Systematic Bacteriology (2), 14 new species have been recognized (3,4, 7, 9,15,17,18,20,24,31,33,36,37,41)-Phenotypic characterization data for many fresh isolates indicate that it is often difficult to correctly identify these organisms to species level and suggest that a number of isolates might correspond to undescribed species (6,21,28,39,40).It is generally agreed that a bacterial species is a group of strains with high levels of intragroup DNA relatedness which can also be recognized by phenotypic traits (38). There is no such operational definition of a genus at the present time. Although phylogenetic criteria are of tremendous importance in delineating a genus (and higher taxa), it has been agreed that genera should contain species with common phenotypic traits to allow phenotypic identification at the genus level (26). Although splitting of the genus Vibrio into Hbrio sensu strict0 and the genus Listonella on the basis of molecular data has been proposed (25), there is not general agreement about the constituent species of the genus Listonella (10,35) or the phylogenetic justification of this proposed genus (8,27), and most workers still refer to Listonella spp. as Ebrio spp. for phenotypic reasons.Five strains isolated from mussels could not be identified as any known Vibrio species (28). The purpose of this work was to examine the taxonomic position of these strains relative to known species by DNA-DNA hybridization and extensive phenotypic tests. The outcome is the description of a new species named Vibrio mytili. MATERIALS AND METHODSBacterial strains. I? mytili 163, 165= (T = type strain), 167, 178, and 185, the strains that constituted phenon 3 (28), were isolated from Mytilus edulis and were maintained as working stocks at room temperature in semisolid seawater agar containing 1% (wt/vol) peptone, 0.3% mea...

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“…Taxonomic studies (2, 6,18,29,34) of genetic, biochemical, and physiological data clearly distinguish V. parahaemolyticus from V. alginolyticus, with differential taxonomic characteristics shown for isolates from geographically diverse areas. Here we report the results of analyses of taxonomic, ecological, pathogenic, and serologic data for 567 V. parahaemolyticus and V. alginolyticus isolates from seawater samples collected in Jakarta Bay and from seafood samples taken from markets in Jakarta, Indonesia.…”

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confidence: 99%

Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia

Molitoris¹,

Joseph²,

Krichevsky³

et al. 1985

Appl Environ Microbiol

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Previous studies have shown that Vibrio alginolyticus and Vibrio parahaemolyticus can be isolated from similar types of marine samples. In this report, the results of an examination of 567 V. alginolyticus and V. parahaemolyticus strains, isolated from seawater in Jakarta Bay and from more than 30 types of seafood from markets in Jakarta, Indonesia, are presented. Most isolates were from mackerel, shrimp, or squid. Numerical taxonomic analyses clustered 337 isolates and three V. alginolyticus reference strains at S 2 80%. These strains produced acid from sucrose, but only approximately 80% produced acetoin or grew in the presence of 10% NaCl. The frequency of occurrence of V. alginolyticus in seawater samples ranged from 0% (in February and March 1972) to 100% (in September and December 1972) and was highest in seafood samples from August to December 1972. A second cluster of 230 isolates and seven V. parahaemolyticus reference strains was observed at S-82%. These strains did not produce acetoin or acid from sucrose, and approximately 20% grew in the presence of 10% NaCI. V. parahaemolyticus was detected in seawater samples each month, with the highest frequency of occurrence (83.3%) in May 1972. Twenty-nine K antigen serotypes were demonstrated in V. parahaemolyticus isolates, and another 40% were untypable. The modal antibiotic resistance pattern for each species included five drugs. Only 12% of the V. parahaemolyticus strains were Kanagawa positive, and 10% elicited fluid accumulation in ligated rabbit ileal loops. All of the 7 V. alginolyticus strains and 94 (70%) of the V. parahaemolyticus strains tested killed mice when inoculated intraperitoneally. Both V. alginolyticus and V. parahaemolyticus were concluded to be indigenous to seawater and were frequently isolated from seafood in Jakarta, Indonesia. For this reason they should be of concern to clinicians and health authorities in this and other tropical areas.

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Numerical Taxonomy of Vibrios Isolated from Estuarine Environments

Cited by 23 publications

References 16 publications

Vibrio navarrensis sp. nov., a Species from Sewage

Vibrio navarrensis sp. nov., a Species from Sewage

Vibrio mytili sp. nov., from Mussels

Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia

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