Patients requiring continuous mechanical ventilation benefit from easier airway management through tracheostomy, a standard treatment in critical care. However, gaps in nurses’ comprehension of tracheostomy car can lead to complications such as infections and airway blockages. Therefore, understanding nurses’ competency in tracheostomy care is essential for enhancing patient outcomes in intensive care units (ICUs). A cross-sectional study was conducted in the southern West Bank of Palestine among 237 nurses working in ICUs to assess their level of knowledge in tracheostomy care. Data were gathered via a structured questionnaire and analyzed using inferential tests and descriptive. Most nurses showed moderate knowledge of tracheostomy care, with significant differences observed based on age, gender, years of experience, and educational level. To significantly improve patient outcomes and safety in ICUs, it is imperative that nurses participate in continuous education and training programs focused on tracheostomy care.