Nutraceutical properties of dietary plants extracts: Prevention of diabetic nephropathy through inhibition of glycation and toxicity to erythrocytes and HEK293 cells

Abstract

Context: Glycated albumin is reported to elicit pathobiologic effects in diabetic nephropathy and abrogating its biologic effects has novel therapeutic potential. Objective: This study examines the effects of dietary plants extracts (Laurus nobilis, Carum carvi, Coccinia grandis, Mentha arvensis, Phaseolus vulgaris) against albumin glycation and its toxicity to erythrocytes and HEK293 cells. Materials and methods: Albumin (10 mg/ml) was incubated with fructose (250 mM) in PBS along with aqueous plant extracts (1% w/v) for 4 d. After incubation, the antiglycation potential of extracts was estimated by measuring AGEs, fructosamine, amyloids, carbonyls, free amino groups, and antioxidant potential of albumin. The glycation extent of the treated samples was determined by boronate affinity chromatography. Effect of extracts against glycation induced cytotoxicity in erythrocytes and HEK 293 cells was assessed by estimating viability, glutathione, and antioxidant capacity. Plant extracts were tested for their phenolic content and antioxidant potential (reducing potential, DPPH, ABTS, NO, and H 2 O 2 radical scavenging activities). Results: Plant extracts significantly decreased the AGEs formation and amyloid aggregation in glycated BSA (p50.001). Further, fructosamine and carbonyls were reduced to 55-72% and 83-89%, respectively. Free amino group and antioxidant activity of albumin were also preserved by 1.25-1.40-fold and 1.75-1.8-fold, respectively. Further, co-incubation of extracts with glycated albumin, protected erythrocytes, and HEK293 cells as they inhibited cellular hemolysis/toxicity (p50.001) by upregulating cellular antioxidants. Discussion and conclusion: Plant co-incubation reversed many modifications in albumin glycation, cellular dysfunction indicating that dietary sources with antiglycating and antioxidant potential could be considered for the effective management of diabetic nephropathy.

Antioxidant, antiglycation and insulinotrophic properties of Coccinia grandis (L.) in vitro: Possible role in prevention of diabetic complications

“…It was observed that CGF extract showed significant inhibition to amyloid aggregation during the course of experiment, 7–28 days, indicating their potential to prevent conformational changes in albumin during glycation. The reason behind decrease in β-amyloid formation may be the low-molecular-weight bioactive compounds such as polyphenols, phenolic acids or flavonoids present in the extract which have anti-amyloidogenic effects 49 . The results of this study corroborate the effective antiglycation action of CGF extract as they not only counteracted with the initial glycation reaction and its progression but also prevented amyloid cross β-structures in BSA in vitro .…”

Inhibition of glycation-induced cytotoxicity, protein glycation, and activity of proteolytic enzymes by extract from Perovskia atriplicifolia Roots

“…In our study, we only tested the survival rate of cells, but from the results obtained by Tupe et al . [ 24 ] we can presume that the other parameters are likely to correspond to the previous study.…”

“…Its antiglycative properties were confirmed previously in several reports on such medicinal herbs as Salvia sp., Melissa officinalis, Mentha spicata , and other Lamiaceae. [ 23 24 25 ] The numerous other bioactivities have been also reviewed. [ 1 26 ] However, the ability of a rosmarinic acid-rich extract to protect mammalian cells against damage induced by glycated albumin has not been reported, yet.…”

“…On the other hand, we obtained similar results using four different mammalian lines to prove the more universal than cell-line specific activity of tested extract. In the study of Tupe et al ,[ 24 ] using HEK293 and red blood cells, some other plant extracts have been also proved to be efficient protective agents. One of the extracts in the above cited report was from Mentha arvensis , but these authors did not mention rosmarinic acid as one of the active components.…”

Attenuation of glycation-induced multiple protein modifications by Indian antidiabetic plant extracts

“…Functional molecules that are present in natural compounds are responsible for their curative potential. Culinary herbs with high polyphenol content and antioxidant activity represent promising approach (Tupe et al 2013b(Tupe et al , 2015b. Recent studies have shown the beneficial effects of medicinal plants and bioactive compounds in AGEs mediated pathogenesis using experimental diabetic animal models (Elosta et al 2012;Sadowska-Bartosz & Bartosz 2015;Younus & Anwar 2016).…”