Nutrient utilization in liquid/membrane system for watermelon micropropagation

Abstract

Watermelon [Citrullus lanatus (Thunberg) Matsumura and Nakai] proliferating shoot meristems from established shoot cultures were inoculated on modified Murashige and Skoog salts medium supplemented with 10 txM 6-benzyladenine (BA) for shoot proliferation and on similar medium supplemented with 1 IxM BA and 10 txM gibberellic acid (GA3) for shoot elongation. Agar-solidified medium and microporous polypropylene membrane rafts in liquid medium were used to support the tissues. Growth over culture time of proliferating and elongating tissues in liquid and agar-solidified media were compared. Nutrient depletion in liquid medium was monitored and quantified using ion selective electrodes. Tissue fresh weights in both proliferation and shoot elongation media were greater in liquid than in agar-solidified medium. Relative dry matter content, however, was greater in agar-solidified than in liquid medium. More shoots elongated in agar-solidified than in liquid medium. The numbers of buds or unelongated shoot meristems, however, were comparable for both the liquid and agar-solidified medium. Proliferating and elongating tissues in liquid medium used Ca ++ and K ÷ minimally. NO 3 was utilized but not depleted by proliferating tissues. NH4, however, was depleted. Most of the NH 4 was utilized by the proliferating tissues within 21 days of culture when growth rate was greatest. At 35 days, residual Ca ++, K +, NO 3, and NH2 in proliferation medium were 81.0%, 67.8%, 55.7%, and 1.2% of initial levels, respectively. NO 3 and NH 4 in shoot elongation medium were depleted. The greatest NO 3 and NH 4 utilization was observed during the first 14 days of culture Ca , NO3 and NH4 in shoot elongation when the largest growth rate was obtained. The residual + ÷ K ÷ , , medium at 38 days were 63.5%, 37.9%, 21.2%, and 24.3% of initial concentrations, respectively. At the end of experiment, 72.3% and 42.8% of initial sugars were still remaining in the shoot proliferation and shoot elongation medium, respectively.

Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts

“…Plant micropropagation systems on liquid medium associated to membrane rafts has been successfully used in several works (Watad et al, 1995;Desamero et al, 1993;Adelberg et al, 1992). However, as far as we know this is the first report describing the use of such system for somatic embryogenesis induction.…”

“…On plant micropropagation studies, this culture system has shown better results when compared to cultures conducted on either liquid and solid medium (Hew et al, 1990;Young et al, 1991;Desamero et al, 1993;Watad et al, 1995). However, it has never been tested in studies on induction and regeneration of somatic embryos.…”

Utilização de nutrientes por células de algodoeiro cultivadas em suspensão

“…Os estudos sobre os requerimentos nutricionais de plantas in vitro visam otimizar o crescimento das culturas, como também relacionar a taxa de crescimento com a absorção dos elementos do meio (Desamero et al, 1993 ) seria razoável supor que meios de cultura com concentrações menores sejam suficientes para sustentar o crescimento celular.…”

Caracterización y extracción de citrulina de la corteza de la sandía (Citrullus lanatus “thunb”) consumida en Valledupar

“…(Al-Sayed y Ahmend 2013), vitaminas (Desamero et al 1993), micronutrientes esenciales y citrulina El principal interés científico de la citrulina es que se encuentra presente en tres vías metabólicas diferentes: como intermediario del ciclo de la urea en el hígado, como productor de la enzima sintasa (necesaria en la producción de óxido nítrico) y como sintetizador da Arginina (Curis et al 2005;Romero et al 2006). Además la citrulina tiene gran poder antioxidante (Fang et al 2002), siendo eficiente eliminador de radicales hidroxilos (Akashi et al 2001), y se utilizada como relajante y como vasodilatador para reducir la fatiga y dolores musculares producido por el ácido láctico después del esfuerzo físico (Tarazona et al 2011), y tiene función nutricional para los ancianos (Piñeiro et al 2010), dentro de otras propiedades benéficas a la salud.…”