The main study focuses on the extraction of chitosan from chitin isolated from the exoskeleton of a squid pen and the synthesis of silver chitosan nanoparticles. Chitosan is an amino polysaccharide prepared by processing the squid pen which involves demineralization, deproteinization, and deacetylation. Chitosan is a versatile natural polysaccharide, its second most abundant natural polymer. Previous studies have found that chitosan is biocompatible, biodegradable, and non-toxic, which has wide applicability in the pharmaceutical field, food industries, drug delivery, cancer treatment, and biological imaging and diagnosis. The chitosan and silver chitosan nanoparticles yield was found to be 25% and 51.25%. The conformation of silver chitosan nanoparticles was done through UV spectrum, FTIR, and XRD. Keywords: Chitosan, Chitin, Biopolymer, Characterization, UV spectrum, FTIR, XRD.