Nutrition, Nutritional Diseases, and Nutrition Research Applications

Navia, Juan M.; Hunt, Charles E.

doi:10.1016/b978-0-12-730050-4.50022-3

Cited by 40 publications

(29 citation statements)

References 189 publications

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“…The primary factor determining the vitamin C requirement for guinea pigs is the prevention of scurvy [16]. Dietary ascorbate levels as low as 0.2 g/kg diet are effective while higher dietary levels have no adverse effects and will result in higher tissue concentrations [17].…”

Section: Methodsmentioning

confidence: 99%

Combined selenium and vitamin C deficiency causes cell death in guinea pig skeletal muscle

et al. 2009

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Combined antioxidant deficiencies of selenium and vitamin E or vitamin E and vitamin C in guinea pigs result in clinical illness. We hypothesized that combined selenium and vitamin C deficiency would have clinical consequences because in vitro interactions of these antioxidant nutrients have been reported. Since guinea pigs are dependent on dietary vitamin C, weanling male guinea pigs were fed selenium-deficient or control diet for 15 weeks prior to imposing vitamin C deficiency. Four dietary groups were formed and studied 3 weeks later: controls, vitamin C deficient, selenium deficient, and doubly deficient. Deficiencies were confirmed by determinations of glutathione peroxidase activity and vitamin C concentration in liver and skeletal muscle. Plasma creatine phosphokinase (CPK) activity and liver, kidney, heart, and quadriceps histopathology were determined. Doubly deficient animals had moderately severe skeletal muscle cell death as judged by histopathology and plasma CPK activity of 6630 ± 4400 IU/L (control 70 ± 5; vitamin C deficient 95 ± 110; selenium deficient 280 ± 250). Liver, kidney, and heart histology was normal in all groups. Muscle α-tocopherol levels were not depressed in the doubly deficient group but muscle F 2 isoprostane concentrations were elevated in them and correlated with markers of cell death. We conclude that combining selenium and vitamin C deficiencies in the guinea pig causes cell death in skeletal muscle that is more severe than the injury caused by selenium deficiency. The elevation of muscle F 2 isoprostanes is compatible with the cell death being caused by oxidative stress.

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Section: Methodsmentioning

confidence: 99%

Combined selenium and vitamin C deficiency causes cell death in guinea pig skeletal muscle

et al. 2009

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“…Although some disparities with the normal diet were found, nutrient levels were adequate (Navia and Hunt, 1976). It is recognized that after 14 days of vitamin C deficiency guinea pigs become anorexic (Peterkofsky et al, 1986).…”

Section: Nutritional Status Of Enzyme-treated Animalsmentioning

confidence: 95%

A protocol for the successful long‐term enzyme replacement therapy of scurvy in guinea pigs

Hadley

Sato

1988

J of Inher Metab Disea

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Gulonolactone oxidase, a key enzyme in the biosynthesis of ascorbic acid, is missing from guinea pigs and certain other scurvy-prone species. Weekly intraperitoneal injections of glutaraldehyde cross-linked immunoprecipitates of this enzyme have been shown to provide guinea pigs with the capability of synthesizing their own ascorbic acid and of surviving without an exogenous source of this vitamin. This protocol, however, was successful in only a small percentage of the animals tested. The reasons for the limited therapeutic success were investigated. Apparently, the gulonolactone oxidase-treated guinea pigs fed without ascorbic acid were receiving insufficient nutrition. By supplementing these enzyme-treated animals with vitamins A, B, D and E and selenium, we successfully maintained a high proportion of guinea pigs fed without vitamin C.

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“…Navia & Hunt (1976) reported that nephritis was a fairly common finding in older guineapigs, possibly associated with nutritional deficiencies, but did not mention an association with cystitis or urinary stones.…”

Section: Discussionmentioning

confidence: 99%