Priatni S, Ratnaningrum D, Kosasih W, Sriendah E, Srikandace Y, Rosmalina T, Pudjiraharti S. 2018. Protein and fatty acidprofile of marine fishes from Java Sea, Indonesia. Biodiversitas 19: 1737-1742. Indonesia is the second largest producer of capturefisheries products in the world and the most capture fisheries production comes from marine fisheries. Marine fish is a source of protein,amino acid, saturated and unsaturated fatty acids, which are important components of diet. The objective of the study was to investigatethe protein and fatty acids profile of nine marine fish samples from Java Sea of Indramayu West Java, Indonesia. The analysis datashowed that the total protein content of fish samples ranged from 61.07% (Pampus argenteus) to 86.56% (Tetraodontidae). Meanwhile,total lipid content of fish samples ranged from 1.73% (Tetraodontidae) to 9.82% (Leiognathus equulus). The concentration of α-AminoNitrogen (AN) of fish protein hydrolysate was ranging from 31 mM (Nemipterus hexodon) to 69 mM (Mystacoleucus padangensis)and% Degree of Hydrolysis (DH) was ranging from 9.33% to 20.39%. The molecular weight of protein fish samples had similar profilesprimarily for almost all samples, which could be observed from a typical band with the weight around 49 kDa. The saturated fatty acid(Ʃ SFA) compositions of fish species ranged from 1094.03-4233.03 μg/g. Oleic acid (MUFA) content of all fish species ranged from257.91-1216.06 μg/g. However, only three fish species contain of Poly Unsaturated Fatty Acid (PUFA) linoleic acid as the following;Selaroides leptolepis (171.36 μg/g), Oxyeleotris marmorata (249.40μg/g) and Tetraodontidae (140.35 μg/g). The highest SFA contentwas found in S. leptolepis with palmitic acid (C16:0) as the dominant saturated fatty acid (2320.88 μg/g). S. leptolepis also containedhigh oleic acid (1216.06 μg/g) and linoleic acid (171.36 μg/g).