Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene

Freire, Naiana Braga; Magalhães, Thaís Correia; Soares, Riani Ananda Nunes; Costa, Mateus Matiuzzi da; Gouveia, Gisele Veneroni

doi:10.1016/j.micpath.2018.11.044

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…In this study, the adhesion detection on EPCs showed that deletion of the aodp gene increased the ability of the strain to adhere to cells, which was significantly different from the wild strain A. veronii TH0426 ( p < .05). The experiment results were consistent with the above theory and indicated that bacterial adhesion was necessary for biofilm formation (Desbois et al., 2020; Dias et al., 2018; Freire et al., 2019). The LD 50 tests of zebrafish and mice showed a significant increase in LD 50 values and a notable decrease in the virulence of the △‐ aodp strain compared with the wild strain ( p < .01).…”

Section: Discussionsupporting

confidence: 85%

Aeromonas veronii virulence and adhesion attenuation mediated by the gene aodp

Zhang

Jin

Feng

et al. 2021

Journal of Fish Diseases

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Aeromonas veronii (A. veronii) is an opportunistic pathogen of fish–human–livestock, which poses a threat to the development of aquaculture. Based on our previous studies on proteomics and genomics, we found out that the aodp gene may be related to the virulence of A. veronii TH0426. However, aodp gene encodes a hypothetical protein with an unknown function, and its role in A. veronii TH0426 is not clear. Here, we first constructed a mutant strain (△‐aodp) to investigate the functional role of aodp in A. veronii TH0426. Compared with the wild strain A. veronii TH0426, the growth rate of strain △‐aodp was slower and was resistant to neomycin and kanamycin, but sensitive to cephalexin. The swimming and swarming ability of △‐aodp strain decreased, and the pathogenicity to mice decreased by 15.84‐fold. Besides, the activity of caspase‐3 in EPCs infected with △‐aodp strain was 1.49‐fold lower than that of the wild strain. We examined 20 factors closely related to A. veronii virulence, among them 17 genes were down‐regulated as a result of aodp deficiency. This study laid a foundation for further studies on the pathogenesis of A. veronii.

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Section: Discussionsupporting

confidence: 85%

Aeromonas veronii virulence and adhesion attenuation mediated by the gene aodp

Zhang

Jin

Feng

et al. 2021

Journal of Fish Diseases

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“…TapA of Aeromonas salmonicida participates in the process of infecting Atlantic salmon (36). The fla is involved in the formation of Aeromonas spp biofilms and has potential pathogenicity (37). In this study, virulence genes aer, act, gcaT, tapA and fla were detected from strain 18BJ181, but the more complex mechanism of toxicity action of A. veronii still needs to be further explored.…”

Section: Discussionmentioning

confidence: 82%

A First Report of Aeromonas veronii Infection of the Sea Bass, Lateolabrax maculatus in China

et al. 2021

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The sea bass, Lateolabrax maculatus is commercially farmed in Zhuhai, located in the Guangdong Province of China. L. maculatus in aquaculture have suffered acute death, characterized by ulcerations on the body surface, congestion, and hemorrhage in internal organs such as liver, kidney, and spleen. The dominant infecting strain of bacteria isolated from the kidneys of diseased fish was identified as Aeromonas veronii (strain 18BJ181). This identification was based on analysis of morphological, physiological, and biochemical features, as well as 16S rRNA and gyrB gene sequences. Drug sensitivity testing showed that the strain 18BJ181 isolate was resistant to four antibacterial drugs, including amoxicillin, madinomycin, penicillin and sulfamethoxazole, while moderately sensitive to erythromycin and rifampicin. The detection of growth characteristics showed that the strain 18BJ181 exhibited adaptability to the environment. In addition, some virulence genes, such as aer, act, gcaT, tapA and fla, were detected in the strain 18BJ181. The median lethal dosage of the strain 18BJ181 isolate in L. maculatus was 8.5 × 105 and 4.2 × 105 cfu/g under the conditions of intraperitoneal injection and intramuscular injection, respectively. The experimentally induced infection showed that the 18BJ181 isolate caused considerable histological lesions in L. maculatus, including tissue degeneration, necrosis, and different degrees of hemorrhage. These results provided evidence for a more comprehensive understanding of A. veronii strain 18BJ181 infection in L. maculatus.

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“…The bacterial isolates were obtained from the tissues (kidney, integument, intestine, and lesions) of diseased Nile tilapia ( Oreochromis niloticus ). The molecular identification of the isolates as A , hydrophila was determined by 16SrDNA PCR and sequencing previously performed by Freire et al (2019).…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

Silva

Pires

Costa

et al. 2022

Journal of Applied Microbiology

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Aims Aeromonas hydrophila is an opportunistic bacterium, with a high capacity for biofilm production, which can cause severe damage in aquaculture. The objective of this study was to identify the chemical compounds of the essential oils of Lippia sidoides (EOLS) and Cymbopogon citratus (EOCC), and to evaluate the biocidal, antibiofilm and synergistic action with the antimicrobial florfenicol of these essential oils (EOs) against A. hydrophila. Methods and Results The antibacterial activity of EOLS and EOCC was verified by the minimum bactericidal concentration and by the action of these EOs against both forming and consolidated biofilms. The synergistic activity of EOs with florfenicol was performed using the checkerboard technique. The main component of EOLS and EOCC was carvacrol (44.50%) and α‐citral (73.56%), respectively. Both EOs showed weak inhibitory activity (≥3125.00 µg ml−1). Two bacterial isolates were able to produce biofilm, and EOLS and EOCC acted upon the bacterial isolates to prevent biofilm formation. A bactericidal effect was verified for EOLS in the previously consolidated biofilm for both isolates and for EOCC in only one of the isolates. In general, EOLS had a synergistic effect with florfenicol, while EOCF had an additive effect. Conclusions Both EOs were able to interfere with biofilm formation and did not have an antagonistic effect in combination with florfenicol. The best results were found for EOLS, which showed a synergistic effect with florfenicol and the ability to interfere in the formation of consolidated biofilm. Significance and Impact of the Study This study highlights the potential of EOLS and EOCC to interfere in biofilm and act in synergy with florfenicol to reduce the occurrence of A. hydrophila. Development of these compounds may contribute to the development of herbal medicines in aquaculture.

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Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene

Cited by 11 publications

References 26 publications

Aeromonas veronii virulence and adhesion attenuation mediated by the gene aodp

Aeromonas veronii virulence and adhesion attenuation mediated by the gene aodp

A First Report of Aeromonas veronii Infection of the Sea Bass, Lateolabrax maculatus in China

Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

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