Nutritional Requirements of Salmonella Paratyphi and Its Terminal Respiratory Pattern

Abstract

As for the nutritional requirements of Salmonella paratyphi A, Lederberg (1) has already given a short description in his paper on the nutrition of Salmonella. Result observed on him shows that among the total 8 strains of Salmonella parat,yphi A examined, 5 were capable of growing on a simple synthetic medium containing glucose, salts and asparagine, and 2 required tryptophan (or indol) or both methionine and triptophane for growth. The nutritional requirement of the remaining one has not been determined. Recently, Suzuki and Sakaguchi (2) reported that 3 strains of Salmonella paratyphi A grew well on a medium containing glucose, tryptophane cystine and salts. Present authors have examined the cultures of Salmonella paratyphi A, some of which had been kept in their laboratory and the others* Isolated newly in the Komagome Hospital for Infectious Diseases. The experimental results hereof are as summarized in the following. EXPERIMENTAL RESULTS (1) Growth factors required: Among the total 16 atrains of Salmonella paratyphi A examined, 15 were proved to be able to grow in the following simple synthetic culture medium (Medium A) : Glucose 0.2 gm Tryptophane 0.02 gm NaCl 0.5 gm MgSO4 0.02 gm (NH4)H2PO4 0.1 gm K2HPO4 0.1 gm Aq. das. 100 ml * These strains were forwarded to us by courtesy of Dr. K. Kobari of the Komagome Hospital for Infectious Diseases.

Purine Requiring Strains of Shigellae

“…Although several reports (Bhaskaran an(d Rowley, 1956;Fukumi, Sayama, and Nakaya, 1953;Suzuki and Sakaguchi, 1955a, b;Stokes and Bayne, 1958) are available on the purine and pyrimidine requirements for gram-negative bacteria, so far only one strain of Shigella flexneri has been reported to require uracil (Hutner, 1944). Therefore, the finding of two strains of S. boydii, which were purine requiring, but which eould not utilize uracil is worthy of note.…”