This study was conducted to evaluate the physico-chemical properties of three sesame varieties: Adi, Bawnji and T-85. Sesame varieties showed significant (p ≤ 0.05) differences on some physical properties, proximate, mineral, anti-nutritional (phytic acid) and antioxidant compositions. The average values of 1000 seed weight were ranged from (2.74 -3.16 g) and true density from (1190.66 to 1215.58 kg m −3 ). The moisture (wb), crude protein, ash, fat, fiber, total carbohydrate, Ca, Zn and Fe (db) were ranged: 3.17% -3.96%, 22.58% -24.27%, 4.46% -6.19%, 50.88% -52.67%, 5.60% -6.26%, 8.3% -11.69%, 1172.08 -1225.71 mg/100g, 4.23 -4.45 mg/100g and 10.2 -10.75 mg/100g, respectively. Phytic acid contents were ranged from 307.61 to 324.91 mg/100g, total phenolics from (23.16 -25.69 mg GAE/g) and ferric ion reducing power value from (32.33 -34.53 µmol/g) (db). The results were compared with some other sesame varieties grown worldwide. Results showed that Ethiopian sesame varieties were good source in nutrients and were functional foods for human nutrition and utilization.