O efeito do laser de baixa energia no crescimento bacteriano "in vitro"

Coutinho, Fernando; Giordano, Vincenzo; Santos, Carolina Mariano dos; Carneiro, Abel Ferreira; Amaral, Ney Pecegueiro do; Touma, Maria Cristina; Giordano, Mario

doi:10.1590/s0102-36162007000800004

Cited by 7 publications

(9 citation statements)

References 20 publications

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“…12 Coutinho, on the basis of in vitro bacteriological study, evaluated the effect of two types of low-level laser energy on different bacterial populations usually present in post-traumatic wounds. 13 Under the conditions studied, the application of laser did not increase the number of bacterial colonies. 13 Despite advances in the use of low intensity laser on wound healing, the exact biophysical mechanisms of irradiation of LBE in bacterial cells in vivo have not been thoroughly investigated, thus the use of the method remains controversial in cases of suspected or confirmed contamination of soft tissue wounds.…”

Section: Introductionmentioning

confidence: 82%

Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats*

Silva

Plapler

Costa

et al. 2013

An. Bras. Dermatol.

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BACKGROUNDLaser therapy is a low cost, non-invasive procedure with good healing results. Doubts exist as to whether laser therapy action on microorganisms can justify research aimed at investigating its possible effects on bacteria-infected wounds. OBJECTIVETo assess the effect of low intensity laser on the rate of bacterial contamination in infected wounds in the skin of rats. METHODSAn experimental study using 56 male Wistar rats. The animals were randomly divided into eight groups of seven each. Those in the "infected" groups were infected by Staphylococcus aureus MRSA in the dorsal region. Red laser diode (AlGaInP) 658nm, 5J/cm2 was used to treat the animals in the "treated" groups in scan for 3 consecutive days. Samples were drawn before inoculating bacteria and following laser treatment. For statistical analysis we used the nonparametric Wilcoxon (paired data) method with a significance level of p <0.05. RESULTSThe statistical analysis of median values showed that the groups submitted to laser treatment had low bacterial proliferation. CONCLUSIONThe laser (AlGaInP), with a dose of 5J/cm2 in both intact skin and in wounds of rats infected with Staphylococcus aureus MRSA, is shown to reduce bacterial proliferation.

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Section: Introductionmentioning

confidence: 82%

Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats*

Silva

Plapler

Costa

et al. 2013

An. Bras. Dermatol.

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“…(9,13) Other research on the laser radiation effect on bacteria and fungi point to biostimulants or proliferative results, postulating that these effects are linked to changes generated by increased energy intake provided by the radiation in the respiratory chain of bactéria. (10,14) However, the exact biophysical mechanisms of radiation of low intensity laser on bacterial and fungal cells have been poorly investigated, making controversial the use in suspected contamination or confirmed cases of injury to soft tissue. (15,16) Moreover, Nussbaum et al (15) and Tiphlova, Karu (17) proposed that the photobiological response of an organism to exposure to monochromatic light depends directly on irradiation parameters (wavelength, intensity and dose) and culture conditions germ.…”

Section: Discussionmentioning

confidence: 99%

“…The only study in the literature that also analyzed different exposure times was performed by Coutinho et al (14) where lasers with 15W/904nm for 200 seconds, and 20W/904nm for 230 seconds were used in Escherichia coli, Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella sp, Serratia sp and Staphylococcus aureus and it was concluded that in the studied conditions the laser proved to be innocuous regarding the increase in the number of units forming bacterial colonies, at the doses used in this study, indicating that it can be used as an adjuvant in the process wound healing, even in the presence of contamination by bacteria evaluated, confirming the results of this study.…”

Section: Discussionmentioning

confidence: 99%

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Andraus¹,

Maia²,

Santos³

et al. 2015

mtprehabjournal

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Background: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Aim: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Photon Lase III -DMC was used, with the wavelengths of 660 and 808nm and 30mW, 60mW and 100mW of power, with different exposure time to laser irradiation. Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 nterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A 1 , A 2 /B, B 1 , B 2 /C, C 1 , C 2 ), and points A/B/C reciebed only one application, points A 1 ,B 1 ,C 1 received 2 applications and points A 2 ,B 2 ,C 2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. In all the groups, no zone of inhibition nor growth were observed. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

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“…The standardization of equipment and choice of the laser parameters employed, such as wavelength, period, frequency, intensity for tissue repair with concomitant bacterial infection, show great variation in the literature 10,12,15,[19][20][21][22][23][24] . The gallium arsenide laser was chosen to be one of most used lasers in physical therapy practice, capable of 25 .…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

“…Thus, on infected wounds one must consider the cumulative effects of treatment 26 . The choice of in vitro research method was based on previous studies 13,19,24 that performed several experiments evaluating the effects of laser on different types of bacteria, using variations in wavelength, dose and frequency 13,19,21,22,24 . Most studies practiced irradiation of the plates in a single session (common methodological practice in this type of study) 21,24 and bacterial colonies counts were made up to 24 hours afterwards 19,23,24 .…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

Pereira

Paula

Cielinski

et al. 2014

Rev. Col. Bras. Cir.

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Results ResultsResults Results: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). Conclusion Conclusion Conclusion Conclusion Conclusion: lowintensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm 2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment.

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O efeito do laser de baixa energia no crescimento bacteriano "in vitro"

Abstract: RESUMO Objetivo

Cited by 7 publications

References 20 publications

Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats*

Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats*

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

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