O-GlcNAcylation of Mef2c regulates myoblast differentiation

Kim, Han Byeol; Seo, Hyeon Gyu; Son, SeongJin; Choi, Hong-Seok; Kim, Byung Gyu; Kweon, Tae Hyun; Kim, Sung Hoon; Pai, Jaeyoung; Shin, Injae; Yang, Won Ho; Cho, Jin Won

doi:10.1016/j.bbrc.2020.06.031

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Protein O -linked β- N -acetylglucosamine ( O -GlcNAcylation) negatively regulates the C2C12 cell myogenic program [ 6 ]. Moreover, Mef2c, the transcription factor of myogenin, is O -GlcNAcylated, and O -GlcNAcylation of Thr9 in Mef2c inhibits its DNA binding affinity [ 7 ]. However, the association between cellular differentiation and other glycosylations is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

et al. 2021

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DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

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Section: Introductionmentioning

confidence: 99%

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

et al. 2021

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“…Loumaye A et al reported that MEF2C was able to maintain the slow expression and protein content of the myosin heavy chain beta(MyHC- ) subtype in differentiated myotubes [ 41 ]. Moreover, Kim HB et al (2020) reported that O-GlcNAcylation of MEF2C was necessary for regulation of myoblast differentiation [ 42 ]. Interestingly, MEF2C was also enriched in the MAPK signalling pathway.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of the DNA methylome and RNA transcriptome during the development of skeletal muscle in Duroc pigs

Li,

Wang,

Chen

et al. 2024

BMC Genomics

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Background Skeletal muscle development plays a crucial role in yield and quality of pork; however, this process is influenced by various factors. In this study, we employed whole-genome bisulfite sequencing (WGBS) and transcriptome sequencing to comprehensively investigate the longissimus dorsi muscle (LDM), aiming to identify key genes that impact the growth and development of Duroc pigs with different average daily gains (ADGs). Results Eight pigs were selected and divided into two groups based on ADGs: H (774.89 g) group and L (658.77 g) group. Each pair of the H and L groups were half-siblings. The results of methylation sequencing revealed 2631 differentially methylated genes (DMGs) involved in metabolic processes, signalling, insulin secretion, and other biological activities. Furthermore, a joint analysis was conducted on these DMGs and the differentially expressed genes (DEGs) obtained from transcriptome sequencing of the same individual. This analysis identified 316 differentially methylated and differentially expressed genes (DMEGs), including 18 DMEGs in promoter regions and 294 DMEGs in gene body regions. Finally, LPAR1 and MEF2C were selected as candidate genes associated with muscle development. Bisulfite sequencing PCR (BSP) and quantitative real-time PCR (qRT–PCR) revealed that the promoter region of LPAR1 exhibited significantly lower methylation levels (P < 0.05) and greater expression levels (P < 0.05) in the H group than in the L group. Additionally, hypermethylation was observed in the gene body region of MEF2C, as was a low expression level, in the H group (P < 0.05). Conclusions These results suggest that the differences in the ADGs of Duroc pigs fed the same diet may be influenced by the methylation levels and expression levels of genes related to skeletal muscle development.

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“…For example, the global O -GlcNAcylation reduced in early myogenesis before myoblast fusion and the inactivation of OGA severely interferes with the expression of myogenin and myogenic regulatory factor 4, which indicate that the terminal differentiation program of skeletal myogenesis is negatively regulated by O -GlcNAcylation [ 193 ]. Hyper O -GlcNAcylation of myocyte-specific enhancer factor 2c at Thr 9 inhibits its DNA binding affinity to the myogenin promoter to attenuate skeletal muscle differentiation [ 194 ]. This negative regulation was also eliminated by hypo O -GlcNAcylation of myocyte-specific enhancer factor 2d [ 195 ].…”

Section: The Fine Characteristics Of O -Glcnacylat...mentioning

confidence: 99%

Protein O-GlcNAcylation in Metabolic Modulation of Skeletal Muscle: A Bright but Long Way to Go

Liu

2022

Metabolites

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O-GlcNAcylation is an atypical, dynamic and reversible O-glycosylation that is critical and abundant in metazoan. O-GlcNAcylation coordinates and receives various signaling inputs such as nutrients and stresses, thus spatiotemporally regulating the activity, stability, localization and interaction of target proteins to participate in cellular physiological functions. Our review discusses in depth the involvement of O-GlcNAcylation in the precise regulation of skeletal muscle metabolism, such as glucose homeostasis, insulin sensitivity, tricarboxylic acid cycle and mitochondrial biogenesis. The complex interaction and precise modulation of O-GlcNAcylation in these nutritional pathways of skeletal muscle also provide emerging mechanical information on how nutrients affect health, exercise and disease. Meanwhile, we explored the potential role of O-GlcNAcylation in skeletal muscle pathology and focused on its benefits in maintaining proteostasis under atrophy. In general, these understandings of O-GlcNAcylation are conducive to providing new insights into skeletal muscle (patho) physiology.

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O-GlcNAcylation of Mef2c regulates myoblast differentiation

Cited by 7 publications

References 20 publications

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

Integrated analysis of the DNA methylome and RNA transcriptome during the development of skeletal muscle in Duroc pigs

Protein O-GlcNAcylation in Metabolic Modulation of Skeletal Muscle: A Bright but Long Way to Go

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