O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

Peng, Zhong; Liang, Wan; Hu, Zizhe; Li, Xiaosong; Guo, Rui; Lin, Hua; Tang, Xibiao; Tan, Chen; Chen, Huanchun; Wang, Xiangru; Wu, Bin

doi:10.1186/s12917-019-2177-1

Cited by 29 publications

(22 citation statements)

References 70 publications

(75 reference statements)

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“…It reveals that there is diversity in ST types in Hubei province. In some other surveys, ST101 (7/53, 13.21%) [22] and ST101 (3/32, 9.4%) [14] were common STs, these are similar to our result ST101 (3/29, 10.3%). In Yang′s study, the common ST was ST10 (22/171, 12.9%), followed by ST744 (8/171, 4.7%), ST101 (7/171, 4.1%), ST165 (5/171, 2.9%) [17] .…”

Section: Discussionsupporting

confidence: 93%

Genotypic Diversity and Antimicrobial Resistance of Escherichia Coli Isolated From Pigs in Hubei, China

Liu

Wang

et al. 2021

Preprint

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BackgroundIntestinal infections with Escherichia coli (E. coli) are mostly occur in piglets between 1~10 days old, which major lead to diarrhea and edema in newborn piglets. These diseases caused by E. coli can increase mortality, morbidity and growth delay of piglets, which are responsible for economic losses. In this study was to investigate the prevalence of antibiotic resistance, transmission mechanisms, and molecular epidemiology of E. coli strains isolated from pig farms in Hubei province. Furthermore, clonal and genetic diversity of isolates were identified. ResultsA total of 29 E. coli isolates were obtained from fecal of weaned piglets from Hubei province. The E. coli isolates in different regions demonstrate different genetic diversity. Multilocus sequence typing (MLST) presented that ST165 was the common sequence type, accounting for 27.6% of all E. coli isolates, followed by ST744, ST1081, ST101 and ST10. All of the isolates were resistant to the tested antibiotics to vary degrees, and more than 80% of E. coli isolates presented high resistance rates to ampicillin, lincomycin, doxycycline, tetracycline, sulfaisoxazole and ampicillin. There was one E. coli strain that was resistant to the fifteen antimicrobial agents tested. Overall, most of the isolates were conferring resistance to 5-7 antimicrobial agents tested.ConclusionsOur study reported E. coli isolates with high antimicrobial resistance and explores the genetic diversity of E. coli isolated from swine-origin. From the results obtained it can be concluded that these isolates present high prevalent multi-drug resistance. These data provide a greater understanding of the genetic diversity and antimicrobial resistance of E. coli.

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Section: Discussionsupporting

confidence: 93%

Genotypic Diversity and Antimicrobial Resistance of Escherichia Coli Isolated From Pigs in Hubei, China

Liu

Wang

et al. 2021

Preprint

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“…Among seven strains, no typical pathogenicity markers were detected, indicating that these strains do not belong to the most common pathotypes. An exception is represented by the enterohemolysin coding gene ehxA, frequently detected on plasmids in EHEC strains and frequently reported to be associated with stx genes (Beutin et al, 1989;Peng et al, 2019) and with the possibility to develop severe diseases (Franz et al, 2015). Moreover, the investigation of other virulence determinants showed the presence of adhesins and genes belonging to the serine protease autotransporters of Enterobacteriaceae (SPATE) family in all strains.…”

Section: Discussionmentioning

confidence: 99%

Genomic Investigation of Virulence Potential in Shiga Toxin Escherichia coli (STEC) Strains From a Semi-Hard Raw Milk Cheese

et al. 2021

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Shiga-toxin-producing Escherichia coli (STEC) represents a significant cause of foodborne disease. In the last years, an increasing number of STEC infections associated with the consumption of raw and pasteurized milk cheese have been reported, contributing to raise the public awareness. The aim of this study is to evaluate the main genomic features of STEC strains isolated from a semi-hard raw milk cheese, focusing on their pathogenic potential. The analysis of 75 cheese samples collected during the period between April 2019 and January 2020 led to the isolation of seven strains from four stx-positive enrichment. The genome investigation evidenced the persistence of two serotypes, O174:H2 and O116:H48. All strains carried at least one stx gene and were negative for eae gene. The virulence gene pattern was homogeneous among the serogroup/ST and included adherence factors (lpfA, iha, ompT, papC, saa, sab, hra, and hes), enterohemolysin (ehxA), serum resistance (iss, tra), cytotoxin-encoding genes like epeA and espP, and the Locus of Adhesion and Autoaggregation Pathogenicity Islands (LAA PAIs) typically found in Locus of Enterocyte Effacement (LEE)-negative STEC. Genome plasticity indicators, namely, prophagic sequences carrying stx genes and plasmid replicons, were detected, leading to the possibility to share virulence determinants with other strains. Overall, our work adds new knowledge on STEC monitoring in raw milk dairy products, underlining the fundamental role of whole genome sequencing (WGS) for typing these unknown isolates. Since, up to now, some details about STEC pathogenesis mechanism is lacking, the continuous monitoring in order to protect human health and increase knowledge about STEC genetic features becomes essential.

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“…Some of the serogroups detected in cattle feces such as O5, O8, O9, O11, O15, O20, O49, O59, O62, O65, O69, O71, O76, O78, O86, O87, O89, O91, O100, O114, O115, O116, O119, O120, O128, O138, O139, O141, O143, O147, O159, O163, O167, O172, O174, and O180 have also been detected in swine feces (Cha et al, 2018 ; Peng et al, 2019 ). A few of the swine STEC serogroups, particularly O8, O138, O139, O141, and O147, are more often implicated in edema disease in weaned piglets and young finishing pigs (Kaper et al, 2004 ; Melton-Celsa et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Multiplex PCR Assays for the Detection of One Hundred and Thirty Seven Serogroups of Shiga Toxin-Producing Escherichia coli Associated With Cattle

Ludwig

Shi

Shridhar

et al. 2020

Front. Cell. Infect. Microbiol.

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Escherichia coli carrying prophage with genes that encode for Shiga toxins are categorized as Shiga toxin-producing E. coli (STEC) pathotype. Illnesses caused by STEC in humans, which are often foodborne, range from mild to bloody diarrhea with life-threatening complications of renal failure and hemolytic uremic syndrome and even death, particularly in children. As many as 158 of the total 187 serogroups of E. coli are known to carry Shiga toxin genes, which makes STEC a major pathotype of E. coli. Seven STEC serogroups, called top-7, which include O26, O45, O103, O111, O121, O145, and O157, are responsible for the majority of the STEC-associated human illnesses. The STEC serogroups, other than the top-7, called "non-top-7" have also been associated with human illnesses, more often as sporadic infections. Ruminants, particularly cattle, are principal reservoirs of STEC and harbor the organisms in the hindgut and shed in the feces, which serves as a major source of food and water contaminations. A number of studies have reported on the fecal prevalence of top-7 STEC in cattle feces. However, there is paucity of data on the prevalence of non-top-7 STEC serogroups in cattle feces, generally because of lack of validated detection methods. The objective of our study was to develop and validate 14 sets of multiplex PCR (mPCR) assays targeting serogroup-specific genes to detect 137 non-top-7 STEC serogroups previously reported to be present in cattle feces. Each assay included 7-12 serogroups and primers were designed to amplify the target genes with distinct amplicon sizes for each serogroup that can be readily identified within each assay. The assays were validated with 460 strains of known serogroups. The multiplex PCR assays designed in our study can be readily adapted by most laboratories for rapid identification of strains belonging to the non-top-7 STEC serogroups associated with cattle.

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O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

Cited by 29 publications

References 70 publications

Genotypic Diversity and Antimicrobial Resistance of Escherichia Coli Isolated From Pigs in Hubei, China

Genotypic Diversity and Antimicrobial Resistance of Escherichia Coli Isolated From Pigs in Hubei, China

Genomic Investigation of Virulence Potential in Shiga Toxin Escherichia coli (STEC) Strains From a Semi-Hard Raw Milk Cheese

Multiplex PCR Assays for the Detection of One Hundred and Thirty Seven Serogroups of Shiga Toxin-Producing Escherichia coli Associated With Cattle

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