Objectivity in the classification of tumours of the nasal epithelium

Abstract

Summary A survey of tumours derived from each of the four cell types of nasal epithelium is presented. Criticism is levelled at the adoption of additional terms for tissue types such as lympho-epithelium and transitional cell epithelium and tumours said to be derived from them. Electron microscopy is of assistance in classification particularly in the detection of evidence of keratin synthesis. The proposed classification of tumours of the nasal epithelium is: (1) Pseudostratified columnar epithelium: (a) papillary adenoma, (b) papillary carcinoma. (2) Squamous epithelium: (a) everted squamous papilloma, (b) inverted papilloma, (c) squamous carcinoma of any grade of differentiation from well differentiated to undifferentiated. (3) Melanocyte: malignant melanoma. (4) Olfactory neuroepithelium: olfactory neuroblastoma.

Sperm protein 17 expression defines 2 subsets of primary esthesioneuroblastoma

“…This difference ( P = .035) extends previous in vitro findings showing a significant increase in Sp17 mRNA in 3 metastatic cell lines derived from the keratinocyte cell line Pam 212 after in vivo tumor progression [23]. The comparative analysis between Sp17 expression and the well-known markers of this rare neoplasia [1,19,20] has shown a statistically significant relation between Sp17 and chromogranin expression ( P = .014), whereas a nonsignificant relation was found between Sp17 expression and S-100, synaptophysin, and neurofilament markers (Table 3). A nonsignificant relation was found between Sp17 expression and the proliferative capacity of the lesion, as demonstrated by the Ki-67 expression (Table 3).…”

“…The lesions were histologically graded using the system of Hyams and Michaels [20]: 12 patients (29%) had grade 1 disease; 14 (33%) grade 2; 10 (24%) grade 3; and 5 (12%) grade 4. One patient (2%) was not graded.…”

“…All of the patients were staged according to the Kadish and Morita staging systems [18,19], and the lesions were histologically graded based on the diagnostic criteria proposed by Hyams and Michaels [20].…”

“…After deparaffinization and rehydration, the sections were immersed in a bath (Dako, Milan, Italy) for antigen retrieval (Table 1), incubated with 3% H 2 O 2 for 15 minutes to quench endogenous peroxidase activity, and then treated for 2 hours at room temperature with primary antibodies raised against human Sp17 (monoclonal mouse anti-Sp17) [7], Ki-67 (Dako), chromogranin (Dako) [19], synaptophysin (DBA, Milan, Italy) [1], S-100 (Dako) [20], neurofilament (Novocastra, Milan, Italy) [20], or with 1 mg/mL mouse IgG1 (Dako) as a negative control. This was followed by 30 minutes of incubation with the Dako Envision system, which is based on a unique enzyme-conjugated polymer backbone that also carries secondary antibody molecules (Dako).…”