Observation of arenavirus nucleoprotein heptamer assembly

Papageorgiou, Nicolas; Vaitsopoulou, Afroditi; Diop, Awa; Nguyen, Thi Hong Van; Canard, Bruno; Alvarez, Karine; Ferrón, François

doi:10.1002/2211-5463.13106

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Unlike previously documented arenaviruses, Trematomus arenavirus , had its nucleoprotein sequence split over two non-overlapping ORFs. Arenavirus nucleoproteins (NPs) are well-conserved at both sequence and structural levels and are multifunctional, comprising a core domain that polymerises and protects the viral RNA and an exonuclease domain for degrading dsRNA, connected by “flexible” linkers (Papageorgiou et al, 2021). The split in the NP of Trematomus arenavirus occurred in the poorly conserved linker region between the two domains, which also contained an α-helix structure modelled with high confidence, uncommon in known arenaviruses and present in three of the four newly discovered fish arenaviruses.…”

Section: Discussionmentioning

confidence: 99%

Viromes of Antarctic fish resembles the diversity found at lower latitudes

Grimwood,

Waller,

Wierenga

et al. 2024

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Antarctica harbours some of the most isolated and extreme environments on Earth, concealing a largely unexplored and unique component of the global animal virosphere. To understand the diversity and evolutionary histories of viruses in these polar species we determined the viromes of 11 Antarctic fish species with 248 samples collected from the Ross Sea region spanning the Perciformes, Gadiformes, and Scorpaeniformes orders. The continent's shift southward and cooling temperatures over 20 million years ago led to a reduction in biodiversity and subsequent radiation of some marine fauna, such as the notothenioid fishes. Despite decreased host species richness in polar regions, we revealed a surprisingly complex virome diversity in Ross Sea fish, with the types and numbers of viruses per host species and individuals sampled comparable to that of fish in warmer marine environments with higher host community diversity. We also observed a higher number of closely related viruses likely representing instances of recent and historic host-switching events among Perciformes (all notothenioids) than in the Gadiformes, suggesting that rapid speciation events within this order generated closely related host species with few genetic barriers to cross-species transmission. Additionally, we identified novel genomic variation in an arenavirus with a split nucleoprotein sequence containing a stable helical structure, indicating potential adaptation of viral proteins to extreme temperatures. These findings enhance our understanding of virus evolution and virus-host interactions in response to environmental shifts, especially in less diverse ecosystems more vulnerable to the impacts of anthropogenic and climate changes.

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Section: Discussionmentioning

confidence: 99%

Viromes of Antarctic fish resembles the diversity found at lower latitudes

Grimwood,

Waller,

Wierenga

et al. 2024

Preprint

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“…Any alteration of the remaining ion impairs greatly theses activities [2]. We present a global study aiming to characterize the assembly of the NP [3], through flexible domains [4], a step critical for vRNApackaging and the polsitioning of L for vRNA replication, as well as using a combined approach of biophysical screening, crystallography and in silico docking, identifying active compounds against both nucleases [5]. Crystal structures of the nucleases domain complexed with several compounds were obtained [67].…”

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confidence: 99%