Observation of Hydrofluoric Acid Burns on Osseous Tissues by Means of Terahertz Spectroscopic Imaging

Baughman, William; Yokus, H.; Balci, Soner; Wilbert, David S.; Kung, Patrick; Kim, S. M.

doi:10.1109/jbhi.2013.2243158

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…Investigations using a THz transmission setup have also shown the potential of applying spectroscopy across an imaging scan in order to perform regional solutions of the properties of the sample under test. This concept has been applied for assessment of crystal formations in pharmaceuticals [22] and in biomedical imaging for observing the degradation of bone tissue [23].…”

Section: Introductionmentioning

confidence: 99%

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Bowman

El-Shenawee

Campbell

2016

Biomed. Opt. Express

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This work presents experimental and analytical comparison of terahertz transmission and reflection imaging modes for assessing breast carcinoma in excised paraffinembedded human breast tissue. Modeling for both transmission and reflection imaging is developed. The refractive index and absorption coefficient of the tissue samples are obtained. The reflection measurements taken at the system's fixed oblique angle of 30° are shown to be a hybridization of TE and TM modes. The models are validated with transmission spectroscopy at fixed points on fresh bovine muscle and fat tissues. Images based on the calculated absorption coefficient and index of refraction of bovine tissue are successfully compared with the terahertz magnitude and phase measured in the reflection mode. The validated techniques are extended to 20 and 30 μm slices of fixed human lobular carcinoma and infiltrating ductal carcinoma mounted on polystyrene microscope slides in order to investigate the terahertz differentiation of the carcinoma with non-cancerous tissue. Both transmission and reflection imaging show clear differentiation in carcinoma versus healthy tissue. However, when using the reflection mode, in the calculation of the thin tissue properties, the absorption is shown to be sensitive to small phase variations that arise due to deviations in slide and tissue thickness and non-ideal tissue adhesion. On the other hand, the results show that the transmission mode is much less sensitive to these phase variations. The results also demonstrate that reflection imaging provides higher resolution and more clear margins between cancerous and fibroglandular regions, cancerous and fatty regions, and fibroglandular and fatty tissue regions. In addition, more features consistent with high power pathology images are exhibited in the reflection mode images.

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Section: Introductionmentioning

confidence: 99%

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Bowman

El-Shenawee

Campbell

2016

Biomed. Opt. Express

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“…Terahertz (THz) imaging technology has been a growing area of interest for biomedical applications. 1 For example, pulsed THz systems have been used to conduct imaging of liver cirrhosis, 2 osseous tissue damage, 3 and differentiation of cancerous tissues of the brain, 4 stomach, 5 colon, 6 and breast, 7-10 among others. However, imaging of breast and other cancers has been mostly limited to formalin-fixed, paraffin-embedded (FFPE) tissue to date, with only a few studies working with freshly excised tissue due to the challenges of obtaining fresh tissue outside of a surgical setting.…”

Section: Introductionmentioning

confidence: 99%

Pulsed terahertz imaging of breast cancer in freshly excised murine tumors

et al. 2018

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This paper investigates terahertz (THz) imaging and classification of freshly excised murine xenograft breast cancer tumors. These tumors are grown via injection of E0771 breast adenocarcinoma cells into the flank of mice maintained on high-fat diet. Within 1 h of excision, the tumor and adjacent tissues are imaged using a pulsed THz system in the reflection mode. The THz images are classified using a statistical Bayesian mixture model with unsupervised and supervised approaches. Correlation with digitized pathology images is conducted using classification images assigned by a modal class decision rule. The corresponding receiver operating characteristic curves are obtained based on the classification results. A total of 13 tumor samples obtained from 9 tumors are investigated. The results show good correlation of THz images with pathology results in all samples of cancer and fat tissues. For tumor samples of cancer, fat, and muscle tissues, THz images show reasonable correlation with pathology where the primary challenge lies in the overlapping dielectric properties of cancer and muscle tissues. The use of a supervised regression approach shows improvement in the classification images although not consistently in all tissue regions. Advancing THz imaging of breast tumors from mice and the development of accurate statistical models will ultimately progress the technique for the assessment of human breast tumor margins.

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“…Terahertz (THz) electromagnetic spectrum ranges from tens of gigahertz (GHz) to several THz. The vibrational and rotational energy levels of large molecules, especially biological macromolecules mostly lie in the THz wave band [15,16]. Biological molecular vibrations and rotations involve intra/inter molecular weak hydrogen bonds, van der Waals forces, conformational changes, non-bonded hydrophobic and hydrophilic interactions [17,18].…”

Section: Introductionmentioning

confidence: 99%

Label-free protein detection using terahertz time-domain spectroscopy

Han

Yan

Zang

et al. 2018

Biomed. Opt. Express

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Protein analysis is the foundation to understanding the mechanisms of complex biological processes. As one of the most widely used techniques to determine protein species and contents, protein dot blot aids biology research but needs corresponding antibodies for marking. A label-free detection method based on terahertz time-domain spectroscopy (THz-TDS) is proposed and demonstrated to improve this traditional technology. A membrane loaded with protein samples is directly scanned using a transmission THz-TDS system for spectral imaging. Different kinds of proteins can be distinguished by the refractive index extracted from the THz transmission spectrum. The intensity or shade imaged with the THz transmission spectrum can help detect the protein quantitatively. The feasibility of this new protein assay is demonstrated by the results of systematic testing with actual samples prepared with the dot-blot protocol.

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Observation of Hydrofluoric Acid Burns on Osseous Tissues by Means of Terahertz Spectroscopic Imaging

Cited by 12 publications

References 23 publications

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Pulsed terahertz imaging of breast cancer in freshly excised murine tumors

Label-free protein detection using terahertz time-domain spectroscopy

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