Observation of nanoparticle internalization on cellular membranes by using noninterferometric widefield optical profilometry

Wang, Chun‐Chieh; Lee, Chia‐Wei; Huang, Chia-Yun; Lin, Jiunn-Yuan; Wei, Pei‐Kuen; Lee, Chau‐Hwang

doi:10.1364/ao.47.002458

Cited by 7 publications

(2 citation statements)

References 21 publications

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“…From this study, we found that direct luminescence‐based imaging of GNPs can hold a valuable and promising position in understanding their accumulation kinetics and distribution of GNPs at the intra‐organ level. We anticipate that the techniques demonstrated in the current study could extend the longitudinal in vitro studies 43, 44 to an in vivo setting whereby one could understand the targeting kinetics (i.e., cell internalization, particle transport, and cell‐specific targeting) in a more realistic solid tumor environment. In turn, this information will be valuable to develop models for generating thermal dosimetry of GNPs in therapeutic settings.…”

Section: Discussionmentioning

confidence: 98%

Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

et al. 2010

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Background and Objectives-Gold nanoparticles (GNPs) such as gold nanoshells (GNSs) and gold nanorods (GNRs) have been explored in a number of in vitro and in vivo studies as imaging contrast and cancer therapy agents due to their highly desirable spectral and molecular properties. While the organ-level biodistribution of these particles has been reported previously, little is known about the cellular level or intra-organ biodistribution. The objective of this study was to demonstrate the use of intrinsic two-photon induced photoluminescence (TPIP) to study the cellular level biodistribution of GNPs.

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Section: Discussionmentioning

confidence: 98%

Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

et al. 2010

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“…The depth positioning accuracy of the liposomes is thus about 17 nm. More details about the calibration of axial positions of scattering particles by the NIWOP technique can be found in a previous publication (Wang et al, 2008).…”

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confidence: 99%

Three‐dimensional tracking and temporal analysis of liposomal transport in live cells using bright‐field imaging

Tsai

Tai

Wang

et al. 2010

Microscopy Res & Technique

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Gold nanoparticles (AuNPs) confined in liposomes of diameters around 200 nm produce strong scattering signal owing to surface plasmon resonance, and therefore bright-field optical tracking of the AuNP-encapsulating liposomes can be conducted in living cells. Using an optical profiling technique called noninterferometric wide-field optical profilometry and a bright-field tracking algorithm, the polynomial-fit Gaussian weight method, we analyze three-dimensional (3D) motion of such liposomes in living fibroblasts. The positioning accuracy in three dimensions is nearly 20 nm. We tag the liposome membranes with fibroblast growth factor-1 and reveal the intracellular transportation processes toward or away from the nucleus. On the basis of a temporal analysis of the intracellular 3D trajectories of AuNP-encapsulating liposomes, we identify directed and diffusive motions in the transportation processes.

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Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

et al. 2009

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The sulfated glycosaminoglycans (sGAG) heparin and chondroitin sulfate (CS) were immobilized on the surfaces of gold nanorods as part of a polyelectrolyte multilayer. The effects of these nanomaterials on the self-assembly of type I collagen were examined by turbidity assays and microscopy, and desorption of sGAG from nanomaterial-collagen composites was quantified biochemically. The interactions of sGAG-coated nanorods with cardiac cells were also explored through a collagen gel contraction assay and confocal microscopy. In contrast to soluble forms of sGAG, sGAG-coated nanorods consistently accelerated collagen fibrillogenesis. Soluble heparin, and heparin-and CS-coated nanorods inhibited cell-mediated contraction of collagen gels, whereas soluble CS did not. Both heparin and CS-coated nanorods were detected in the peri-and/or intra-cellular compartments of the cells, but there was no evidence of cytotoxicity over 72 h of culture. These results indicate that biological polyanions, such as sGAG, may be useful in the modification of nanoparticle surface chemistry for biological and/or therapeutic applications.

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Observation of nanoparticle internalization on cellular membranes by using noninterferometric widefield optical profilometry

Cited by 7 publications

References 21 publications

Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

Three‐dimensional tracking and temporal analysis of liposomal transport in live cells using bright‐field imaging

Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

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