We present experimental results on a Bose gas in a quasi-2D geometry near the Berezinskii, Kosterlitz and Thouless (BKT) transition temperature. By measuring the density profile, in situ and after time of flight, and the coherence length, we identify different states of the gas. In particular, we observe that the gas develops a bimodal distribution without long range order. In this state, the gas presents a longer coherence length than the thermal cloud; it is quasi-condensed but is not superfluid. Experimental evidence indicates that we observe the superfluid transition (BKT transition).PACS numbers: 03.75. Lm, 64.70.Tg One of the most fascinating aspects of a Bose gas in the degenerate regime is the role of dimensionality. A 2D interacting Bose gas is superfluid at low enough temperature [1,2]. However, by contrast to the 3D case, there is no long range coherence and the coherence decays as a power law [1,2]. At temperatures above the Berezinskii-Kosterlitz-Thouless (BKT) transition temperature, the gas is not superfluid. Due to proliferation of free vortices, the quasi-condensate (QC) is fractured into small regions of nearly uniform phase, whose size, which corresponds to the typical length of the exponential decay of the coherence, is larger than the thermal de Broglie wavelength (λ = 2π 2 /M k B T , where T is the temperature and M the atomic mass). For higher T, this size becomes smaller and approaches λ, as the gas crosses over to the thermal phase.Experiments on 2D bosonic systems, such as twodimensional 4 He films [3] and trapped Bose gases [4,5], are able to show signatures of the BKT transition. Other systems, such as the superconducting transition in arrays of Josephson junctions [6] and a two dimensional lattice of (3D) Bose-Einstein condensates [7], also exhibit a similar transition. Another interesting observation was in two dimensional spin polarized atomic hydrogen on liquid 4 He [8] where a reduction in three-body dipolar recombination (which is usually associated with condensation) was observed well above the BKT transition temperature. This observation results from a reduction of density fluctuations, which corresponds to quasi-condensation [9] [10].In this letter, we present evidence of transitions in a quasi-2D Bose gas from thermal (normal gas), to quasicondensate without superfluidity, to superfluid quasicondensate (BKT transition). We explicitly identify the theoretically expected non-superfluid quasi-condensate, a feature not clearly seen in other experiments on a 2D trapped Bose gases [4,5]. We use an interferometric method to study the coherence of the gas down to distances smaller than the thermal de Broglie wavelength. Our results can be understood using the local density approximation (LDA) on a model [11] of a homogeneous system. More recently, calculations for a trapped system have been carried out using classical-quantum field methods [12] and quantum Monte Carlo methods [13].The BKT transition occurs at a universal value of the superfluid density n s = 4/λ 2 . However, the ...