Upon varying discharge current and gap width, several self‐organized patterns are observed in the anode layer of an atmospheric pressure glow discharge with miniature axial argon flow, including diffuse‐disk, single‐ring, disk‐ring, wheel‐spokes, and radial‐stripes patterns. Among them, the wheel‐spokes pattern is observed for the first time in atmospheric pressure glow discharge above a water anode. The conditions for generating the self‐organized patterns are also investigated. Fast photography results indicate that the patterns with low current and narrow gap are diffuse in nature, while those with high current and wide gap are composed of individual spots. This is further verified from waveforms of applied voltage and light emission signals. Moreover, the generation, movement, and disappearance of discrete spots are observed by a high‐speed video camera. Besides, the optical emission spectrum indicates that the glow discharge above the water surface is abundant with electronegative species, including O, NO, NH, and OH. Finally, the formation mechanism of diffuse and spotted anode layers is revealed.